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2011 Year 26 Volume 2 Issue
- Abstract：Objective To investigate the possible involvement of erythr opoietin (EPO)/erythropoietin receptor (EPOR) system in neovascularization and vascular regeneration in diabetic retinopathy (DR). Methods EPOR positive circulating progenitor cells (CPCs: CD34+) and endothelial progenitor cells (EPCs: CD34+KDR+) were assessed by flow cytometry in type 2 diabetic patients with different stages of DR. The cohort consisted of age- and sex-matched control patients with out diabetes ( n=7), non-proliferative DR (NPDR, n=7),non-proliferative DR (PDR, n=8), and PDR complicated with diabetic nephr opathy (PDR-DN, n=7).Results The numbers of EPOR+ CPCs and EPOR+ EPCs were reduced remarkably in NPDR compared with the control group (both P＜0.01), whereas rebounded in PDR and PDR-DN groups in varyingdegrees. Similar changes were observed in respect of the proportion of EPOR+CPCs in CPCs (NPDR vs. control, P＜0.01) and that of EPOR+ EPCs in EPCs (NPDR vs. control, P＜0.05).Conclusion Exogenous EPO, mediated via the EPO/EPOR system of EPCs, may alleviate the impaired vascular regeneration in NPDR, whereas it might aggravate retinal neovascularization in PDR due to a rebound of EPOR+EPCs associated with ischemia.Keywords：circulating progenitor cells;endothelial progenitor cells;erythropoietin re-ceptor;diabetic retinopathy4|1723|2Updated：2024-04-10
- Abstract：Objective To study the regulatory rolesof SIRT1 on EZH2 expression and the further effects on EZH 2’ s repression of target gene expression.Methods The stable SIRT1 RNAi and Control RNAi HeLa cells were established by infection with retroviruses expressing shSIRT1 and shLuc respectively followed by puromycin selection. EZH2 protein level was detected by Western blot in either whole cell lysate or the fractional cell extract. Reverse transcription-polymerase chain reaction was performed to detect the mRNA level of EZH2. Cycloheximide was used to treat SIRT1 RNAi and Control RNAi cells for protein stability assay. Chromatin immunoprecipitation(ChIP) assay was applied to measure enrichment of SIRT1, EZH2, and trimethylated H3K27 (H3K27me3) at SATB1 promoter in SIRT1 RNAi and Control RNAi cells.Results Western blot results showed that EZH2 protein level increased upon SIRT1 depletion. Fractional extraction results showed unchanged cytoplasmic fraction and increased chromatin fraction of EZH2 protein in SIRT1 RNAi cells. The mRNA level of EZH2 was not affected by knockdown of SIRT1. SIRT1 recruitment was not detected at the promoter regionof EZH2 gene locus. The protein stability assay showed that the protein stability of EZH2 increases upon SIRT1 knockdown. Upon SIRT1 depletion, EZH2 and H3K27me3 recruitment at SATB1 promoter increases and the mRNA level of SATB1 decreases. Conclusions Depletion of SIRT1 increases the protein stability of EZH2. The regulation of EZH2 protein level by SIRT1 affects the repressive effects of EZH2 on the target gene expression.Keywords：SIRT1;EZH2;Polycomb repression complex;trimethylated H3K274|3173|1Updated：2024-04-10
- Abstract：Objective To determine if multi-detector CT (MDCT) characterization of plaque is correlatedwith the classification of acute coronary syndrome (ACS). Methods Altogether 1900patients were examined by MDCT from December 2007 to May 2009, of whom 95patients fulfilled the criteria of ACS. Those patients were divided into thediscrete plaque group ( n=61) anddiffuse plaque group ( n=34) based onthe findings in MDCT. The clinical diagnosis of ACS and CT results wereanalyzed, including segment stenosis score, segment involvement score, 3-vesselplaque score, left main score, calcification score, and remodeling index. Theincidences of major adverse cardiac events in follow-up period were alsorecorded.ResultsThe patients of the diffuse plaque groupwere older than those of the discrete plaque group ( P＜0.0001). The diffuse plaque grouppresented more cases of hypertension, peripheral artery disease, diabetes, and heart failure than discrete plaque group (all P<0.05). All the 5 patients with ST-segment elevation myocardialinfarction were found in discrete plaque group. The segment stenosis score ofthe discrete plaque group was lower than that of the diffuse plaque group(5.15±3.55 vs. 14.91±5.37, P＜0.001). The other four scores demonstrated significant inter-group difference as well (all P<0.05). The remodeling index of thediscrete plaque group was higher (1.12±0.16 vs.0.97±0.20, P＜0.05). Follow-up data showed that major adverse cardiac eventsoccurred more frequently in diffuse plaque group than in discrete group (29.41% vs. 11.48%, P=0.0288).Conclusions Characteristics of discrete and diffuseplaques may be significantly different among different classes of ACS. Thediffuse plaque may present higher risk, correlated to higher mortality. Thediagnosis of discrete and diffuse plaques by MDCT would provide a new insightinto the prognosis and treatment of ACS.Keywords：acute coronary syndrome;coronary plaque;multi-detector CT4|1913|1Updated：2024-04-10
- Abstract：Objective To explore the effect of α- galactosyleramide( α-GalCer ) on immune reconstitution under acute graft-versus-host disease (aGVHD). Methods BALB/c mice were transplanted wit hallogeneic C57BL/6 bone marrow cells and splenocytes (both 1×107)after receiving lethal total-body irradiation. α-GalCer (100 ug/kg) or vehicle (dimethylsulfoxide) was administered intraperitoneally immediately after transplantation. The effects of α-GalCer on immune reconstitution,proliferation of T cells and B cells, hematopoiesis,and thymic microenvironment were assessed. Results The α-GalCer group exhibited higher percentages of CD3+,CD4+, CD8+, B220+, CD40+, and CD86+cells compared with the vehicle group . The number of colony forming unit per 1000 CD34+ cells in the α-GalCer group was higher than in the vehicle group ( P=0.0012).In vitro proliferation assays showed that the α-GalCer group had higher percentages of CD3+, CD4+, CD8+,and B220+ cells compared with the vehicle group. As for the results of in vivo proliferation assays, the numbers of CD3+, CD4+, CD8+, and B220+cells were higher in the α-GalCer group than in the normal group ,especially the number of B220+ cells ( P=0.007).Significant difference was not found in thymocyte count between the α-GalCer group and the vehicle group, nor in the percentages of CD3+, CD4+, and CD8+ cells.Conclusion Administration of α-GalCer after allogeneic bone marrow transplantation may promote immune reconstitution in the presence of aGVHD.Keywords：immune reconstitution;α-galactosyleramide;bone marrow transplantation4|1815|1Updated：2024-04-10
- Abstract：Objective To investigate the independent risk factors of traumatic brain injury (TBI) prognosis. Methods A retrospective analysis was performed in 885 hospitalized TBI patients from January 1, 2003 to January 1,2010 in the First Affiliated Hospital of Medical College of Xi’an Jiaotong University .Single-factor and logisticregression analysis were conducted to evaluate the association of different variables with TBI outcome. Results The single-factor analysis revealed significant association between several variables and TBI outcome, including age ( P=0.044 for the age group 40-60, P＜0.001 for the age group ≥60), complications ( P<0.001), cerebrospinal fluid leakage( P<0.001), Glasgow Coma Scale(GCS) ( P<0.001), pupillary light reflex ( P<0.001), shock ( P<0.001), associated extra-craniallesions ( P=0.01), subdural hematoma ( P<0.001), cerebral contusion ( P<0.001), diffuse axonal injury ( P<0.001), and subarachnoid hemorrhage( P<0.001), suggesting theinfluence of those factors on the prognosis of TBI. Furthermore, logistic regression analysis identified age, GCS score, pupillary light reflex, subdural hematoma,and subarachnoid hemorrhage as independent risk factors of TBI prognosis. Conclusion Age, GCS score, papillary lightreflex, subdural hematoma, and subarachnoid hemorrhage may be risk factors influencing the prognosis of TBI. Paying attention to those factors might improve the outcome of TBI in clinical treatment.Keywords：traumatic brain injury;prognosis;risk factors4|2115|2Updated：2024-04-10
- Abstract：Objective To observe the imaging findings of congenital megaureter in order to enhance the understanding of this disease. Methods Image data of 5 patients with congenital megaureter and 2 misdiagnosed patients were analyzed, and image findings of congenital megaureter were summarized.Elscint Prestig 2.0T superconductive magnetic resonance urography (MRU) with conventional sequence (spin-echo, T1WI560 ms/16 ms; fast spin-echo, T2WI 9600 ms/96 ms ) was performed.Raw data were acquired with fastspin-echo sequence from heavy T2-weighted image (9600 ms/120 ms). Post-processing method of MRU was the maximum intensity projection with three-dimensional reconstruction in the workstation. Intravenous pyelography (IVP) was conducted, in which X-rayfilms were taken 7 minutes, 15 minutes, and 30 minutes after injecting contrast agent, exceptthat in 2 patients the films were taken delayed at 60 and 90 minutes .X-ray retrograde pyelography was performed on 2 patients, successful in one butfailed in the other.Results The dilated ureter showed hypointensity on T1-weighted images and hyperintensity on T2-weighted images in conventional MRI. The mass wall was intact, uniform in thickness, and showing hypointensity on T1-weighted and T2-weighted images. The MRU images showed a retroperitoneal mass appearing as an elongated tubular cystic structure spreading from kidney to bladder. MRU also revealed dilated calices and renal pelvis, pelviureteric obstruction, and renal duplication. The main signs of congenital megaureter in X-urography was significant dilatation of ureter, or normal renal pelvis with ureter dilatation, hydronephrosis, deformity, and displacement. Conclusions MRU with X-urography could visualizethe characteristics of congenital megaureter, including the dilation of renal pelvis and ureter, calculi, urinary tract duplication, and stenosis location. The two techniques can complement each other in disease diagnosis and provide more detailed information for preoperative treatment.Keywords：congenital megaureter;magnetic resonance urography;intravenous pyelography4|1856|0Updated：2024-04-10
- Abstract：Objective To detect the change of nerve growth factor (NGF) level in human amniotic fluid during gestation, and to explore the relationship between this change and fetal ventriculomegaly (VM). Methods The studied subjects (collected from 2004 to 2007) were divided into four groups, including the second-trimester pregnancy group (n=113), third-trimester pregnancy group (n=110), fetal cerebral VM group (n=12), and healthy control group (n=12) which matched with the VM group in gestational weeks. The amniotic fluid specimens were obtained during amniocentesis or cesarean section. The NGF levels in amniotic fluid were detected with enzyme-linked immunosorbent assay. Results A significantly negative correlation was found between gestational age and the NGF level in amniotic fluid (r=−0.6149, P<0.0001). The NGF level in patients with fetal VM was significantly lower than that in healthy controls (33.95±29.24 pg/mL vs. 64.73±16.21 pg/mL, P=0.024). Conclusion NGF levels in amniotic fluid may be a sensitive marker for fetal VM.Keywords：nerve growth factor;amniotic fluid;fetal ventriculomegaly4|1629|0Updated：2024-04-10
- Keywords：hepatocellular carcinoma;radiotracer;positron emission tomography;biological behavior4|1735|0Updated：2024-04-10
- Keywords：C-peptide;diabetes;encephalopathy4|1838|2Updated：2024-04-10
- Keywords：breast tumor;fibromatosis;hydrophilic polyacrylamide gel;breast augmen-tation4|2194|0Updated：2024-04-10
- Keywords：endodontics;maxillary first molar;root canal system4|2111|0Updated：2024-04-10
- Keywords：penile fracture;surgical intervention4|1809|0Updated：2024-04-10
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