RESTRICTION　FRAGMENT　LENGTH　POLYMORPHISM（RFLP）　ANALYSIS　OF　GENOMIC　DNA　OF　5　STRAINS　OF　TRICHINELLA　SPIRALIS　IN　CHINA

王虹; 张月清; 劳为德; 吴赵永

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RESTRICTION　FRAGMENT　LENGTH　POLYMORPHISM（RFLP）　ANALYSIS　OF　GENOMIC　DNA　OF　5　STRAINS　OF　TRICHINELLA　SPIRALIS　IN　CHINA

中国医学科学杂志（英文版） 1995年第3期

Updated：2024-04-10

- RESTRICTION　FRAGMENT　LENGTH　POLYMORPHISM（RFLP）　ANALYSIS　OF　GENOMIC　DNA　OF　5　STRAINS　OF　TRICHINELLA　SPIRALIS　IN　CHINA
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  中图分类号： Q78
- 纸质出版日期：1995
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[1]王虹,张月清,劳为德,吴赵永.RESTRICTION　FRAGMENT　LENGTH　POLYMORPHISM（RFLP）　ANALYSIS　OF　GENOMIC　DNA　OF　5　STRAINS　OF　TRICHINELLA　SPIRALIS　IN　CHINA[J].Chinese Medical Sciences Journal,1995(03):131-135.

RESTRICTION FRAGMENT LENGTH POLYMORPHISM（RFLP） ANALYSIS OF GENOMIC DNA OF 5 STRAINS OF TRICHINELLA SPIRALIS IN CHINA[J]. Chinese medical sciences journal, 1995, (3).
[1]王虹,张月清,劳为德,吴赵永.RESTRICTION　FRAGMENT　LENGTH　POLYMORPHISM（RFLP）　ANALYSIS　OF　GENOMIC　DNA　OF　5　STRAINS　OF　TRICHINELLA　SPIRALIS　IN　CHINA[J].Chinese Medical Sciences Journal,1995(03):131-135. DOI：

RESTRICTION FRAGMENT LENGTH POLYMORPHISM（RFLP） ANALYSIS OF GENOMIC DNA OF 5 STRAINS OF TRICHINELLA SPIRALIS IN CHINA[J]. Chinese medical sciences journal, 1995, (3). DOI：

摘要

ＲＥＳＴＲＩＣＴＩＯＮＦＲＡＧＭＥＮＴＬＥＮＧＴＨＰＯＬＹＭＯＲＰＨＩＳＭ（ＲＦＬＰ）ＡＮＡＬＹＳＩＳＯＦＧＥＮＯＭＩＣＤＮＡＯＦ５ＳＴＲＡＩＮＳＯＦＴＲＩＣＨＩＮＥＬＬＡＳＰＩＲＡＬＩＳＩＮＣＨＩＮＡＷａｎｇＨｏｎｇ（王虹）ＺｈａｎｇＹｕｅｑｉｎｇ...

Abstract

Five restriction endonucleases were used to digest genomic DNA from 5 isolates of Trichinella spiralis obtained from Changchun

Tianjin

Xian

Henan and Yunnan. All the isolates were secured from pigs except the Changchun strain which came from dog. The DNA fragments digested by endonuclease were separated by agarose gel electrophoresis. The Changchun isolate had a EcoRI band at 1. 12kb and a DraI band at 1. 97kb which were unique to this isolate. A cloned specific repetitive DNA sequence （1. 12kb） from the Changchun strain was selected to prepare a probe for the Southern blotting of EcoRI restriction DNA fragments for the 5 isolates. The 1.12kb hybridizing band did not appear except in the Changchun isolate.These results seem to indicate that there are differences between the isolates obtained from hosts in different geographical regions.

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