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2011 Year 26 Volume 3 Issue
- Abstract：Objective To evaluate the predictive factors of refractory glaucoma treated with diode laser transscleral cyclophotocoagulation (TSCP) in a midterm observation. Methods Fifty-four consecutive patients (54 eyes) with refractory glaucoma were enrolled and underwent TSCP (power of 1.5-2.0 W, exposure time of 2 seconds and 20-50 applications). Patients were regularly followed up for 12 months. Intraocular pressure (IOP) was measured before TSCP treatment and at each follow-up visit. Patients were divided into the sensitive and insensitive groups according to the effect of a single treatment of TSCP on IOP. The success rate was defined as the percentage of eyes achieving an IOP between 5 and 21 mm Hg following TSCP therapy at their final follow up visit. Difference of age between the sensitive and insensitive groups was evaluated with Independent-samples T test and that of gender distribution of two groups was evaluated with Fisher’s exact test. The success rate of different types of glaucoma to TSCP was calculated. Results A total of 32 patients were sensitive to TSCP (sensitive group) and 22 patients were insensitive to TSCP (insensitive group). The success rates of female (77.8%, 14/18) and cases being over 50 years (71.4%, 20/28) were higher than those of male (50.0%, 18/36) and ones being under 50 years (46.2%, 12/26) (all P>0.05). The age of the sensitive and insensitive groups was 56.0±15.5 and 46.4±15.4 years, respectively (P=0.029). The age (P=0.029) and gender distribution (P=0.046) of the two groups had significant difference. The success rate of neovascular glaucoma, primary angle-closure glaucoma, primary open angle glaucoma, traumatic glaucoma, uveitic glaucoma, and secondary glaucoma after silicone oil injec-tion to TSCP was 57.1%, 100.0%, 50.0%, 20.0%, 81.8%, and 0%, respectively. Conclusion Age, gender, and type of glaucoma appear to be associated with effect of TSCP on IOP control.Keywords：predictive factor;diode laser;transscleral cyclophotocoagulation;intraocular pressure4|2748|0Updated：2024-04-10
- Abstract：Objective To assess the time course of Q value after myopic laser-assisted in situ keratomileusis (LASIK) and preliminarily evaluate the determinants of the difference of Q value between before and after LASIK. Methods We performed a retrospective, longitudinal investigation on patients undergoing wavefront optimized LASIK therapy for emmetropization. A total of 418 eyes from 222 cases were examined preoperatively, and partly followed up at one week (172 eyes), one month (134 eyes) and three months (51 eyes) after surgery. The horizontal, vertical and total Q values of cornea were calculated from eccentricity measured at the central 6-mm corneal zones respectively. Potential determinants of the change of Q value were analyzed using multiple linear regressions. Results The mean Q value was -0.17±0.13 preoperatively, and 0.99±0.70, 0.97±0.66, and 0.86±0.41 one week, one and three months postoperatively, respectively. One way analysis of variance (ANOVA) demonstrated significant differences between measurements made before surgery and at all postoperative times (at one week, one and three months; all P<0.0001, Bonferroni post hoc), but no significant differences were found among postoperative groups. Significant differences of Q values between horizontal and vertical meridians were found before surgery and at all postoperative times (all P<0.0001). Multiple regression analysis revealed that change of Q value significantly correlated with manifest refraction spherical equivalent (r=0.116, P<0.0001) and axial length (r=0.264, P<0.0001). Conclusions Over the study period, the primary changes in Q value occur within 1 week after surgery, and then become slightly decreased and nearly stable. Manifest refraction spherical equivalent and axial length play a significant role in the change of postoperative Q value.Keywords：corneal asphericity;Q value;myopia;laser-assisted in situ keratomileusis6|2051|0Updated：2024-04-10
- Abstract：Objective To investigate the effect of multiple coatings of the one-step self-etching adhesive on immediate microtensile bond strength to primary dentin. Methods Twelve caries-free human primary molars were randomly divided into 2 groups with 6 teeth each. In group 1, each tooth was hemisected into two halves. One half was assigned to control subgroup 1, which was bonded with a single-step self-etching adhesive according to the manufacturer’s instructions; the other half was assigned to experimental subgroup 1 in which the adhesive was applied three times before light curing . In group 2, the teeth were also hemisected into two halves. One half was assigned to control subgroup 2, which was bonded with the single-step self-etching adhesive according to the manufacturer’s instructions; the other half was assigned to experimental subgroup 2 in which three layers of adhesive were applied with light curing each successive layer. Microtensile bond strength was immediately tested after specimen preparation. Results When the adhesive was applied three times before light curing, the bond strength of the experimental subgroup 1 (n=33, 57.49±11.61 MPa) was higher than that of the control subgroup 1 (n=31, 49.71±11.43 MPa, P<0.05). When using the technique of applying multiple layers of adhesive with light curing each successive layer, no difference of immediate bond strength was observed between the control subgroup 2 and the experimental subgroup 2 (P>0.05). Conclusion Multiple coatings of one-step self-etching adhesive can increase the immediate bond strength to primary dentin when using the technique of light-curing after applying three layers of adhesive.Keywords：self-etching adhesive;microtensile bond test;primary dentin;multiple coatings4|2797|1Updated：2024-04-10
- Abstract：Objective To assess the prevalence of anti-alpha-enolase antibody in systemic autoimmune diseases in Chinese patients and its role in endothelial cell apoptosis. Methods The reactivity of anti-alpha-enolase antibody in a variety of autoimmune disorders in Chinese patients was evaluated by dot blot assay. Endothelial cell apoptosis was investigated by in vitro incubation of endothelial cells with IgG purified from anti-alpha-enolase antibody-positive sera, with or without pre-incubation with recombinant alpha-enolase. Results Anti-alpha-enolase antibody was prevalent in different systemic autoimmune diseases with relatively high reactivity in Chinese patients. In vitro incubation of endothelial cells with IgG containing anti-alpha-enolase antibody induced apoptosis in a time- and dose-dependent manner. Apoptosis was partly inhibited by pre-incubation of the endothelial cells with recombinant alpha-enolase. Conclusions Our data suggest that alpha-enolase is a common auto-antigen recognized by anti- endothelial cell antibodies in connective tissue disease. Interaction between alpha-enolase and its autoantibody plays a role in endothelial cell apoptosis. Changes other than cell killing may contribute to the pathogenesis of endothelial damage and microvascular lesions.Keywords：anti-alpha-enolase antibody;apoptosis;endothelial cell4|2819|3Updated：2024-04-10
- Abstract：Objective To investigate the expression levels of serum hypoxia inducible factor 1 alpha (HIF-1α) and vascular endothelial growth factor (VEGF) pre- and post-transcatheter arterial chemoembolization (TACE) in patients with primary liver cancer (PLC), and correlations between prognosis factors and serum HIF-1α as well as VEGF levels. Methods Forty consecutive patients fulfilling diagnostic criteria for PLC undergoing TACE from March 2008 to May 2009 were enrolled into the study. The serum HIF-1α and VEGF levels of PLC patients pre- and 1 day, 1 week, 1 month post-TACE were analyzed using ELISA, and compared with that of 20 healthy volunteers. Patients were divided into complete response (CR) and partial response (PR), stable disease (SD), progressive disease (PD) groups according to the therapeutic efficacy. Pearson correlation was used to analyze the correlation between different clinical variables and serum HIF-1α and VEGF levels before TACE, and correlation between serum HIF-1α and VEGF levels was also evaluated. Results The expression levels of serum HIF-1α and VEGF in PLC patients were 154.94±83.29 and 264.00±148.10 pg/mL pre-TACE, and both of them were significantly higher than those in control group (23.84±8.15 and 69.78±21.42 pg/mL, all P<0.01). One day after TACE, both serum HIF-1α (570.64± 230.87 pg/mL) and VEGF levels (362.07±102.25 pg/mL) reached the peak values (all P<0.01). One week post-TACE, expression levels of them were decreased (198.62±92.11 and 283.52±145.46 pg/mL respectively), but still significantly higher than those before TACE (all P<0.01). The levels of both HIF-1α (133.96±57.02 vs. 255.74±123.44 pg/mL) and VEGF (150.96±84.89 vs. 368.95±161.90 pg/mL) in CR group 1 month post-TACE were significantly lower than those in PR+SD+PD group (all P<0.01). The level of serum HIF-1α was positively correlated with serum VEGF level (r=0.42, P<0.001). Both serum HIF-1α and VEGF levels were observed to be correlated with portal vein tumor thrombi (P<0.05) and metastasis (P<0.05). Conclusion The HIF-1α and VEGF might play an important role in relapse of PLC. They might be considered as predictors of the efficacy of TACE and metastasis of PLC.Keywords：liver neoplasms;transcatheter arterial chemoembolization;hypoxia inducible factor 1 alpha;vascular endothelial growth factor4|3376|5Updated：2024-04-10
- Abstract：Objective To analyze the relationship between karyotypes and clinic features of patients with primary amenorrhea. Methods G banding was done for 340 patients with primary amenorrhea to facilitate indivi- dual chromosome identification, and if specific staining for certain portions of the chromosome was nec- essary, C banding was used. The clinical data were recorded by physical examination and ultrasound scanning. Results Karyotype analysis of the 340 patients revealed that 180 (52.94%) patients had normal female karyotypes and 160 (47.06%) patients had abnormal karyotypes. The abnormal karyotypes included abnormal X chromosome (150 patients), mosaic X-Y chromosome (4 patients), abnormal autosome (5 patients), and X-autosome translocation (1 patient). The main clinical manifestations in patients with primary amenorrhea were primordial or absent uterus (95.9%), invisible secondary sex features (68.8%), little or absent ovary (62.6%), and short stature (30.0%). The incidence of short stature in patients with X chromosome aberration (46%, 69/150) was significangly higher that in patients with 46, XX (9.44%, 17/180) as well as 46, XY (6.67%, 3/45; χ2=146.25, P=0.000). All primary amenorrhea patients with deletion or break-point at Xp11.1-11.4 were short statures. Conclusions One of the main reasons of primary amenorrhea is choromosome abnormality, especially heterosome abnormality. It implies the need to routinely screen chromosomal anomalies for such patients. There might be relationship between Xp11.1-11.4 integrity and height improvement.Keywords：primary amenorrhea;Turner Syndrome;karyotype4|4164|1Updated：2024-04-10
- Abstract：Objective To investigate the clinical features, diagnosis methods, therapeutic principles of intestinal Behcet’s disease. Methods The clinical data of 45 patients with confirmed intestinal Behcet’s disease admitted to Peking Union Medical College Hospital from August 1998 to April 2010 were retrospectively analyzed. Results The clinical courses of patients with intestinal Behcet’s disease were from 26 days to 33 years, and the average duration was 6.32±1.01 years. The appearance of extra-gastrointestinal symptoms was significantly earlier than that of gastrointestinal symptoms (7.35±1.39 years vs. 3.24±0.82 years, P<0.05). The predominant gastrointestinal manifestations were right lower quadrant pain (95.56%) and hematochezia or melena (40.00%). Misdiagnosis occurred in 17 cases. In patients without systemic medicine therapy before surgery, the incidence of postoperative infection of incision site and abdominal cavity was significantly higher than that in those undergoing systemic medicine therapy (80.00% vs. 0%, P<0.05). Conclusions Because of the diversity of gastrointestinal manifestations, intestinal Behcet’s disease is easily misdiagnosed. The systemic medical therapy before surgery could decrease the incidence of infection of incision and abdominal cavity.Keywords：Behcet’s disease;gastrointestinal tract4|2399|0Updated：2024-04-10
- Abstract：Objective To investigate the clinical characteristics of primary adult renal sarcoma. Methods A total of 1654 cases with adult renal tumors were treated during 1985 to 2009 in Peking Union Medical College Hospital. Of all, 17 cases were diagnosed as primary renal sarcoma and underwent radical nephrectomy. The clinical features of 17 such patients were retrospectively analyzed. Results The first symptom of 10 (59%) cases in all renal sarcomas was abdominal mass. The pathological diagnosis was leiomyosarcoma (7 cases), rhabdomyosarcoma (2 cases), malignant fibrous histiocytoma (2 cases), low-differentiated sarcoma (2 cases), chromophobe renal cell carcinoma coexisting with liposarcoma (1 case), fibrosarcoma (1 case), embryonic sarcoma (1 case) and leiomyosarcoma (1 case). One patient died of tumor thrombus of the inferior vena cava during surgery. Finally, 15 cases were regularly followed up for 4 to 60 months. Till now, 1 had tumor-free survival for 9 months, and the other 14 cases died 2-38 months after the operation with a median survival time of 18 (range, 5-60) months. The median survival time of leiomyosarcoma group was 28 (range, 11-60) months, and 2 cases of malignant fibrous histiocytoma died 4 and 8 months after the operation respectively. Conclusions The primary renal sarcoma has the clinical symptom similar with advanced renal cell carcinoma and has poor prognosis. Leiomyosarcoma might have relative good prognosis.Keywords：renal sarcoma;adult;pathology4|2400|0Updated：2024-04-10
- Abstract：Objective To elucidate the effects of the deleted in colorectal carcinoma (DCC) gene on prolifera-tion of ovarian cancer cell line SKOV-3. Method An exogenous recombinant eukaryotic expression vector pcDNA3.1(+)-DCC, containing human DCC cDNA coding sequences, was constructed and transfected into SKOV-3 cells (SKOV-3/DCC). The pcDNA3.1 (+) transfected cells (SKOV-3/Neo) and SKOV-3 cells were used as the positive and nega-tive controls, respectively. Expressions of DCC mRNA and protein were analyzed by RT-PCR and immuno-cytochemical analysis, respectively. Cell growth was detected by soft agar colony formation assay and MTT assay. Flow cytometry and transmission electron microscopy were used to assess the effects of DCC on cell cycle distribution and ultrastructure, respectively. BALB/c mice were used to evaluate the effects of DCC on tumorigenicity in vivo. Results RT-PCR and immunocytochemical analysis revealed the exogenous DCC gene was suc-cessfully transfected into SKOV-3 cell lines and obtained permanent expression. The half maximal inhibitory concentration (IC50) of SKOV-3/DCC cells was significantly lower than that of SKOV-3 or SKOV-3/Neo cells (all P<0.05). DCC expression caused SKOV-3 cells to be arrested in G1 phase (78.0%), and electron microscopic analysis showed SKOV-3/DCC cells displayed typical morphological changes of apoptosis. Two mice xenografted with SKOV-3/DCC cells showed no tumor tumorigenecity. The tumor volume of BALB/c mice bearing SKOV-3/DCC cells (3.403 mm3) was smaller than that of SKOV-3 cells (9.206 mm3). Conclusion DCC gene may play an important role in suppressing the growth of SKOV-3 cell line and inducing apoptosis.Keywords：ovarian cancer;deleted in colorectal carcinoma gene;gene therapy4|2411|0Updated：2024-04-10
- Abstract：Objective To explore the feasibility and efficiency of a novel magnetic compression anastomats (MCAs) in intestinal anastomosis. Methods A total of 36 male mongrel canines underwent intestinal anastomosis using traditional hand-sewn (n=18) or a novel MCAs (n=18). We compared the anastomosis time, postoperative complica-tions, bursting strength of anastomoses, gross appearance, and pathology between two groups at each time- point of follow-up. Results The mean anastomosis time with MCAs was significantly less than that with hand-sewn (8.50±1.95 vs. 31.1±4.32 minutes, P<0.001). The blood stools and intussusceptions occurred in both groups during follow-up period. Only 1 mongrel canine receiving intestinal anastomosis by MCAs experi-enced anastomotic leakage. The average bursting pressure of anastomoses obtained from mongrel canines undergoing intestinal anastomosis by MCAs was significantly higher than that by traditional hand-sewn at 1 week’s follow-up time (P<0.05). Gross appearance of the anastomoses constructed by MCAs was relatively smoother and flatter. Pathological evalution of anastomoses revealed that general inflammation was greater in hand-sewn anastomoses than magnetic anastomosis. Conclusion The magnetic compression anastomat is a safe and effective device of sutureless intesti-nal anastomosis in canine models.Keywords：magnetic compression anastomosis;bursting strength;foreign body reaction4|3222|9Updated：2024-04-10
• Original Article •
- Abstract：To assess the time course of Q value after myopic laser-assisted in situ keratomileusis (LASIK) and preliminarily evaluate the determinants of the difference of Q value between before and after LASIK. We performed a retrospective, longitudinal investigation on patients undergoing wavefront optimized LASIK therapy for emmetropization. A total of 418 eyes from 222 cases were examined preoperatively, and partly followed up at one week (172 eyes), one month (134 eyes) and three months (51 eyes) after surgery. The horizontal, vertical and total Q values of cornea were calculated from eccentricity measured at the central 6-mm corneal zones respectively. Potential determinants of the change of Q value were analyzed using multiple linear regressions. The mean Q value was -0.17±0.13 preoperatively, and 0.99±0.70, 0.97±0.66, and 0.86±0.41 one week, one and three months postoperatively, respectively. One way analysis of variance (ANOVA) demonstrated significant differences between measurements made before surgery and at all postoperative times (at one week, one and three months; all P<0.0001, Bonferroni post hoc), but no significant differences were found among postoperative groups. Significant differences of Q values between horizontal and vertical meridians were found before surgery and at all postoperative times (all P<0.0001). Multiple regression analysis revealed that change of Q value significantly correlated with manifest refraction spherical equivalent (r=0.116, P<0.0001) and axial length (r=0.264, P<0.0001). Over the study period, the primary changes in Q value occur within 1 week after surgery, and then become slightly decreased and nearly stable. Manifest refraction spherical equivalent and axial length play a significant role in the change of postoperative Q value.Keywords：corneal asphericity;Q value;myopia;laser-assisted in situ keratomileusis4|2053|0Updated：2024-04-10
- Abstract：To assess the prevalence of anti-alpha-enolase antibody in systemic autoimmune diseases in Chinese patients and its role in endothelial cell apoptosis. The reactivity of anti-alpha-enolase antibody in a variety of autoimmune disorders in Chinese patients was evaluated by dot blot assay. Endothelial cell apoptosis was investigated by in vitro incubation of endothelial cells with IgG purified from anti-alpha-enolase antibody-positive sera, with or without pre-incubation with recombinant alpha-enolase. Anti-alpha-enolase antibody was prevalent in different systemic autoimmune diseases with relatively high reactivity in Chinese patients. In vitro incubation of endothelial cells with IgG containing anti-alpha-enolase antibody induced apoptosis in a time- and dose-dependent manner. Apoptosis was partly inhibited by pre-incubation of the endothelial cells with recombinant alpha-enolase. Our data suggest that alpha-enolase is a common auto-antigen recognized by anti- endothelial cell antibodies in connective tissue disease. Interaction between alpha-enolase and its autoantibody plays a role in endothelial cell apoptosis. Changes other than cell killing may contribute to the pathogenesis of endothelial damage and microvascular lesions.Keywords：anti-alpha-enolase antibody;apoptosis;endothelial cell4|2841|3Updated：2024-04-10
- Abstract：To explore the feasibility and efficiency of a novel magnetic compression anastomats (MCAs) in intestinal anastomosis. A total of 36 male mongrel canines underwent intestinal anastomosis using traditional hand-sewn (n=18) or a novel MCAs (n=18). We compared the anastomosis time, postoperative complica-tions, bursting strength of anastomoses, gross appearance, and pathology between two groups at each time- point of follow-up. The mean anastomosis time with MCAs was significantly less than that with hand-sewn (8.50±1.95 vs. 31.1±4.32 minutes, P<0.001). The blood stools and intussusceptions occurred in both groups during follow-up period. Only 1 mongrel canine receiving intestinal anastomosis by MCAs experi-enced anastomotic leakage. The average bursting pressure of anastomoses obtained from mongrel canines undergoing intestinal anastomosis by MCAs was significantly higher than that by traditional hand-sewn at 1 week’s follow-up time (P<0.05). Gross appearance of the anastomoses constructed by MCAs was relatively smoother and flatter. Pathological evalution of anastomoses revealed that general inflammation was greater in hand-sewn anastomoses than magnetic anastomosis. The magnetic compression anastomat is a safe and effective device of sutureless intesti-nal anastomosis in canine models.Keywords：magnetic compression anastomosis;bursting strength;foreign body reaction4|3258|9Updated：2024-04-10
- Keywords：sclerosing cholangitis;transcatheter arterial chemoembolization;bile duct;complication;treatment4|3571|0Updated：2024-04-10
- Keywords：choroidal tuberculoma;tuberculosis;choroiditis4|2387|0Updated：2024-04-10
- Keywords：amyloidosis;bladder;renal pelvis4|4528|0Updated：2024-04-10
• Case Report •
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