<italic style="font-style: italic">TOP2A</italic>基因对预测肝细胞癌预后的作用

陆进; 安韶光; 马俊杰; 杨月; 张磊; 俞鹏; 陶恒; 陈云帆; 张浩轩

doi:10.24920/004006

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TOP2A基因对预测肝细胞癌预后的作用

中国医学科学杂志（英文版） 2022年37卷第4期页码：331-339

Original article | Updated：2024-04-10

- TOP2A基因对预测肝细胞癌预后的作用
- Affiliations：
  
  1. 1School of Basic Medicine, Bengbu Medical College, Bengbu, Anhui 233030, China
  2. 2Anhui Key Laboratory of Computational Medicine and Intelligent Health, Bengbu Medical College, Bengbu, Anhui 233030, China
  3. 3Clinical Medical College, Bengbu Medical College, Bengbu, Anhui 233030, China
  4. 4Department of Otolaryngology, First Affiliated Hospital, Bengbu Medical College, Bengbu, Anhui 233030, China
  5. 5Department of Surgical Oncology, Second Affiliated Hospital, Bengbu Medical College, Bengbu, Anhui 233030, China
- Author bio：
  
  * 86-552-3175286, E-mail: 0100197@bbmc.edu.cn
- Funds：
- DOI：10.24920/004006
  中图分类号：
- 收稿日期：2021-09-22，
  
  录用日期：2021-12-10，
  
  网络出版日期：2022-10-04，
  
  纸质出版日期：2022-12-31
- Accepted：
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陆进, 安韶光, 马俊杰, 等. TOP2A基因对预测肝细胞癌预后的作用[J]. 中国医学科学杂志（英文版）, 2022,37(4):331-339.

Jin Lu, Shaoguang An, Junjie Ma, et al. Topoisomerase Ⅱα Gene as a Marker for Prognostic Prediction of Hepatocellular Carcinoma: A Bioinformatics Analysis[J]. Chinese medical sciences journal, 2022, 37(4): 331-339.
陆进, 安韶光, 马俊杰, 等. TOP2A基因对预测肝细胞癌预后的作用[J]. 中国医学科学杂志（英文版）, 2022,37(4):331-339. DOI： 10.24920/004006.

Jin Lu, Shaoguang An, Junjie Ma, et al. Topoisomerase Ⅱα Gene as a Marker for Prognostic Prediction of Hepatocellular Carcinoma: A Bioinformatics Analysis[J]. Chinese medical sciences journal, 2022, 37(4): 331-339. DOI： 10.24920/004006.

摘要

目的

研究拓扑异构酶Ⅱα(

topoisomerase II α

，

TOP2α

)基因在肝细胞癌（hepatocellular carcinoma，HCC）中的表达及其在预测HCC患者预后中的作用。

方法

我们利用UALCAN、HCCDB、cBioPortal和Enrichr数据库中的HCC相关数据集，分析

TOP2α

及其共表达基因在HCC组织中的表达和突变情况

以及

TOP2α

及其共表达基因的GO功能和KEGG通路富集情况。利用TIMER数据库分析HCC组织中浸润的免疫细胞水平。采过

Kaplan-Meier

分析

TOP2α

及其共同表达基因与浸润的免疫细胞对HCC患者生存的影响。

结果

TOP2α

及其共同表达基因在HCC组织中高表达（

0.001），并与HCC患者的总生存期缩短有关（

0.0001）。

TOP2α

及其共表达基因主要参与细胞有丝分裂和增殖以及细胞周期通路过程（ID：hsa04110，

= 0.001945）。

TOP2α

及其共同表达基因的突变与HCC患者的总生存期（

= 0.0247）和无病生存期（

= 0.0265）的缩短有关。

TOP2α

与HCC肿瘤组织中B细胞（

= 0.459

0.01）、CD8

T细胞（

= 0.312

0.01）、CD4

T细胞（

= 0.370

0.01）、巨噬细胞（

= 0.459

0.01）、中性粒细胞（

= 0.405

0.01）和树突状细胞（

= 0.473

0.01）的浸润量呈正相关。CD8

T细胞的浸润能显著延长HCC患者3年和5年生存期（

均

0.05），而CD4

T细胞的浸润能显著缩短HCC患者的3、5和10年生存期（

均

0.05）。

结论

TOP2α

可能是一种癌基因，与HCC患者预后不良有关，其可能成为预测肝细胞癌患者预后的生物标志物。

Abstract

Objective

To investigate the expression of

topoisomerase

Ⅱ

(

TOP2α

) in hepatocellular carcinoma (HCC) and its role in predicting prognosis of HCC patients.

Methods

We used HCC-related datasets in UALCAN

HCCDB

and cBioPortal databases to analyze the expression and mutation of

TOP2α

and its co-expressed genes in HCC tissues. GO function and KEGG pathway enrichment of

TOP2α

and its co-expressed genes were identified. The TIMER database was used to analyze infiltration levels of immune cells in HCC. The impacts of

TOP2α

and its co-expression genes and the infiltrated immune cells on the survival of HCC patients were assayed by

Kaplan-Meier

plotter analysis.

Results

TOP2α

and its co-expression genes were highly expressed in HCC (

0.001) and detrimental to overall survival of HCC patients (

0.001).

TOP2α

and its co-expression genes were mainly involved in cell mitosis and proliferation

and cell cycle pathway (ID: hsa04110

= 0.001945).

TOP2α

and its co-expression genes were mutated in HCC and the mutations were significantly detrimental to overall survival (

= 0.0247) and disease-free survival (

= 0.0265) of HCC patients. High

TOP2α

expression was positively correlated with the infiltration of B cell (

= 0.459

0.01)

CD8

T cell (

= 0.312

0.01)

CD4

T cell (

= 0.370

0.01)

macrophage (

= 0.459

0.01)

neutrophil (

= 0.405

0.01)

and dendritic cell (

= 0.473

0.01) in HCC. The CD8

T cell infiltration significantly prolonged the 3- and 5-year survival of HCC patients (all

0.05)

and CD4

T cell infiltration significantly shortened the 3-

and 10-year survival of HCC patients (all

0.05).

Conclusion

TOP2α

may be an oncogene

which was associated with poor prognosis of HCC patients and could be used as a biomarker for the prognostic prediction of HCC.

关键词

Keywords

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TOP2A基因对预测肝细胞癌预后的作用

DOI：10.24920/004006

摘要

Abstract

关键词

Keywords

references