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1990 Year 1 Issue
- Abstract：<正> Between August 1976 and May 1988,43 patients had their heart valve prostheses replaced.The total mortality was as high as 25.6%,but the operative mortality was reduced to 6.25%in the last 16 cases.The indications,main risk factors and surgical techniques are discussed.4|7|0Updated：2024-04-10
- Abstract：<正> Antibodies raised against rabbit sperm membrane protein（rSMP-B）interacted with a rattestis cytosolic protein with an estimated molecular weight of 70 kD.The clone expressingrSMP-B was isolated by epitope selection from a rat testis,λgt11 vector cDNA library,An Eco Rl-eut 2.0 kb cDNA fragment encoding rSMP-B was identified.The cDNA fragment（RSD-1）was isolated,subcloned and restriction mapped.Dot hybridization analysis of mRNAprepared from human,rabbit and rat testes showed that the gene encoding rSMP-B is ex-pressed in the testes of all three mammals.5|17|0Updated：2024-04-10
- Abstract：<正> Passive immunization of female mice by the intraperitoneal administration of ascitic fluidscontaining monoclonal antisperm antibodies blocked the development of mouse zygotes tomorulae and blastocysts when cultured in vitro.Radioiodinated YWK-I monoclonal anti-bodies were prepared and administered intraperitoneally to Jemale mice.Significant radioac-tivity was found in washes of the oviductal lumens.The results suggest that antispermantibodies block fertilization and may have a deleterious effect on early developing embryos.4|14|2Updated：2024-04-10
- Abstract：<正> The changes of carbamyl phosphate synthetase I（CPS 1）in diethylnitrosamine-（DEN）-inducedenzyme-altered liver cells were studied by means of immunohistochemical（PAP）and in situcDNA-mRNA hybridization methods.The experimental rats were treated with DEN,2-acetylaminofluorene（2-AAF）and 2/3 hepatectomy according to Solt-Farber’s protocol andwere further promoted by oral daily administration of 0.05% phenobarbital in drinking water.The results showed that the average number of lesions showing abnormal expression of CPS1 was relatively constant over the course of the experiment（8 months）,while the numberof normally expressing lesions gradually decreased.The former lesions were also largerin volume than the latter ones.We conclude that in DEN-initiated lesions the abnormallyexpressed CPS 1 lesions may grow continuously,thus leading to the formation of largernodules.We also suspect that some of these lesions have increased tendencies to developinto tumors.4|11|0Updated：2024-04-10
- Abstract：<正> It was found that the DNA-damaging agents N-methyl-N’-nitro-N-nitrosoguanidine（MNNG）,methyl-methanesulphonate（MMS）and 4-nitroquinoline-N-oxide（4NQO）could stimulate ADP-ribosyl transferase（ADPRT）activity and reduce the cellular NAD content in a dose-dependent way.The reduction of NAD after DNA damage could be partially or completelyprevented by ADPRT inhibitors,3-aminobenzamide or nicotinamide,which showed noinfluence on reduction of NAD induced by metabolic blocking agents.Therefore,a simpleand specific method to detect DNA-damaging mutagens by measuring ADPRT-mediateddecrease of cellular NAD content was explored.Using β-naphthofiavone,a mixed functionoxygenase inducer,together with induced or uninduced human amnion FL cells,it was foundthat aflatoxin B1,benzo（a）pyrene,2-acetylaminofluorene,9,10-dimethylanthracene andethylcarbamate could induce the ADPRT-mediated decrease of cellular NAD content,while4-acetylaminofluorene,anthracene,isopropyl-N-（3-chlorophenyl）-carbamate,β-propiolactone,γ-butyrolactone,cyclophosphamide and safrol could not.The results indicate that this isa cheap and specific method to detect DNA damage caused by chemical carcinogens/mutagenswith a spccificity approaching that of the unscheduled DNA synthesis assay.4|14|0Updated：2024-04-10
- Abstract：<正> Gossypol-induced alterations of aminophospholipid composition in human sperm wereobserved and the effects of some factors on these alterations were investigated.The altera-tions of aminophospholipid composition of human sperm induced by gossypoI included aprogressive decrease in the levels of phosphatidylethanolamine（PE）and phosphatidylserine（PS）when gossypol concentrations ranged from 5-500μmol/L,and a progressive increase inthe level of（LPE）at lower gossypol concentrations（5-50μmol/L）.The conversion of PEinto lysophosphatidylethanolamine（LPE）was strongly enhanced by Ca2+and inhibitedby 0.5mmol/L EDTA,while PMSF,NEM,iodoacetamide and Zn2+had no effect.Thisconversion was weaker in sperm with stripped surface proteins than in normal sperm.In addition,three surface proteins（MW 80,60 and 40 kD）were fixed on the plasmamembrane by gossypol treatment.The significance of gossypol action and the possibilitythat sperm PLA2, PE and.some surface proteins might play an important role in the physio-logical acrosome reaetion of human sperm,as well as in oocyte penetration,are discussed.4|6|0Updated：2024-04-10
- Abstract：<正> The migratory pattern and attrition of75Se-labelled S.mansoni challenge cercariae in CF1mice immunized 0,1,3, or 5 times with 12kR-or 48kR-irradiated cercariae were investigatedby compressed organ autoradiography.Mice were percutaneously inoculated with 500-1000 cer-cariae for each immunization and with a known count of approximately 90 cercariae at adifferent site for challenge.The skin,lungs,liver,other organs,and catrcass were processedfor autoradiography.Hepatic and mesenteric perfusions for worm collection were alsosampled from day 19 through 34.In naive tnice,25% of challenge cercariae died in theskin.Skin attrition of challenge cercariae in mice immunized with 12kR-irradiated cercariaewas 43% with 1 immunization,54% with 3,and 58% with 5,whereas in mice immunized with48kR-irradiated cercariae,skin,attrition was48% with 1,63% with 3,and 76% with 5 immunni-zations.In all immunized mice,a high percentage of migrating sehistosomula died in the skinwithin 24 hours of a challenge infection.Utilizing autoradiography,it was found that thesilver foci created by single labelled worms were difficult to differentiate from those of pairedworms.This study documents the induction of acquired resistance against a challenge infectionin the skin by vaccination with highly irradiated cercariae,with the degree of attemtation ofimmunizing cercariae and the number of immunizations being closely related to the attritiotn.rate in the skin.Finally,we discuss the contradictory views concerning the site of attritiotn ofchallenge cercariae.4|7|0Updated：2024-04-10
- Abstract：<正> The feasibility and effects of a 3-year treatment using rifampicin（RFP）,clofazimine（B663）and dapsone（DDS）in multibacillary leprosy patients in Yangzhou Prefecture and DongtaiCounty（1983-1986）are reported.Among 591 active multibaeillary leprosy patients in thetwo areas,569 （96.30%）were treated with this regimen.of 303 cases available for analysis,196（64.7%）cases showed negative skin smears and clinical inactivity.The rest showeddifferent degrees of improvement.The average reduction of BI was 0.78.The intensityand frequency of ENL and neuritis decreased markedly with treatment.The main side-effects were pigmentation and ichthyosiform changes of the skin,but these did not influencetreatment.4|8|0Updated：2024-04-10
- Abstract：<正> Donor rat parathyroid gland（PTG）was first transplanted into the nude mouse interim hostsystem.After 100 days the PTG was re-transplanted into eligible recipients.The resultsshow that mean survival time of recipient rats may be remarkably prolonged.4|6|0Updated：2024-04-10
- Abstract：<正> During the course of malignant transformation of mammalian cells in vitro,a regular varia-tion in chromosome number and structure is usually found.In order to elucidate the rela-tionship between chromosomal changes and maligannt expression,we isolated fire clonesfrom a malignant transformed Syrian hamster fibroblast cell line and analyzed their biologicalcharacteristics,as well as chromosomal changes.A positive correlation betweetn chromosomeNo 15 monosomy and the transformed and malignant phenotype was observed.We suggestthat a suppression gene may be located on chromosome No 15,and its deletion or the lossof the chromosome may result in expression of the malignant phenotype.4|9|0Updated：2024-04-10
- Abstract：<正> The effects of nimodipine on platelet aggregation and arachidonic acid（AA）metabolismwere studied in order to explore its effect on patients with thrombosis or cardiovas-cular disease.The results indicate that nimodipine（50-350μmol/L）significantly inhibitsplatelet aggregation induced by ADP,AA,and ionophore A23187 in a dose dependentmanner.The inhibitory effects induced by ionophore A23187 could be partially antagonizedby calcium（1 mmol/L）.When the substrate was AA and the enzyme was supplied bypig lung microsomes,nimodipine（50-400μmol/L）significantly reduced the generation ofTXB2 and 6-keto-PGF1a in parallel.When the substrate was prostaglandin endoperoxide,however,the levels of TXB2 and 6-keto-PGF1awere not significantly altered in the sameconcentration range.The results suggest that nimodipine is a cyclooxygenase inhibitor,andits ability to inhibit platelet aggregation is related to its calcium blocking effect.4|16|0Updated：2024-04-10
- Abstract：<正> Experimental study both in vitro and in vivotogether with clinical trials showed that LAKcells have antitumor and antimetastatic effects（1-5）and that these effects are closely related tothe number of LAK cells transferred and the ad-ministration of rIL-2（1,6-8）.Usually,autologousPBL’s are used as the source of LAK precursorsin the adoptive immunotherapy of cancer patients.But this not only puts an added burden on thecancer patient,it can cause serious side effectsas well（9）.Although TIL’s may provide a solu-tion to this problem（10,11）,their isolation fromsolid tumors is complex and consumes many rea-gents.We have reported that the isolation oflymphocytes from malignant ascites or from ma-lignant pleural effusions is not only simple and4|18|0Updated：2024-04-10
- Abstract：<正> Left atrial myxoma is the most common pri-mary tumor in the heart（1）.The diagnosis ofatrial myxoma is rarely made on clinical groundsalone.This is particularly true in the elderly,asmost symptoms are easily attributed to senility.We describe in this report an 81-year-old Chinesewho was found to have a/eft atrial myxoma andwas successfully treated by surgery.4|5|0Updated：2024-04-10
- Abstract：<正> A Chinese woman of blood group B,D-and her husband of blood group AB,CCDeewere examined.The woman had not been transfused before.Their first two babiesdied.Anti-Hro and anti-e were found in the mother’s serum.During her third pregnancy,the titer of antibodies went up quickly,approximately one titer per month.After 36 weeksof pregnancy,the baby was delivered by Caesarean section.The cord blood Hb was 88g/L,his red blood cell count 2.7×1012/L,and total biIirubin 114.6 mol/L.The baby was ofblood group AB,and CDe-D-genotype.Exchangetransfusion was begun 2.5 hours afterbirth.O,ccDEE washed red cells together with group AB plasma were used.Two dayslater,7Oml washed O,ccDEE concentrated red cells were administered.The baby is aliveand in good health.4|9|0Updated：2024-04-10
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