纵向测量大鼠非动脉炎性前部缺血性视神经病变模型中后部视乳头血流变化的研究

马瑾; 陈婷; 王一玮; 赵潺; 李东辉; 王萌; 干霖洋; 钟勇

doi:10.24920/003477

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纵向测量大鼠非动脉炎性前部缺血性视神经病变模型中后部视乳头血流变化的研究

Chinese Medical Sciences Journal 2018年33卷第4期页码：252-259

论著 | Updated：2024-04-10

- 纵向测量大鼠非动脉炎性前部缺血性视神经病变模型中后部视乳头血流变化的研究
- Affiliations：
  
  1.中国医学科学院北京协和医学院北京协和医院眼科
  2.1 中国医学科学院北京协和医学院北京协和医院眼科
- Author bio：
- Funds：
  
  国家自然科学基金;Supported by the National Natural Science Foundation(81670854)
- DOI：10.24920/003477
  中图分类号：
- 收稿日期：2018-05-07，
  
  录用日期：2018-9-3，
  
  网络出版日期：2018-12-10，
  
  纸质出版日期：2018-12-10
- Accepted：
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马瑾, 陈婷, 王一玮, 等. 纵向测量大鼠非动脉炎性前部缺血性视神经病变模型中后部视乳头血流变化的研究[J]. Chinese Medical Sciences Journal, 2018,33(4):252-259.

Ma Jin, Chen Ting, Wang Yiwei, et al. Longitudinal Measurement of Hemodynamic Changes within the Posterior Optic Nerve Head in Rodent Nonarteritic Anterior Ischemic Optic Neuropathy[J]. Chinese medical sciences journal, 2018, 33(4): 252-259.
马瑾, 陈婷, 王一玮, 等. 纵向测量大鼠非动脉炎性前部缺血性视神经病变模型中后部视乳头血流变化的研究[J]. Chinese Medical Sciences Journal, 2018,33(4):252-259. DOI： 10.24920/003477.

Ma Jin, Chen Ting, Wang Yiwei, et al. Longitudinal Measurement of Hemodynamic Changes within the Posterior Optic Nerve Head in Rodent Nonarteritic Anterior Ischemic Optic Neuropathy[J]. Chinese medical sciences journal, 2018, 33(4): 252-259. DOI： 10.24920/003477.

摘要

目的

活体评估大鼠非动脉炎性前部缺血性视神经病变(Rat nonarteriticanterior ischemic optic neuropathy

rNAION）模型后部视乳头血流灌注的动态变化。

方法

采用SD 鼠孟加拉玫瑰红股静脉注射和氩绿激光法制作rNAION模型。通过眼底彩照和荧光素钠血管造影（Fundus fluorescein angiography

FFA）分别评估造模后视神经90天内形态变化和3小时内的血供变化

采用组织形态学来判断模型是否成功建立

采用激光多普勒血流仪（Laser doppler flowmetry

LDF）在造模后第3天、7天、14天、21天和40天测量后部视乳头血流动力学的动态变化。统计分析采用单因素方差分析

Student’s t检验以及Bonferroni校正法进行多重比较。

结果

在rNAION模型中观察到视乳头水肿、消退与视盘苍白密切相关。FFA显示早期视盘低荧光

晚期高荧光。组织学研究显示视乳头组织水肿、疏松

视网膜神经节细胞和神经纤维大量丢失

大量神经胶质增生。与正常大鼠相比

造模后大鼠各观察时间点血流动力学参数值均明显降低（

=175.06

0.0001）

以在造模后14天以内下降最为显著(All

0.01)。

结论

rNAION模型病程中后部视乳头血流灌注持续降低

且在疾病发生14天前变化最为明显。该结果为探明rNAION模型中血流动态变化提供重要参考信息。

Abstract

Objective

To assess the

in vivo

dynamic blood flow features of posterior optic nerve head (ONH) in rat model of nonarteritic anterior ischemic optic neuropathy (rNAION).

Methods

rNAION was established with Rose Bengal and argon green laser in Sprague-Dawley rats. Fundus photography and fundus fluorescein angiography (FFA) were performed to assess the dynamic changes of optic disc in morphology in 90 days and in blood perfusion in 3 hours after the induction of disease. Histological examinations were performed to evaluate the success of modeling. The dynamic blood flow kinetics of posterior ONH in rNAION were measured by Laser Doppler Flowmetry (LDF) on the day 3

and 40 after the disease induction. One-way ANOVA

Student’s

-test and Bonferroni adjustment were used for multiple comparisons of kinetic measurements of blood flow.

Results

Optic disc edema and subsequent resolution associated with the development of optic disc pallor were observed in rNAION. FFA showed that the optic disc was hypofluorescence in the early phase and hyperfluorescence in the late phase. Histological studies suggested edema and loosened tissues of ONH

loss of retinal ganglion cells (RGCs)

optic nerve substance and gliosis. Compared to the naive rats

the blood flow kinetics of posterior ONH in rNAION significant reduced at each time point after modeling (

=175.06

0.0001). The reductions were specifically remarkable in 14 days after the disease induction (All

0.01).

Conclusions

Continuous blood perfusion reduction was found in rNAION

with significant alteration in 14 days after disease induction. Our results provided important information for understanding the hemodynamic changes in rNAION.

关键词

Keywords

references

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