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1997 Year 2 Issue
- Abstract：<正> Objectives. To determine overexpression of p53, EGFR, c-erbB-2 and c-erbB-3 in endometrioid carcinoma of the ovary and to evaluate the prognostic significance of these results, especially, coexisting overexpression of p53 and one of the member of type I growth factor receptor family.Methods. Overexpressions of the p53, EGFR, c-erbB-2 and c-erbB-3 protein were studied by im-munohistochemistry in paraffin-embedded tumor tissue from 28 patients with endometrioid carcinoma of the ovary.Results. 11 （39.3%）, 13 （46. 4%）, and 14 （50. 0%） were stained positively with p53, c-erbB-2 and c-erbB-3 monoclonal antibodies. 13 （46- 4%） was stained positively with EGFR polyclonal antibody. There were no relationship between p53, EGFR, C-erbB-2, c-erbB-3 and histologic grade, lymph node metastasis. The percentage of tumors with over expression of p53, EGFR, C-erbB-2 and c-erbB-3 was higher in those with stage Ⅱ-Ⅲ tumors compared with those with stage Ⅰ , in patients with residual tumor after initial surgery compared w4|33|3Updated：2024-04-10
- Abstract：<正> The biological activity of adriamycin was investigated in human breast carcinoma （HBC） cells. Adri-amycin inhibited the growth of a number of HBC cell lines and induced G1 arrest followed by apoptosis. In MCF-7 cells that harbor wild-type p53, adriamycin-induced G1 arrest and apoptosis was accompanied by p53-independent regulation of WAF1/CIP1 as well as bax mRNA levels. In MDA-MB-231 cells which possess a mutant p53, adriamycin-induced G1 arrest and apoptosis was also associated with a cpncomitant up-regulation of WAF1/CIP1 mRNA while these cells did not express bax or bcl-2 messages. Thus, adriamycin induces G1 arrest and apoptosis via a unique pathway which appears to involve activation of downstream effectors of p53-independent manner.4|20|0Updated：2024-04-10
- Abstract：<正> The acute-phase response to infection is associated with alteration in dynamics of many trace elements （TE）, particularly iron （Fe）, zinc（Zn）, copper（Cu） and selenium （Se）. It has been suggested that the alteration of TE may represent an important defense mechanism, which has been underlined by the term"nutritional immunity". The goal of this study was to measure the serum Fe, Zn, Cu and Se during infectious processes, which might be given the references of sup-4|6|0Updated：2024-04-10
- Abstract：<正> A malignant transformed mammary epithelial cell line （11A1） was transfected with liposome encapsulated eukaryotic expression plasmid pCMV-neo-RB, yielding 4 constant clones which have obvious pheno-typic reversion changes, and named 11A1-R1-R4 respectively. Further experiments showed that the 11A1-R1 behaved like normal epithelial cells in both morphological and biological characteristics, with decreased clonogenicity in solid argar medium as well as decreased tumorigenicity. Northern blot hybridization showed increased expression of RB gene and decreased expression of c-myc gene in 11A1-R1, 11A1-R2 cells compared to 11A1 cells. This was an ideal phenotypic reversion model for epithelial transformed cell line and demonstrated that the RB gene can reexpress and suppress malignant phenotype in RB inactive cells.4|13|0Updated：2024-04-10
- Abstract：<正> In this study, the human breast cancer-bearing nude mice model has been established and the radioim-munoimaging was carried out by using human anti-human monoclonal antibodies. The results showed that the breast tumor-bearing nude mice received 131I-McAb-CM-l had a clear image of the xenograft during 4 to 6 days after injection and at the same time T/NT all over 1. 0. The highest T/NT reached 7.1. It demonstrates that McAb-CM-1 can specially combine with breast cancer tissues and hopefully it could be used clinically to improve accurate rate of the early breast cancer diagnosis.4|13|0Updated：2024-04-10
- Abstract：<正> The serological assays based on antibodies to phenolic glycolipid-Ⅰ （anti-PGL-Ⅰ） have been widely employed in the studies of exposure to M. leprae in populations. A comparative investigation on seroepidemiology of leprosy based on ELISA using PGL-Ⅰ as an antigen was carried out in Wenshan Prefecture of Yunnan Province, one of the leprosy high-endemic areas in China. A total of 2453 serum specimens from community population （n=1602） and household contacts of leprosy （n=851） were examined for presence of anti-PGL-Ⅰ4|6|0Updated：2024-04-10
- Abstract：<正> An inspection of the φ-ψ angle distribution strongly suggests that protein folding is highly constrained. A number of researchers have even suggested that a relatively small set of discrete φ-ψ regions might be sufficient to describe most protein conformation. The total of 541 tight turns from 101 non-identical proteins were extracted form Brookhaven DataBank. The dihedral values of tight turns were scattered into the seven regions on the Ramachandran plot. These seven regions were called A1, A2, B1, B2, B22, T1 and T2. A1 and A2 are the traditional a-helix regions, B1, B2 and B22 the β-strand regions, Tl and T2 the β-turn regions. The A2 and T2 regions were not defined as "discrete" or single points but rather as one dimensional extended states. Based on the geometry of the two central residues of the tight turns, the new classification of β-turn was defined. This classification of the majority of β-turns fell into only six of the possible forty nine region combinations and were identifiable wi6|6|0Updated：2024-04-10
- Abstract：<正> In order to clarify the pathogenesis and rules of high altitude hemorrhagic shock, the relationship between the red cell hemorrheologic change and its immune function in early hemorrhagic shock rabbits under hypoxia was observed, so as to explore the pathogenesis of high altitude hemorrhagic shock furtherly. Fortyfive male grey rabbits were used in the experiment and devided them randomly into 3 groups: control group,4|8|2Updated：2024-04-10
- Abstract：<正> The present study observed the mutual benefit role of liver and pancreas in combined hepaticopan-creatic transplantation in rats. The results indicated that pancreas, when transplanted with liver, could survive for a significant long time （13. 4±1.01 days） than it transplanted alone （9. 2±1.14 days） （P< 0. 05, t test）. The interstitial rejection was mild and its rejection grade was significantly different from that of pancreas transplanted alone （P<0. 05, X2 test）. The liver, when transplanted with pancreas, regenerated with strong competence and contact structure morphologically compared with liver transplanted alone. We think that pancreas could be immunologically protected against rejection and liver can be nutri-tionalized by pancreas in combined liver and pancreas transplantation.4|16|0Updated：2024-04-10
- Abstract：<正> The changes in airway space following mandibular setback using sagittal split osteotomy and rigid internal fixation were studied in 28 Japanese patients with mandibular prognathism. The correlation between the amount of mandibular setback and airway space changes,as well as the correlation between the amount of airway space changes and relapse of pognion point during the following-up period were also studied. Lateral cephalometric radiographs were taken immediately before and shortly after surgery as well as 5 to 12 months postoperation. The cephalometric evaluations of airway space width and area were based on stable craniofacial landmarks. The mean setback of mandible was 8.3 mm in the right side,and 8. 2 mm in the left side. The mean amount of relapse of pogonion point during the following-up period was 0. 52 mm horizontally,and 0. 92 mm vertically. A significant decrease in airway space width and area,especially in the lower part of airway space was found following mandibular setback shortly after s4|15|0Updated：2024-04-10
- Abstract：<正> The ligamentum flavum, which is composed of a large number of elastic fibers, is of great importance for the maintenance of the normal functions of spinal physiology. However the histologic characteristics of the ligamentum flavum, esecially its ultrustructure, have not been well documented. In this study, ligamentum flavum specimens were obtained by operation from four young males with lumbar spine fracture or tumours.4|11|0Updated：2024-04-10
- Abstract：<正> The present study evaluated the effect of dl-3-n-butylphthalide（NBP） ,a novel brain protective agent, on brain edema in rats following focal ischemia. Edema was induced by occluding the right middle cerebral artery （MCAO）.producing permanent focal ischemia in the right cerebral hemisphere,which developed ip-silateral brain edema reproducibly. Edema was assessed 24 h after MCA occlusion by determining the brain water content from wet and dry weight measurements,and the sodium,potassium concentrations with ion-selective electrodes. In this model,NBP at the dose of 80,160 and 240 mg/kg po 15 min after MCAO prevented from brain edema in a dose-dependent manner. A significant reduction of sodium content and an increase in potassium level were observed in all drug-treated groups. It showed that NBP strongly attenuated brain water entry,sodium accumulation and potassium loss. Nimodipine treatment（5mg/kg sc） also reduced brain edema （P<0. 05）. The results suggest that a strong anti-edema activity of NBP may pla4|91|56Updated：2024-04-10
- Abstract：<正> 10 patients with heart disease were received emergent bedside temporary cardiac pacing by the right supraclavicular subclavian vein approach. The indications for the bedside pacing were Ⅲ°atrial-ventricular block in 8 cases and sick sinus syndrome in 2 cases. 7 patients in our group had the Adams-Stokes syndrome for one to more than forty times attacks and another one had frequent ventricular tachycardia or ventricular fibrillation events.4|9|0Updated：2024-04-10
- Abstract：<正> With the aid of methods of polymerase chain reaction / sequence specific primers （PCR/SSP） and polymerase chain reaction-restriction fragment length polymorphism （PCR/RFLP）, the allelic polymorphism of HLA-DR and TNFB loci and susceptibility to systemic lupus erythematosus （SLE） in northern Chinese Han nationality were studied. The genetic analysis of 51 patients with SLE and 106 healthy controls indicated that frequencies of DR2 and DR3 alleles were significantly increased in SLE patients（P< 0.05 and <0. 005, relative risks of 1.77 and 4.01 respectively）, which represent candidate susceptible genes or useful marker for SLE. The frequency of DR5 was found to decrease in SLE patients compared with control population （P<0. 025,relative risk = 0. 38）. It might be an antagonistic or protective allelle or a marker for such allele. Analysis of 51 patients with SLE and 80 healthy control also revealed that the frequency of TNFB’ 2 allele was significantly increased（P<0. 05,RR= 1. 70）. Therefore TNFB’ 2 gene ma4|15|2Updated：2024-04-10
- Abstract：<正> Background. Human herpesvirus-6 （HHV-6） infection is ubiquitous in selected population with sero-prevalence of 60-80%. Little is known for that in China, except few sporadic studies. To understand prevalence of HHV-6 antibody in Chinese population,this seroepidemiological study was conducted.Methods. Sera were collected from 430 healthy persons and donors living in North China,and tested for HHV-6 antibodies using IFA with HHV-6 GS strain passaged on HSB-2 cells as antigen, and titer e-qual or higher than 1:10 was regarded as seropositive.Results. Of the 430 serum samples tested,297 （69.1%） were positive for HHV-6 IgG. Both seropositive rate and GMT in females were significantly higher than those in males,with X2 = 7. 05,P<0. 01 and F = 7.23,P<0. 01,respectively. Statistically significant difference in prevalence of HHV-6 antibody among various age groups was observed in both sexes,with X2=20. 08 and 20. 28,P = 0. 04,respectively,and young children had a higher titer than adults. But, no significant dif4|8|0Updated：2024-04-10
- Abstract：<正> The purpose of this investigation was to offer evidence that immumohistochemical staining is applicable to the determination of morphin distribution in human tissues from cases of opiate associated death. We studied 4 cases of opiate associated death and 2 cases of sudden cardiac death without a history of drug abuse as control. The samples were taken from cerebral cortex and4|5|0Updated：2024-04-10
- Abstract：<正> Aim. To statistic the usage of hypnotics and sedatives in outpatient and emergency clinics prescriptions in one day.Method. Check all prescriptions on April 29, 1987 and November 13, 1995 in order to know the quantity, kind and distribution of hypnotics and sedatives in different outpatient departments.Result. There were 1 319 prescriptions on April 29, 1987. Among them, hypnotics and sedatives were 122（9. 24% ）; in comparing with 2 065 prescriptions on November 13, 1995, hypnotics and sedatives were 141 （6. 82%）. Benzodiazepine used more than 80% in all prescriptions.Conclusion. The possibility of long-term low-dosage using benzodiazepine is present. It must strengthen the control and education to avoid the drug-abuse.4|12|0Updated：2024-04-10
- Abstract：<正> The atrial pacing lead is believed having higher stimulation thresholds and long-term complication rates than ventricular lead, this being one of the factors limiting the use of dual chamber pacing. A prospective study was undertaken to evaluate both atrial and ventricular bipolar lined steroid-eluting leads in long-term dual chamber pacing. There are 81 pairs of leads （Medtronic Capsure SP） used in 81 patients. Bipolar atrial and ventricular stimulation thresholds were measured immediately post implantation and 1,3, 6,12,18 and 24 months. All leads demonstrated low mean stimulation thresholds during the follow-up and more than 94 % of leads could be paced chronically in the atrium and ventricle at 2.5 volts. In conclusion, atrial and ventricular steroid-eluting leads gave excellent stimulation thresholds allowing low energy long-term dual chamber pacing.4|9|1Updated：2024-04-10
- Abstract：<正> In order to describe the magnetic resonance imaging （MRI） findings in hypothalamic-pituitary area and its clinical relevance in patients with idiopathic growth hormone deficiency （IGHD）, the MR imagings of 26 patients with IGHD were analyzed. On MRI, 24 out of 26 cases （92. 3%） showed apparent pituitary upper margin depression; 8 out of 26 cases （30. 8%） showed definite pituitary stalk transection; 22 out of 26 cases （84. 6%） showed absence of the normal posterior pituitary bright spot. The bright lipidlike signal on T1W1 images at the median eminence distal to the breaking point （so-called ectopic posterior lobe） was found in 4 out of 26 cases （15. 4%）. According to the MRI findings of the pituitary stalks, the 26 cases were divided into three groups; group A of 8 cases （31%） characterized by the definite transaction of stalk; group B of 13 cases （50%） defined by the possible stalk transection; and group C of 5 cases （19%） with no definite stalk transection.MRI findings were consistent with the clinica4|15|0Updated：2024-04-10
- Abstract：<正> To assess the presence of HHV-6-Specific DNA in human salivary glands, eighteen specimens of salivary gland tissue were investigated using the polymerase chain reaction. Eight of nine parotid glands, five of seven submandibular glands and one of two sublingual glands were found to have amplification of the HHV-6-specific sequence. The findings suggest that salivary gland tissue is one of the potential sites for HHV-6 persistence following primary infection and that saliva is a vehicle for transmission of the virus.4|13|0Updated：2024-04-10
- Abstract：<正> Three cases of pulmonary leiomyosarcoma were presented. The characteristic clinical features were described with review of literature- In comparison with bronchogenic carcinoma, the leiomyosarcoma has some characteristics: 1) On chest X-ray, it usually appears as a sharply demarcated, even density round mass, growing rapidly within the lung, it unusually accompanies with hilar or mediastinal lymph node metastasis; 2) The preoperative cytological or pathological diagnosis is difficult either by sputum smear or by bronchoscopic biopsy or by fine needle percutaneous aspiration biopsy; 3) Pathological differential diagnosis of leiomyosarcoma of lung from anaplastic lung cancer is difficult. In conclusion, the primary pulmonary leiomyosarcoma is a rare malignant tumor, detecting the present illness seriously, paying attention to the chest X-ray films characterize, early surgical resection is the only way to get diagnosis and effective treatment method.4|9|0Updated：2024-04-10
- Abstract：<正> Human immunodeficiency virus type 1 （HIV-1）is the principal causative agent of AIDS which is threatening human health. The most important antigen of HIV1 is the viral envelope glycoprotein, gp160. The mature form of the HIV-1 gp160 is made up of two major regions:the extracellular （gq120） and the transmembrane （gp41） domains. The gp120 binds with high affinity to CD4. he cellular receptor for HIV-1, and antibodies that prevent the CD4-gq120 interaction are able to block HIV-1 infection. A principal neutralizing deter-4|40|0Updated：2024-04-10
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