慢性阻塞性肺疾病伴抑郁对老年大鼠空间学习记忆能力的影响

蔡萃; 徐长青; 金华良; 厉蓓

doi:10.24920/003470

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慢性阻塞性肺疾病伴抑郁对老年大鼠空间学习记忆能力的影响

Chinese Medical Sciences Journal 2018年33卷第4期页码：260-266

论著 | Updated：2024-04-10

- 慢性阻塞性肺疾病伴抑郁对老年大鼠空间学习记忆能力的影响
- Affiliations：
  
  1 Department of Geriatrics, Hangzhou Red Cross Hospital, Hangzhou 310003, China
  2 Department of Respiratory, the Affiliated Hospital of Hangzhou Normal University,Hangzhou 330015, China
  3 Department of Respiratory, Hangzhou 310006, China
  4 Department of Geriatrics,Hangzhou First People’s Hospital, Hangzhou 310006, China;
- Author bio：
  
  * Tel: 86-0571-56109766; E-mail:caicui2003@aliyun.com
- Funds：
  
  浙江省自然科学基金;Supported by the Natural Science Foundation of Zhejiang Province(LQ12H27005)
- DOI：10.24920/003470
  中图分类号：
- 收稿日期：2018-03-09，
  
  录用日期：2018-8-27，
  
  网络出版日期：2018-01-07，
  
  纸质出版日期：2018-01-07
- Accepted：
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蔡萃, 徐长青, 金华良, 等. 慢性阻塞性肺疾病伴抑郁对老年大鼠空间学习记忆能力的影响[J]. Chinese Medical Sciences Journal, 2018,33(4):260-266.

Cai Cui, Xu Changqing, Jin Hualiang, et al. Combined Effects of Chronic Obstructive Pulmonary Disease and Depression on Spatial Memory in Old Rats[J]. Chinese medical sciences journal, 2018, 33(4): 260-266.
蔡萃, 徐长青, 金华良, 等. 慢性阻塞性肺疾病伴抑郁对老年大鼠空间学习记忆能力的影响[J]. Chinese Medical Sciences Journal, 2018,33(4):260-266. DOI： 10.24920/003470.

Cai Cui, Xu Changqing, Jin Hualiang, et al. Combined Effects of Chronic Obstructive Pulmonary Disease and Depression on Spatial Memory in Old Rats[J]. Chinese medical sciences journal, 2018, 33(4): 260-266. DOI： 10.24920/003470.

摘要

目的

老年慢性阻塞性肺疾病（Chronic obstructive pulmonary disease

COPD)患者常常合并抑郁状态

但目前对于老年人COPD伴抑郁的基础研究较少。本文探讨COPD伴抑郁对老年大鼠空间学习记忆能力的影响

将COPD、抑郁与衰老联系起来

以进一步了解老年COPD伴抑郁患者的疾病特点。

方法

将SD大鼠分为5组

分别为成年组、老年对照组、老年COPD组、老年抑郁组及老年COPD伴抑郁组。利用烟熏结合慢性轻度不可预见性应激（chronic unpredictable mild stress

CUMS)并孤养建立老年COPD伴抑郁大鼠模型。通过Morris水迷宫 (Morris water maze

MWM)试验检测大鼠的空间学习记忆能力

用黄嘌呤氧化酶法（Xanthinoxidase method）和硫代巴比妥酸反应法(TBAR)分别测定大鼠血清及海马组织中超氧化物歧化酶（SOD）的活性和丙二醛（MDA）的含量。比较各实验组与对照组各观测指标之间的差异。

结果

肺组织学、肺功能检测、敞箱实验及糖水消耗实验证实烟熏结合CUMS并孤养方法成功建立老年COPD伴抑郁大鼠模型。与老年对照组相比

老年COPD伴抑郁大鼠组在Morris水迷宫实验中到达平台的时间明显延长（LSD-

= -10.116

=0.000）

运动距离明显增加（LSD-

= -6.448

=0.000）;同时

老年COPD伴抑郁模型组中大鼠海马和血清中SOD的活性明显减少（LSD-

=2.629

=0.014; LSD-

= 2.215

=0.044)

而MDA的含量显著增加（(LSD

-t

= -2.140

=0.042; LSD

-t

=-2.070

=0.049）。

结论

慢性阻塞性肺疾病伴抑郁的老年大鼠空间学习记忆能力下降

其作用机制可能与氧化应激失衡有关。这一发现可能为临床老年COPD伴抑郁患者的治疗提供新的思路。

Abstract

Objective

To investigate the combined effects of chronic obstructive pulmonary disease (COPD) and depression on spatial memory in old rats

aiming to better understand the comorbidity of the two diseases in geriatric patients.

Methods

The SD rats were assigned into five groups: adult control group (

=6)

elderly control group (

=6)

elderly COPD group (

=6)

elderly depression group (

=6) and elderly COPD with depression group (

=6). Smoking and chronic unpredictable mild stress (CUMS) with solitary support were used to induce COPD model

depression model

respectively

and the both were applied for the comorbidity model. Learning and memory deficits were assessed by Morris water maze (MWM) test. The activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in serum and hippocampus tissue were determined by Xanthinoxidase method and Thiobarbituric acid reaction (TBAR) method

respectively.

Results

The results of pulmonary histology

lung function

open-field test and sucrose consumption demonstrated the comorbidity models of COPD and depression in elderly rats were successfully established using smoking and CUMS with solitary support. Compared with the elderly control group

the group of COPD with depression had obviously longer time of latency and longer travel distance to reach the platform in MWM test (LSD-

=-10.116

=0.000; LSD-

=-6.448

=0.000). The SOD activity in serum and hippocampus decreased significantly (LSD-

=2.629

=0.014; LSD-

=2.215

=0.044) and the MDA content in serum and hippocampus increased significantly (LSD-

=-2.140

=0.042; LSD-

=-2.070

=0.049) in elderly COPD with depression group.

Conclusions

COPD in comorbidity of depression could induce spatial memory deficit in old rats. The mechanisms might be related to the overloaded and free radical metabolic imbalance. These results suggest a potential therapeutic target for comorbidity of COPD and depression in geriatric patients.

关键词

Keywords

references

Vogelmeier CF , Criner GJ , Martinez FJ , et al . Global strategy for the diagnosis, management, and prevention of chronic obstructive lung disease 2017 Report . GOLD executive summary . Am J Respir Crit Care Med 2017 ; 195 ( 5 ): 557 - 82 . doi: 10.1164/rccm.201701-0218PP 10.1164/rccm .

Hanania NA , Sharma G , Sharafkhaneh A . COPD in the elderly patient . Semin Respir Crit Care Med 2010 ; 31 ( 5 ): 596 - 606 . doi: 10.1055/s-0030-1265900 10.1055/s-0030-1265900 . DOI: 10.1055/s-0030-1265900 http://doi.org/10.1055/s-0030-1265900 http://www.thieme-connect.de/DOI/DOI?10.1055/s-0030-1265900 http://www.thieme-connect.de/DOI/DOI?10.1055/s-0030-1265900

Blanchette CM , Berry SR , Lane SJ . Advances in chronic obstructive pulmonary disease among older adults . Curr Opin Pulm Med 2011 ; 17 ( 2 ): 84 - 9 . doi: 10.1097/MCP.0b013e32834316ff 10.1097/MCP.0b013e32834316ff . DOI: 10.1097/MCP.0b013e32834316ff http://doi.org/10.1097/MCP.0b013e32834316ff https://insights.ovid.com/crossref?an=00063198-201103000-00007 https://insights.ovid.com/crossref?an=00063198-201103000-00007

Yuan YM. Aging and COPD. Chin J Clinician 2013 ; 7(2): 478-80 . Chinese. doi: 10.3877/cma.j.issn.1674-0785.2013.02.008 10.3877/cma.j.issn.1674-0785.2013.02.008 .

Lou P , Zhu Y , Chen P . Prevalence and correlations with depression, anxiety and other features in outpatients with chronic obstructive pulmonary disease in China: a cross-sectional case control study . BMC Pulm Med 2012 ; 12 : 53 . doi: 10.1186/1471-2466-12-53 10.1186/1471-2466-12-53 . DOI: 10.1186/1471-2466-12-53 http://doi.org/10.1186/1471-2466-12-53 http://bmcpulmmed.biomedcentral.com/articles/10.1186/1471-2466-12-53 http://bmcpulmmed.biomedcentral.com/articles/10.1186/1471-2466-12-53

Cui GM , Zhu ZM , . Related factors of the influence of depressive disorder on senior patients with COPD . Chin J practical Mervous Dis 2013 ; 16 ( 20 ): 33 - 5 . Chinese. doi: 10.3969/j.issn.1673-5110.2013.20.018 10.3969/j.issn.1673-5110.2013.20.018 . http://en.cnki.com.cn/Article_en/CJFDTOTAL-HNSJ201320018.htm http://en.cnki.com.cn/Article_en/CJFDTOTAL-HNSJ201320018.htm

Russo E , Citraro R , Davoli A , et al . Ameliorating effects of aripiprazole on cognitive functions and depressive-like behavior in a genetic rat model of absence epilepsy and mild-depression comorbidity . Neuropharmacology 2013 ; 64 : 371 - 9 . doi: 10.1016/j.neuropharm.2012.06.039 10.1016/j.neuropharm.2012.06.039 . DOI: 10.1016/j.neuropharm.2012.06.039 http://doi.org/10.1016/j.neuropharm.2012.06.039 https://linkinghub.elsevier.com/retrieve/pii/S0028390812002973 https://linkinghub.elsevier.com/retrieve/pii/S0028390812002973

Fernandez JW , Grizzell JA , Philpot RM , et al . Postpartum depression in rats: differences in swim test immobility, sucrose preference and nurturing behaviors . Behav Brain Res 2014 ; 272 ( 4 ): 75 - 82 . doi: 10.1016/j.bbr.2014.06.041 10.1016/j.bbr.2014.06.041 . DOI: 10.1016/j.bbr.2014.06.041 http://doi.org/10.1016/j.bbr.2014.06.041 https://linkinghub.elsevier.com/retrieve/pii/S0166432814004240 https://linkinghub.elsevier.com/retrieve/pii/S0166432814004240

Lee B , Sur B , Cho SG , et al . Effect of Beta-Asarone on Impairment of Spatial Working Memory and Apoptosis in the Hippocampus of Rats Exposed to Chronic Corticosterone Administration . Biomol Ther (Seoul) 2015 ; 23 ( 6 ): 571 - 81 . doi: 4062/biomolther.2015.027 4062/biomolther.2015.027 . DOI: 10.4062/biomolther.2015.027 http://doi.org/10.4062/biomolther.2015.027 http://www.biomolther.org/journal/DOIx.php?id=10.4062/biomolther.2015.027 http://www.biomolther.org/journal/DOIx.php?id=10.4062/biomolther.2015.027

Ellison D , White D , Farrar FC . Aging population . Nurs Clin North Am 2015 ; 50 ( 1 ): 185 - 213 . doi: 10.1016/j.cnur.2014.10.014 10.1016/j.cnur.2014.10.014 . DOI: 10.1016/j.cnur.2014.10.014 http://doi.org/10.1016/j.cnur.2014.10.014 https://linkinghub.elsevier.com/retrieve/pii/S0029646514000887 https://linkinghub.elsevier.com/retrieve/pii/S0029646514000887

de Voogd JN , Wempe JB , Koeter GH , et al . Depressive symptoms as predictors of mortality in patients with COPD . Chest 2009 ; 135 ( 3 ): 619 - 25 . doi: 10.1378/chest.08-0078 10.1378/chest.08-0078 . DOI: 10.1378/chest.08-0078 http://doi.org/10.1378/chest.08-0078 https://linkinghub.elsevier.com/retrieve/pii/S0012369209601860 https://linkinghub.elsevier.com/retrieve/pii/S0012369209601860

Chetta A , Foresi A , Marangio E , et al . Psychological implications of respiratory health and disease . Respiration 2005 ; 72 ( 2 ): 210 - 5 . doi: 10.1159/000084056 10.1159/000084056 . DOI: 10.1159/000084056 http://doi.org/10.1159/000084056 https://www.karger.com/Article/FullText/84056 https://www.karger.com/Article/FullText/84056

Moisieieva NV , Burya LV , Kapustianskaya AA , et al . Comprehensive patterns of comorbidity: COPD and depression. Aspects of treatment . Wiad Lek 2018 ; 7 ( 3 pt 1 ): 588 - 91 . http://europepmc.org/abstract/MED/29783230 http://europepmc.org/abstract/MED/29783230

Bock K , Bendstrup E , Hilberg O , et al . Screening tools for evaluation of depression in Chronic Obstructive Pulmonary Disease (COPD). A systematic review . Eur Clin Respir 2017 ; 4 ( 1 ): 1332931 . doi: 10.1080/20018525.2017.1332931 10.1080/20018525.2017.1332931 . DOI: 10.1080/20018525.2017.1332931 http://doi.org/10.1080/20018525.2017.1332931 https://www.tandfonline.com/doi/full/10.1080/20018525.2017.1332931 https://www.tandfonline.com/doi/full/10.1080/20018525.2017.1332931

Corlateanu A , Covantev S , Mathioudakis AG , et al . Prevalence and burden of comorbidities in Chronic Obstructive Pulmonary Disease . Respir Investig 2016 ; 54 ( 6 ): 387 - 96 . doi: 10.1016 /j. resinv.2016.07.001 10.1016 /j. resinv.2016.07.001 . DOI: 10.1016/j.resinv.2016.07.001 http://doi.org/10.1016/j.resinv.2016.07.001 https://linkinghub.elsevier.com/retrieve/pii/S2212534516300703 https://linkinghub.elsevier.com/retrieve/pii/S2212534516300703

Lavrencic LM , Richardson C , Harrison SL , et al . Is there a link between cognitive reserve and cognitive function in the oldest-old? J Gerontol A Biol Sci Med 2018 ; 73 ( 4 ): 499 - 505 . doi: 10.1093/gerona/glx140 10.1093/gerona/glx140 . DOI: 10.1093/gerona/glx140 http://doi.org/10.1093/gerona/glx140 https://academic.oup.com/biomedgerontology/article/73/4/499/3999250 https://academic.oup.com/biomedgerontology/article/73/4/499/3999250

van Beers M , Janssen DJA , Gosker HR , et al . Cognitive impairment in chronic obstructive pulmonary disease: disease burden, determinants and possible future interventions . Expert Rev Respir Med 2018 ; 12 ( 12 ): 1061 - 74 . doi: 10.1080/17476348.2018.1533405 10.1080/17476348.2018.1533405 . DOI: 10.1080/17476348.2018.1533405 http://doi.org/10.1080/17476348.2018.1533405 https://www.tandfonline.com/doi/full/10.1080/17476348.2018.1533405 https://www.tandfonline.com/doi/full/10.1080/17476348.2018.1533405

Aras YG , Tunc A , Güngen BD , et al . The effects of depression, anxiety and sleep disturbances on cognitive impairment in patients with chronic obstructive pulmonary disease . Cogn Neurodyn 2017 ; 11 ( 6 ): 565 - 71 . doi: 10.1007/s11571-017-9449-x 10.1007/s11571-017-9449-x . DOI: 10.1007/s11571-017-9449-x http://doi.org/10.1007/s11571-017-9449-x http://link.springer.com/10.1007/s11571-017-9449-x http://link.springer.com/10.1007/s11571-017-9449-x

Burger C . Region-specific genetic alternations in the aging hippocampus: implications for cognitive aging . Front Aging Neurosci 2010 ; 2 : 140 . doi: 10.3389/fnagi.2010.00140 10.3389/fnagi.2010.00140 .

Stockmeier CA , Mahajan GJ , Konick LC , et al . Cellular changes in the postmortem hippocampus in major depression . Biol Psychiatry 2004 ; 56 ( 9 ): 640 - 50 . doi: 10.1016/j.biopsych.2004.08.022 10.1016/j.biopsych.2004.08.022 . DOI: 10.1016/j.biopsych.2004.08.022 http://doi.org/10.1016/j.biopsych.2004.08.022 http://linkinghub.elsevier.com/retrieve/pii/S0006322304009369 http://linkinghub.elsevier.com/retrieve/pii/S0006322304009369

Zhu J , Mu X , Zeng J , et al . Ginsenoside Rg1 prevents cognitive impairment and hipppcamps senescence in a rat model of D-Galactose-induced Aging . PLoS One 2014 ; 9 ( 6 ): e101291 . doi: 10.1371/journal.pone.0101291 10.1371/journal.pone.0101291 . DOI: 10.1371/journal.pone.0101291 http://doi.org/10.1371/journal.pone.0101291 https://dx.plos.org/10.1371/journal.pone.0101291 https://dx.plos.org/10.1371/journal.pone.0101291

Martínez-Aguilar , NE , Vargas-Camaño ME , Hernández-Pliego RR ,et al. Immunopathology of chronic obstructive pulmonary disease . Rev Alerg Mex 2017 ; 64 ( 3 ): 327 - 46 . doi: 10.29262/ram.v64i3.263 10.29262/ram.v64i3.263 . http://www.ncbi.nlm.nih.gov/pubmed/29046030 http://www.ncbi.nlm.nih.gov/pubmed/29046030

Diniz BS , Mendes-Silva AP , Silva LB , et al . Oxidative stress markers imbalance in late-life depression . J Psychiatr Res 2018 ; 102 : 29 - 33 . doi: 10.1016/j.jpsychires.2018.02.023 10.1016/j.jpsychires.2018.02.023 . DOI: 10.1016/j.jpsychires.2018.02.023 http://doi.org/10.1016/j.jpsychires.2018.02.023 https://linkinghub.elsevier.com/retrieve/pii/S0022395617313365 https://linkinghub.elsevier.com/retrieve/pii/S0022395617313365

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