Latest Issue
2002 Year 0 Volume 4 Issue
- Abstract：<正>Chief EelitorLiu Depei（刘德培） Associate Chief EdiQiang Boqin（强伯勤） Chen Miaolan（陈妙兰）Peng Ruicong（彭瑞聪）Dai Yuhua（戴玉华）Wu Dechang（吴德昌）H.Baum,P九D.UKChen Miaolan（陈妙兰）Yungchi Cheng,P丸D,USAChu Yonglie（楚雍烈）Dai Yuhua（戴玉华）Dong Yi（董怡）Douglas W.Wilmore,M n,USA4|4|0Updated：2024-04-10
- Abstract：<正>To investigate the roles of mouse erythroid differentiation and denucleation factor （MEDDF）, a novel factor cloned in our laboratory recently, in erylhroid terminal differentiation.Methods. Mouse erythroleukemia （MEL） cells were transfected with eukaryotie expression plasmid pcD-NA-MEDDF. Then we investigated the changes on characteristics of cell growth by analyzing cells growth rate, mitotie index and colony-forming rate in semi-solid medium. The expressions of c-mye and β-globin genes were analysed by semi-quantitative RT-PCR.Results. MEL cells transfected with pcDNA-MEDDF showed significant lower growth rate, mitotic index,and colony-forming rate in semi-solid medium （ P<0. 01）. The percentage of benzidine-positive cells was 32. 8%after transfection. The expression of β-globin in cells transfected with pcDNA-MEDDF was 3. 43 times higherthan that of control （MEL transfected with blank vector, pcDNA3. 1）, and the expression of c-mye decreased by66. 3% .Conclusions. MEDDF can induce differentiation of4|14|0Updated：2024-04-10
- Abstract：<正>To explore the role of insulin-like growth factor 1 （IGF-1） gene expression abnormality in neurotrophic causes of diabetic peripheral neurophathy.Methods. Diabetes was induced in Sprague Dawley rats by alloxan. The parameters were measured as follows: IGF-1 mRNA by reverse transcriptase-polymerase chain reaction （RT-PCR）; IGF-1 peptide by enzyme-linked immunosorbent assay （ELISA）; electrophysiological parameters of nerves by evoked electromyo-gram; morphometric evaluation of sciatic nerves under light microscope and transmission electron microscope.Results. During early diabetic stage, IGF-1 mRNA [ （0. 430 ±0. 031） vs. （0. 370 ±0. 016）, P <0. 01, （0. 430 ±0. 031） vs. （0. 280 ±0. 010） , P <0. 001, respectively], IGF - 1 peptide contents [ （38. 44 ±3. 60） ng/mg vs. （30. 06 ±2. 41） ng/mg, P <0. 01, （38. 44 ± 3. 6） ng/mg vs. （3. 71 ±2. 70） ng/mg, P <0. 001, respectively] in sciatic nerve tissue reduced in diabetic rats with hyperglycemia and varied with severity of diabetic state when compared with n4|29|18Updated：2024-04-10
- Abstract：<正>30 January一2 February,2003—2nd EAACI—Davos Meetingon Basic Immunology in Allergy and Clinical ImmunologyDavos,SwitzedandFor further information: SIAF.0bere Strasse 22.CH。7270 Davos.Switze4|2|0Updated：2024-04-10
- Abstract：<正>To evaluate the effects of alendronate on the structural properties of trabecular bone.Methods. Alendronate was administered at a daily p. o. dose of 0. 5 mg/kg over a 12-week period in hound dogs (n = 8 for both the control and treated group), and the structural indices of the lumbar vertebral (LI and L2) trabecular bone were assessed directly from 3-D images.Results. Treatment with alendronate increased bone volume fraction by 9. 5% and 7. 7% in LI and L2 respectively. Trabecular thickness significantly increased after alendronate treatment, whereas trabecular separation remained constant. The degree of anisotropy for the alendronate - treated group was decreased compared with that of the control group. Bone surface to volume ratio declined significantly in the alendronate-reated group, whereas alendronate induced a higher bone surface density.Conclusion. Alendronate increased the structural properties of canine trabecular bone after short-term treatment at a dose of 0. 5 mg · kg-1·day-1.4|5|0Updated：2024-04-10
- Abstract：<正>To generate phage-displayed anti-idiotypic antibody single chain variable fragments (anti - Id ScFv) to MG7 monoclonal antibody (McAb) directed against gastric carcinoma so as to lay a foundation for developing anti-Id ScFv vaccine of the cancer.Methods. Balb/c mice were immunized i. p. with MG7 McAb conjugated with keyhole limpet hemocyanin (KLH), and mRNA was isolated from the spleens of the immunized mice. Heavy and light chain (VH and VL) genes of antibody were amplified separately and assembled into ScFv genes with a linker DNA by PCR. The ScFv genes were ligated into the phagemid vector pCANTAB5E and the ligated sample was transformed into competent E. coli TGI. The transformants were infected with M13KO7 helper phage to yield recombinant phages displaying ScFv on the tips of M13 phage. After 4 rounds of panning with MG7, the MG7-positive clones were selected by ELISA from the enriched phages. The types of the anti-Id ScFv displayed on the selected phage clones were preliminarily identified by comp4|32|4Updated：2024-04-10
- Abstract：<正>To evaluate the possibility and efficiency of nanoparticle as a new vector in specific gene transference.Methods. Nanoparticle-DNA complex was prepared with Poly- eM-lactic-co-glycolic acid (PLGA) bearing anti-sense monocyte chemotactic protein-1 (A-MCP-1), a specific expression gene, and the package efficiency, release progress in vitro, and the size of the complex were determined. The possibility of the new vector was evaluated with genomic DNA PCR by transferring gene into cultured smooth muscle cells (SMC), cationic lipids as a control. For study in vivo, jugular vein-to-artery bypass grafting procedures were performed on 20 New Zealand white rabbits, of which 6 grafts were transferred with nanoparticle-A-MCP-1 (200 μg), 6 with A - MCP - 1 (200 μ g) by cationic liposome, 4 with LNCX plasmid, and 4 as control. Fourteen days after the grafts were harvested, the expression of A-MCP-1 and its effect on MCP-1 in vein grafts were detected by dot blot, and the morphologic evaluation of grafts was performe4|45|1Updated：2024-04-10
- Abstract：<正>To identify and evaluate mutations in the RPl gene among Chinese patients with retinitis pigmen-tosa (RP).Methods. Leukocyte DNA of 92 RP patients were collected in Hong Kong. Sequence changes of the entire coding region of the RP1 gene were examined using PCR, conformation sensitive gel electrophoresis and DNA sequencing.Results. In total, 1 nonsense mutation and 1 nonsense variant as well as 10 missense alterations were identified in the RPl gene, among which, Arg677Ter was found in 1 RP patient and another nonsense variant, Argl933Ter, was identified in 3 normal individuals and 1 patient with Stargardt’ s disease, suggesting its nonpathogenicity. Arg677Ter is expected to lead to large disruptions of the encoded protein.Conclusions. The nonpathogenicity of Argl933Ter indicates that the C - terminal 224 residues of RPl protein may be not critical for RPl. The most C - terminal truncation previously reported was due to Tyr1053 (1-bp del) and occurred in RP patients. Thus RP can be caused by reduction in4|15|0Updated：2024-04-10
- Abstract：<正>To investigate the relationship of plasma homocysteine (Hcy) level to stroke and genetic factor to elevated plasma Hcy level.Methods. The plasma Hcy level was measured by capillary electrophoresis - ultraviolet detection and the gene polymorphism of N5, N10 - methylenetetrahydrofolate reductase (MTHFR) was studied with PCR-RFLP assay in 43 patients with cortical cerebral infarction and 42 healthy controls.Results. The plasma Hcy level of the patients (19. 3 ± 6. 0 μ mol/L) was markedly higher than that of the controls (13. 7 ± 5. 4μ mol/L) (t = 4. 16, P < 0. 001). There are 3 genotypes, C/C, C/T and T/T, about base - variation of MTHFR gene at locus 677. The plasma Hcy level of the subjects with T/T genotype was higher than that of subjects with other genotypes. However, the frequencies of each genotype and allele were not significantly different between the patients and the controls.Conclusions. The elevated plasma Hcy level is a risk factor for atherothrombotic cerebral infarction, and is related t4|29|7Updated：2024-04-10
- Abstract：<正>Objectives. To compare the effects of losartan, enalapril and their combination in the prevention of left ventricular remodeling (LVRM) after acute myocardial infarction (AMI) in the rat.Methods. AMI model was induced in female SD rats by ligating left coronary artery. Forty-eight hours after the procedure, 83 surviving rats were randomized into one of the following 4 groups : 1) AMI control group (n = 19), 2) losartan group (n =22, 3 mg · kg-1 · d-1), 3) enalapril group (n =20, 1mg · kg-1· d-1), 4) losartan - enalapril combinative group (n = 22, 3 and 1mg · kg-1 · d-1 respectively). 5) sham-operated group (n = 10) and 6) normal rats group (n = 10) were selected randomly to serve as non-infarction controls. Losartan and enalapril were delivered by direct gastric gavage. After 4 weeks of medical therapy, hemodynamic studies were performed in each group, then the rat hearts were fixed with 10% formalin and pathologic analysis on them was performed. Complete experimental data was obtained in 56 rats,4|12|0Updated：2024-04-10
- Abstract：<正>To investigate the high risk factors, clinical features, diagnosis and treatment of pulmonary embolism (PE) during pregnancy and the postpartum period.Methods. Two cases of PE during pregnancy and postpartum period were reported retrospectively.Results. The first case was a pregnant woman with congenital heart disease at 39-week gestation. She underwent a successful cesarean section (CS) because of heart disease, but she manifested tachycardia, tachypnea, cyanosis, and dyspnea suddenly on the 10th day after CS and died soon after the onset of these symptoms. The diagnosis of PE was highly suspected clinically. The second case was a twin-pregnancy woman at 35-week gestation. Because of severe perinatal myocardiopathy, cesarean section was performed. Unfortunately, the patient died abruptly on the operation table. The diagnosis of PE was confirmed by autopsy.Conclusions. The maternal mortality of PE during pregnancy and postpartum period is quite high. Whenever there is any doubt, objective examinations fo4|4|1Updated：2024-04-10
- Abstract：<正>Heat shock protein gp96 is a highly conserved and monomorphic glycoprotein in the endoplasmic reticulum. It functions as molecular chaperone and can associate with a variety of antigenic peptides noncovalently in vivo and in vitro. Recent studies have indicated that gp96 molecules participate in major histocompatibility complex class I - restricted antigen presentation pathway. Immunization of mice with gp96 preparations isolated from cancer cells can elicit a cancer - specific protective T cell immune response that is recallable, which is a prerequisite for gp96 as a therapeutic vaccine against cancers. The immunogenicity of gp96 molecules has been attributed to the antigenic peptides associated with them. These phenomena provide a new pathway for cancer immunothera-py. The mechanism that the gp96 - peptide complex induces specific immune response and the explorations for gp96 - peptide complex as a therapeutic cancer vaccine are reviewed.4|43|3Updated：2024-04-10
- Abstract：<正>To study the biological characteristics of B16-F10 cells transduced with hCD80 gene in vitro, a kind of replication-deficient recombinant adenovirus bearing hCD80 gene (hCD80-rAd) was constructed and several means were utilized to observe the changes of biological characteristics after gene transduced.4|16|0Updated：2024-04-10
- Abstract：<正>Although the cerebral lesions resulting from acute carbon monoxide poisoning are well known, the cardiac function impairment caused by the poisoning still escapes the attention. This article gives more evidence to this point based on clinical study of a series of patients with acute carbon monoxide poisoning. We observed 46 patients and each patient was assessed by physical exami-4|9|0Updated：2024-04-10
- Abstract：<正>To understand the pathophysiologic significances of soluble Fas Ligand (sFasL) and soluble Fas receptor (sFas) in chronic congestive heart failure (CHF) and to determine the relationship of circulating levels of sFasL and sFas to the severity of CHF, the serum sFasL and sFas levels were evaluated in patients with CHF.4|9|1Updated：2024-04-10
- Abstract：<正>The present study analyzed the alteration in [ H + ]i in cultured cerebral cortex neuronal cells of newborn Wistar rats following fluid percussing injury (FPI) and the effect of nimodipine (Nim), D-2-amino-5-phosphonovaleric acid (D-AP-5) and mild hypothermia on neuronal [H + ] i following FPI. Alteration in [H + ]i was measured by means of a confocal laser scanning microscope using BCECF/4|15|0Updated：2024-04-10
- Abstract：<正>AbnormalitiesPregnancy and Its Outcome in Women with MalformedUterusMa Shuiqing,et a1.17(4):242—245Acute myocardial infarctionProcoagulant Effects of Thrombolytic Therapy in AcuteMyocardial InfarctionWang Yanni,et a1.4|4|0Updated：2024-04-10
- Abstract：<正>Chinese Medical Sciences Journal (Chin Med Sci J) is published by the Chinese Academy of Medical Sciences. Chin Med Sci J reports on all aspects of advanced clinical and laboratory medicine in China, while contributions from abroad are welcomed as well. All contributions are subject to peer review. Twenty reprints will be provided free of charge by the publisher after publication.4|4|0Updated：2024-04-10
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