SUPPRESSION OF MALIGNANT PHENOTYPE OF A TRANSFORMED MOUSE MAMMARY EPITHELIAL CELL LINE （11A1）BY TUMOR SUPPRESSOR GENE RB

王冬梅; 李申德; 崔惠云; 杨晓洁

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SUPPRESSION OF MALIGNANT PHENOTYPE OF A TRANSFORMED MOUSE MAMMARY EPITHELIAL CELL LINE （11A1）BY TUMOR SUPPRESSOR GENE RB

中国医学科学杂志（英文版） 1997年第2期页码：76-79

Updated：2024-04-10

- SUPPRESSION OF MALIGNANT PHENOTYPE OF A TRANSFORMED MOUSE MAMMARY EPITHELIAL CELL LINE （11A1）BY TUMOR SUPPRESSOR GENE RB
- Affiliations：
  
  1. Cancer Institute
  2. CAMS & PUMC
  3. ,China
- Author bio：
- Funds：
- DOI：
  中图分类号： R73-3
- 纸质出版日期：1997
- Accepted：
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[1]王冬梅,李申德,崔惠云,杨晓洁.SUPPRESSION OF MALIGNANT PHENOTYPE OF A TRANSFORMED MOUSE MAMMARY EPITHELIAL CELL LINE (11A1)BY TUMOR SUPPRESSOR GENE RB[J].Chinese Medical Sciences Journal,1997(02):76-79.

SUPPRESSION OF MALIGNANT PHENOTYPE OF A TRANSFORMED MOUSE MAMMARY EPITHELIAL CELL LINE （11A1）BY TUMOR SUPPRESSOR GENE RB[J]. Chinese medical sciences journal, 1997, (2): 76-79.
[1]王冬梅,李申德,崔惠云,杨晓洁.SUPPRESSION OF MALIGNANT PHENOTYPE OF A TRANSFORMED MOUSE MAMMARY EPITHELIAL CELL LINE (11A1)BY TUMOR SUPPRESSOR GENE RB[J].Chinese Medical Sciences Journal,1997(02):76-79. DOI：

SUPPRESSION OF MALIGNANT PHENOTYPE OF A TRANSFORMED MOUSE MAMMARY EPITHELIAL CELL LINE （11A1）BY TUMOR SUPPRESSOR GENE RB[J]. Chinese medical sciences journal, 1997, (2): 76-79. DOI：

摘要

<正> A malignant transformed mammary epithelial cell line （11A1） was transfected with liposome encapsulated eukaryotic expression plasmid pCMV-neo-RB

yielding 4 constant clones which have obvious pheno-typic reversion changes

and named 11A1-R1-R4 respectively. Further experiments showed that the 11A1-R1 behaved like normal epithelial cells in both morphological and biological characteristics

with decreased clonogenicity in solid argar medium as well as decreased tumorigenicity. Northern blot hybridization showed increased expression of RB gene and decreased expression of c-myc gene in 11A1-R1

11A1-R2 cells compared to 11A1 cells. This was an ideal phenotypic reversion model for epithelial transformed cell line and demonstrated that the RB gene can reexpress and suppress malignant phenotype in RB inactive cells.

Abstract

A malignant transformed mammary epithelial cell line （11A1） was transfected with liposome encapsulated eukaryotic expression plasmid pCMV-neo-RB

yielding 4 constant clones which have obvious pheno-typic reversion changes

and named 11A1-R1-R4 respectively. Further experiments showed that the 11A1-R1 behaved like normal epithelial cells in both morphological and biological characteristics

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