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亚甲基四氢叶酸还原酶基因C677T多态性与中国2型糖尿病患者冠心病风险增加相关
- Affiliations：
1Department of Clinical Pharmacy, The First Affiliated Hospital of Shandong First Medical University, Jinan 250014, China
2School of Pharmaceutical Sciences, Shandong First Medical University (Shandong Academy of Medical Sciences) Tai’an 271000, Shandong, China
- Author bio：
*Tel: 86-13791126823, E-mail: prof_liyan@126.com
- Funds：National Key Development Plan for Precision Medicine Research(2017YFC0910004);Jinan Science Project(201602171)
DOI：10.24920/003792
中图分类号：收稿日期：2020-08-18，
网络出版日期：2020-12-01，
纸质出版日期：2021-06-30
- Accepted：
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吴坤荣, 张恕芳, 关紫菀, 等. 亚甲基四氢叶酸还原酶基因C677T多态性与中国2型糖尿病患者冠心病风险增加相关[J]. 中国医学科学杂志（英文版）, 2021,36(2):103-109.
Wu Kunrong, Zhang Shufang, Guan Ziwan, et al. Methylenetetrahydrofolate Reductase Gene Polymorphism C677T is Associated with Increased Risk of Coronary Heart Disease in Chinese Type 2 Diabetic Patients[J]. Chinese medical sciences journal, 2021, 36(2): 103-109.
吴坤荣, 张恕芳, 关紫菀, 等. 亚甲基四氢叶酸还原酶基因C677T多态性与中国2型糖尿病患者冠心病风险增加相关[J]. 中国医学科学杂志（英文版）, 2021,36(2):103-109. DOI： 10.24920/003792.
Wu Kunrong, Zhang Shufang, Guan Ziwan, et al. Methylenetetrahydrofolate Reductase Gene Polymorphism C677T is Associated with Increased Risk of Coronary Heart Disease in Chinese Type 2 Diabetic Patients[J]. Chinese medical sciences journal, 2021, 36(2): 103-109. DOI： 10.24920/003792.
摘要
目的
长期血糖控制不佳引起的慢性心血管疾病是2型糖尿病患者主要的死亡原因。研究表明：亚甲基四氢叶酸还原酶基因（methylenetetrahydrofolate reductase gene
MTHFR
）多态性可能会影响2型糖尿病患者冠心病的发生。本研究旨在评估
MTHFR
C677T和A1298C基因突变是否与2型糖尿病患者冠心病的发生风险相关。
方法
本研究一共纳入197例2型糖尿病受试者
其中95例受试者合并冠心病。采用双脱氧链终止法对
MTHFR
C677T和A1298C进行基因分型
并分析比较合并冠心病与不合并冠心病受试者等位基因频率的差异。
结果
合并冠心病2型糖尿病受试者的677T等位基因频率明显高于不合并冠心病的受试者（
P
=0.011）。单倍型频率在合并冠心病与不合并冠心病的2型糖尿病受试者之间无显著的差异。在同型半胱氨酸水平较低的糖尿病受试者中（≤15μmol/L）
携带677T等位基因的受试者发生冠心病风险更高（
P
=0.006）
而在同型半胱氨酸水平较高的糖尿病受试者中（
>
15μmol/L）
MTHFR
基因多态性对冠心病的发生风险无影响（
P
=0.491）。
结论
MTHFR
C677T基因多态性与糖尿病患者发生冠心病的风险相关
对于具有正常同型半胱氨酸水平的中国糖尿病人群
其可作为预测冠心病风险的有效标志物。
Abstract
Objective
Chronic cardiovascular diseases induced by long-term poor blood glucose control are the main cause of death in patients with type 2 diabetes mellitus (T2DM). Previous researches report that methylenetetrahydrofolate reductase gene (
MTHFR
) polymorphisms might influence the occurrence of coronary heart disease (CHD) in T2DM patients. The purpose of this study was to evaluate whether
MTHFR
C677T and A1298C mutations are associated with the risk of CHD in T2DM patients.
Methods
A total of 197 subjects with T2DM were studied
of which 95 patients with CHD. The genotypes of
MTHFR
C677T and A1298C were analyzed by using dideoxy chain-termination method
and compared between patients with CHD and those without CHD.
Results
We found that the frequency of the 677T allele was significantly higher in T2DM patients with CHD than those without CHD (
P
=0.011). However
there was no significant difference in any of the examined haplotypes between T2DM patients with and without CHD. Furthermore
the 677T allele was associated with a higher risk of CHD development in diabetic patients with lower homocysteine (Hcy) levels (≤15 μmol/L) (
P
=0.006)
while no effect of
MTHFR
gene polymorphism on the incidence of CHD was found in patients with higher Hcy levels (
>
15 μmol/L) (
P
=0.491).
Conclusion
The
MTHFR
C677T gene polymorphism is associated with the risk of CHD of diabetic patients and could be used as an effective marker for CHD in Chinese diabetic populations with normal Hcy levels.
关键词
Keywords
references
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