Latest Issue
      2003 Year 2 Issue
      • 桂鸣, Lu-YangWang
        2003(2): 67-74.
        Abstract：<正> Objective. To investigate the properties of voltage-gated sodium （Na+） channels in developing auditory neurons during early postnatal stages in the mammalian central nervous system.Methods. Using the whole-cell voltage-clamp technique, we have studied changes in the electrophysi-ological properties of Na+ channels in the principal neurons of the medial nucleus of the trapezoid body （MNTB）.Results. We found that MNTB neurons already express functional Na+ channels at postnatal day 1 （P1）, and that channel density begins to increase at P5 when the neurons receive synaptic innervation and reach its maximum （3 fold） at P11 when functional hearing onsets. These changes were paralleled by an age-dependent acceleration in both inactivation and recovery from inactivation. In contrast, there was very little alteration in the voltage-dependence of inactivation.Conclusion. These profound changes in the properties of voltage-gated Na+ channels may increase the excitability of MNTB neurons and enhance their phase-l  
          
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      • 2003(2): 74.
        Abstract：<正> 5-8 June, 2003-41st Nordic Lung CongressReykjavik, IcelandFor further information:Congress Secretariat:CongressReykjavik, c/o Lara B.Ptursdttir,Engjateigi 5,105 Reykjavik, Iceland.  
          
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      • 方丕华, NancyLRadtke, TonyW.Simmons, WesleyK.HaistyJr., DavidM.Fitzgerald
        2003(2): 75-79.
        Abstract：<正> Purpose. To evaluate and compare the effects of heart rate on conduction velocity in the cavotricus-pid isthmus （CTI） and septum in patients with and without typical atrial flutter （AF） using electro-anatomic mapping （EAM） of the right atrium （RA）.Methods. Ten patients（age 53+10 yrs,7M/3F）with AF and 13 patients （age 51+11 yrs, 5M/8F） with atrio-ventricular nodal reentrant tachycardia （AVNRT） underwent conventional electrophysionogical study, electro -anatomic mapping and radiofrequency ablation. Using EAMs obtained during coronary sinus pacing at pacing cycle length （PCL） 600 ms, 400 ms, and 300 ms, we evaluated conduction velocities in the CTI and septum of RA in 10 patients with AF and compared EAMs to 13 patients with AVNRT to determine whether the conduction slowing required to maintain AFL was related to changes in volume alone or altered RA electrophysiology.Results. Conduction velocities in CTI and septum were significantly slower at all PCL when AF was compared to AVNRT（P<0.05）. Additionally, i  
          
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        Updated：2024-04-10
      • 郑华川, 李晓晗, 孙晋民, 曹乾, 辛彦, 张荫昌
        2003(2): 80-86.
        Abstract：<正> Objective. To investigate the role of matrix metalloproteinase-7 （MMP-7） expression in caricinogenesis and progression of gastric cancer.Methods. We studied MMP-7 expression and microvessel density （MVD） in adjacent mucosa and primary foci of 113 cases of gastric cancer by streptavidin -biotin -immunoperoxidase method using anti -MMP-7 and anti-CD34 antibodies. MMP-7 expression and mean MVD were compared with clinicopatholog-ical features of gastric cancer, with the relationship between MMP-7 expression and MVD concerned in gastric cancer.Results. MMP-7 showed positive expression in adjacent mucosa of gastric cancer （29.20%, 33/113）, less than that in gastric cancer （69.03%, 78/113）. MMP-7 expression in primary foci of gastric cancer was positively correlated with tumor size, invasive depth, metastasis and TNM staging （P<0.05）, but not with differentiation or growth pattern of gastric cancer （P>0.05）. Positive correlation of mean MVD with tumor size, invasive depth, metastasis and TNM staging was found  
          
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        Updated：2024-04-10
      • 宋毅, 黄光琦, 贺国丽
        2003(2): 87-92.
        Abstract：<正> Objective. To understanding the molecular mechanisms in invasion and metastasis of the ovarian carcinoma, we investigate a novel candidate metastasis-associated gene （ MTA1） and nm23Hl mRNA expression and mutation in ovarian carcinoma.Methods. Twenty primary ovarian carcinoma specimens, 20 corresponding lymph nodes and 8 normal ovarian was examined for mRNA expression and mutation of MTA1 and nm23Hl genes by reverse-transcription ploymerase chain reaction （RT-PCR） and RT-PCR-SSCP analysis. The level of the expression was determined by the relative optic desity （ROD） of the PCR products.Results. The frequency of MAT1 overexpression was 100% （7/7） in primary ovarian carcinoma with metastasis but only 38.5% （5/13） in those without metastasis （P=0.0103）. Overexpression of MAT1 was observed in 87.5%（6/7） of lymph nodes with metastasis but only 23%（3/13） of lymph nodes without metastasis （P=0.0118）. In contrast with MAT1, low expression of nm23Hl mRNA was seen in 7 of 7 o-varian carcinoma with metastasis but  
          
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        Updated：2024-04-10
      • 于永强, 董卫东, 李欣, 李树华, 周小榕
        2003(2): 92.
        Abstract：<正> The present study analyzed the DNA content（ratio of heterploid,RH）,s-phase cell（SPC）per-centage,the proliferating cell nuclear antigen（PC-NA） labelling index and area of nucleolar organizerregion （AgNOR） in 57 patients with laryngeal can-cer. The results were shown in Table 1.  
          
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      • 丁晓曼, 冷金花, 郎景和, 李华军
        2003(2): 93-96.
        Abstract：<正> Objective. To evaluate the surgery in simple ovarian cysts.Methods. From Jan.1994 to Dec.1999, 221 women with simple ovarian cysts were admitted into our hospital. The diameter of cysts was <5 cm in 76 cases, and was>5 cm in 145 cases ultrasonically. One hundred and eighty-four patients underwent laparosocopy, and thirty-seven underwent laparotomy.Results. Histological findings showed no malignancy in this series. Simple cysts, paraovarian cysts and corpus luteum were found histologically in 90.8% of cases with cysts <5 cm, and 60% of those with cysts >1 cm respectively, their difference was significant （x2=37.13, P<0.001）. The simple cysts, paraovarian cysts and corpus luteum cysts were found in 81.5% of postmenopausal women and 84.0% of pre-menopausal women.Conclusion. Patients with cysts >1 cm are indicated for surgical procedures, while a period of fol-lowup is acceptable for patients with cysts <7 cm, and surgery is advisable if the cyst is persistent during followup. Postmenopausal women with cyst  
          
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      • 秦明伟, 余卫, 徐苓, 田均平, 邢小平, 孟迅吾
        2003(2): 97-99.
        Abstract：<正> Objective. To study the whole body bone mineral changes in normal subjects.Methods. Bone mineral was measured by dual X-ray absorptiometry （DXA） in 292 normal subjects aged 1079 years, including 152 females and 140 males. They were divided into age-groups by decade.Results. In males, the ages with peak value of total bone mineral content （TBMC）, total bone mineral density （TBMD）, anteroposterior spine BMD（L2-4 BMD） were in the 30-39,20-39, 20-29 age-groups respectively. In females, they were all in the 30-39 age-group. Peak values of TBMC, TBMD, L2-4 BMD were higher in males than those in females. Loss of bone mineral for females was more pronounced than that for males. Loss of L2-4 BMD was more pronounced than TBMD.Conclusion. This study provides the normal bone mineral data of whole body for males and females, and the characteristic of changes.  
          
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        Updated：2024-04-10
      • 罗新锦, 许建屏, 沈向东, 陈霞
        2003(2): 100-104.
        Abstract：<正> Objective. To investigate the effect of peritoneal dialysis on fluid balance and outcome in children who receive cardiac operation.Methods. From July to Dec. 2000, 12（2.08%） patients of 576 consecutive children who underwent heart operation required peritoneal dialysis because of acute renal failure. The mean age of these 12 patients was （2.9-2.0） years （range, 5 months-7 years） and the mean body weight was （12+3） kg （range, 7.4-18.5 kg ）.Results. The interval between the operation and the initiation of peritoneal dialysis was （21.2+11.4） hours （4.4-42 hours）. The duration of peritoneal dialysis was （6.3+4.8） days （0.47-15 days）. Mortality in these 12 patients was 25%. Fluid removed by peritoneal dialysis was（34.7+17.8） ml . kg-1 . day-1. Asymop-tomatic hypokalemia, thrombocytopenia and hyperglycemia were frequent complications, which were easily managed. Hemodynamics and pulmonary function improved during the study period.Conclusion. The early initiation of peritoneal dialysis is an effective and safe  
          
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        Updated：2024-04-10
      • 崔玉尚, 张志庸, 徐协群
        2003(2): 105-110.
        Abstract：<正> Objective. To investigate the changes and influencing factors of early postoperative pulmonary function of thoracotomy.Methods. Pre-and early postoperative pulmonary function was studied in 64 consecutive cases with optimal thoracotomy. Pain assessment was done before pulmonary function test, and the chief complaints of patients were recorded after the procedure. The changing curves of pulmonary function were done and the differences associated with groups, surgical styles, pain assessment, epidural analgesia, chief complaint and preoperative conditions were analyzed.Results. Pulmonary function was severely lowered to about 40% of the base line on the first day, and it was rehabilitated to about 60% of the base line on the eighth day. There was a greater gradient on the recovery curve on the 3rd and 4th days. Epidural analgesia was able to improve pain relaxation and pulmonary function in some degree. Single-factor analysis showed that postoperative pain, postoperative day and surgical style were the si  
          
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      • 张小明, 汪忠镐
        2003(2): 111-115.
        Abstract：<正> Objective. Report the results of interventional or semi-interventional techniques for 173 patients with Budd-Chiari syndrome.Method. This group included 120 males and 53 females. The pathologic lesions composed of localized complete occlusion of inferior vena cava （IVC） （78）, IVC stenosis （49）, IVC membrane with a hole （37）, membrane of hepatic vein （HV） （3）, IVC thrombosis （4）, IVC membrane with thrombosis （2） and IVC lesion with occlusion of HV （32）. Treatment methods included that I: Percutaneous transinferior vena cava angioplasty （PTA） （76）; II: IVC PTA with stent （59）; III: Percutaneous transhepatic vein recanalization （3）; IV: IVC thrombolysis through a catheter （4）; V; Combined transcardiac and trans-femoral venous membranotomy and balloon dilation （22）; VI: V and stent （17）; VII; Stenting during radical surgery （3）; VIII: Additional operation after intervention （23）.Results. The immediate technique success rate for intervention was 90.1%, for the semi-intervention was 100%. The IVC pressure was  
          
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      • 管珩, 郑月宏, 李拥军, 刘昌伟, 刘暴, 叶炜
        2003(2): 116-119.
        Abstract：<正> Objective. To describe our surgical experience on rupture of abdominal aortic aneurysm .Methods. Two cases of ruptured aortic aneurysms with severe complication were analyzed. Aorta reconstruction procedures were performed using bifurcated e-PTFE grafts during emergency operation. Diagnosis, preoperadve resuscitation, emergency surgical intervention, and postoperative complications of these patients were summarized and discussed.Results. Rupture of aortic aneurysm in both patients presented as a huge retroperitoneum haematoma by computed tomography scan. They were successfully saved by prompt body fluid compensation, emergency procedure, intraoperative resuscitation, and postoperative intensive care.Conclusions. Correct diagnosis, prompt surgical management, immediate intraoperative proximal aorta clamping during procedure, and effective management of postoperative complications were the key points to successful treatment of ruptured aortic aneurysm.  
          
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      • 陶蔚, 任祖渊, 苏长保, 王任直, 杨义, 马文斌
        2003(2): 120-123.
        Abstract：<正> Objective. To clarify the frequency, presentation, associated factors, treatment and outcome of hy-ponatremia after transsphenoidal surgery of pituitary adenomas.Methods. Retrospectively reviewed the database of 183 patients who underwent transsphenoidal surgery of pituitary adenomas between January 1999 and June 2000 in our department.Results. 38.8% （71/183） had postoperative hyponatremia. Among them, 59.2% （42/71） appeared on the 4th to 7th day postoperatively. 59.2% （42/71） presented with nausea, vomiting, headache, dizziness, confusion and weakness. Hyponatremia was related to age, tumor size and adenoma type, but not related to sex and degree of resection. Treatment consisted of salt replacement and mild fluid restriction in 4 patients and salt and fluid replacement in 67 patients. Hyponatremia resolved within 16 days in all the patients.Conclusions. Hyponatremia often appeared about 7 days after transsphenoidal surgery of pituitary adenomas, especially in elderly and patients with macroadenomas an  
          
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      • 应作霖, 孙建方, 刘珊
        2003(2): 124-127.
        Abstract：<正> Objectives. To detect telomerase activity in patients with mycosis fungoides （MF） and to study the role of telomerase in the tumorigenesis of MF.Methods. The technique of PCR-ELISA was employed to detect telomerase activity in 35 patients with various stages of MF.Results. 92.3% tumor stage of MF, 78.6% plaque stage of MF and 75.0% patch stage of MF had positive telomerase activity. The control samples had no telomerase activity. Telomerase activity in tumor stage of MF was significantly higher than that in plaque stage, while the latter was higher than that in patch stage. Telomerase activity was correlated with the stage of MF.Conclusion. High level of telomerase activity frequently occurred in patients with MF, suggesting that telomerase might play an important role in the tumorigenesis of MF and is a useful marker for the diagnosis of MF possibly.  
          
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      • 袁明秀, 蒋晖, 邓爱萍, 周晴中
        2003(2): 128-131.
        Abstract：<正> Objective. To study the characteristics of changes of LDH enzyme patterns of mice under slight hypoxia.Methods. Mice treated with artificial hypoxia, various tissues were made for the test of LDH enzymatic activity by the specific staining technique. LDH （1 -5） relative percentage enzymatic activity （RPEA） were measured with CS-910 dual-wavelength thin layer chromatography scanner.Results. The RPEA of LDH isozymes of various tissues after slight hypoxia shifted to the isozymes LDH1 and LDH2, whose principal subunits are H subunits, and the RPEA of LDH,（H4）, LDH2（H3M） increased, while RPEA of LDH5（M4） in various tissues decreased prominently except the cardiac muscle, and that of LDH4（HM3） decreased as well. After polyacrylamide gel electrophoresis （PAGE） of the hypoxia treated cardiac muscle specimen was made, activity subbands originated regularly in the isozyme patterns of LDH, with the regularity of LDH1 （0 subband）, LDH2 （0-1 subbands）, LDH3 （0-2 subbands）, LDH4 （1-3 subbands）, LDH5 （2-4 subbands）.  
          
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      • 李鹏宇, 孙靖中, 张小桥
        2003(2): 132.
        Abstract：<正> In this study,we investigated preoperatlve diag-nostic value of telomerase activity for breast cancerand the correlation between biological characteristicsof breast tumor and telomerase activity.  
          
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