PURIFICATION AND IDENTIFICATION OF THE ANTIGEN ASSOCIATED WITH MONOCLONAL ANTIBODY 3H<sub>11</sub> AGAINST GASTRIC CANCER CELL

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PURIFICATION AND IDENTIFICATION OF THE ANTIGEN ASSOCIATED WITH MONOCLONAL ANTIBODY 3H₁₁ AGAINST GASTRIC CANCER CELL

中国医学科学杂志（英文版） 1998年0卷第3期页码：150-153

Updated：2024-04-10

- PURIFICATION AND IDENTIFICATION OF THE ANTIGEN ASSOCIATED WITH MONOCLONAL ANTIBODY 3H₁₁ AGAINST GASTRIC CANCER CELL
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  中图分类号： R735.2
- 纸质出版日期：1998
- Accepted：
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PURIFICATION AND IDENTIFICATION OF THE ANTIGEN ASSOCIATED WITH MONOCLONAL ANTIBODY 3H₁₁ AGAINST GASTRIC CANCER CELL[J]. 中国医学科学杂志（英文版）, 1998,0(3):150-153.

PURIFICATION AND IDENTIFICATION OF THE ANTIGEN ASSOCIATED WITH MONOCLONAL ANTIBODY 3H₁₁ AGAINST GASTRIC CANCER CELL[J]. Chinese medical sciences journal, 1998, 0(3): 150-153.
PURIFICATION AND IDENTIFICATION OF THE ANTIGEN ASSOCIATED WITH MONOCLONAL ANTIBODY 3H₁₁ AGAINST GASTRIC CANCER CELL[J]. 中国医学科学杂志（英文版）, 1998,0(3):150-153. DOI：

PURIFICATION AND IDENTIFICATION OF THE ANTIGEN ASSOCIATED WITH MONOCLONAL ANTIBODY 3H₁₁ AGAINST GASTRIC CANCER CELL[J]. Chinese medical sciences journal, 1998, 0(3): 150-153. DOI：

摘要

Abstract

The object of this study is to investigate the properties of gastric cancer assoiciated antigen by McAb3H

against gastric cancer cell line. Antigen purified by affinity chromatography and further charac- terized by biochemical and immunological techiques. Results showed that assoiciated antigen of McAb 3H

was mainly located on the membrane of target cells. It was sensitive to heat and digestion of proteases

but resistant to treatment with sodium periodate. Schiff’s reagent staining was negative. SDS-PAGE and IEF- analysis showed that the antigen was a 210 kD molecular weight with pl of 8. 5 protein. The molecular weight of antigen extracted from target cell with tunicamycin was not changed. We concluded that the cor- responding antigen of McAb 3H

might be an alkaline protein.

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PURIFICATION AND IDENTIFICATION OF THE ANTIGEN ASSOCIATED WITH MONOCLONAL ANTIBODY 3H11 AGAINST GASTRIC CANCER CELL

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PURIFICATION AND IDENTIFICATION OF THE ANTIGEN ASSOCIATED WITH MONOCLONAL ANTIBODY 3H₁₁ AGAINST GASTRIC CANCER CELL