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2012 Year 27 Volume 2 Issue
- Abstract：Objective To investigate the expression profile of microRNA-21 in human cholangiocarcinoma tissues and to validate its bona fide targets in human cholangiocarcinoma cells. Methods The expression profile of microRNA-21 in human cholangiocarcinoma tissues and cholangiocarcinoma cell line, QBC939, was evaluated by using real-time PCR analysis. The bona fide targets of microRNA-21 were analyzed and confirmed by dual luciferase reporter gene assay and western blot, respectively. The expressional correlation of microRNA-21 and its targets was probed in human cholangiocarcinoma tissues by using real-time PCR, locked nucleic acid in situ hybridization (LNA-ISH), and immunohistochemistry analysis. Results Real-time PCR analysis revealed that microRNA-21 expression depicted a significant up-regulation in human cholangiocarcinoma tissues about 5.6-fold as compared to the matched normal bile Conclusion microRNA-21 expression is up-regulated in human cholangiocarcinoma and PTEN, PDCD4 are direct effectors of microRNA-21.Keywords：cholangiocarcinoma;microRNA-21;phosphatase and tensin homolog;programmed cell death 44|2544|4Updated：2024-04-10
- Abstract：Objective To investigate the main proteinases responsible for CD16b shedding under different stimulators. Methods HEK293 cell line stably expressing CD16b was constructed by lentivirus system. The cell line was then overexpressed with a disintegrin and metalloproteinase 10 (ADAM10) or ADAM17, suppressed with short hairpin RNA of ADAM10 or ADAM17, and reconstituted with ADAM10 or ADAM17, respectively. After each treatment, the cell line was stimulated with ionomycin or phorbol 12-myristate- 13-acetate (PMA) for 12 hours. The soluble CD16b released from cell membrane was detected by immunoprecipition and immunoblot. Quantitation was then implemented to compare the amount of soluble CD16b in cell supernatant after stimulation. Results HEK293 cell line stably expressing CD16b was successfully established. When CD16b expressing cell line was overexpressed with ADAM10, shedding of CD16b was increased after stimulation with ionomycin but not PMA; when the cell line overexpressed with ADAM17, shedding of CD16b was increased after stimulation with PMA but not ionomycin. Similarly, when ADAM10 was suppressed by short hairpin RNA, CD16b shedding was decreased after stimulation with ionomycin; when ADAM17 was suppressed by short hairpin RNA, CD16b shedding was decreased after stimulation with PMA. The shedding of CD16b was increased again when CD16b expressing cell line was reconstituted with ADAM10 and stimulated by ionomycin or reconstituted with ADAM17 and stimulated by PMA. Conclusions Both ADAM10 and ADAM17 could shed CD16b, but they possess differed preferences. ADAM10 is the main sheddase under stimulation of ionomycin, while ADAM17 is the main sheddase under stimulation of PMA.Keywords：a disintegrin and metalloproteinase 10;a disintegrin and metalloproteinase 17;CD16b;shedding4|3433|0Updated：2024-04-10
- Abstract：Objective To investigate the alterations of cardiac electrophysiological properties and substantial mechanism and find the stable arrhythmia mouse model in Kunming (KM) and C57BL6/J (C57) mice.Methods Electrocardiogram recordings were used to analyze the QT interval in vivo, and mono- phasic action potential of right and left ventricular epicardium was recorded to elicit changes of action potential duration (APD) in conventional and programmed electrical stimulation (PES). Transient outward potassium current (Ito) was recorded via whole-cell patch-clamp technique in single right and left epicardial myocytes.Results QT interval was prolonged in KM mice relative to C57 mice (62.51?4.47 ms vs. 52.59?4.85 ms, P<0.05). The APD at 50% repolarization of the left ventricular epicardium (18.60?0.91 ms vs. 12.90?0.35 ms), and APDs at 50% (17.31?6.05 ms vs. 12.00?3.24 ms) and 70% repolarization (36.13?5.32 ms vs. 21.95?8.06 ms) of the right ventricular epicardium in KM mice were significantly prolonged compared with C57 mice, respectively (all P<0.05). KM mice were more sensitive to PES-induced ventricular tachycardia (25%, 3 of 12 hearts), and especially to Burst-induced ventricular tachycardia (50%, 6 of 12 hearts) compared with C57 mice, which were 20% (2 of 10 hearts) and 30% (3 of 10 hearts) respectively. Ito densities both in the left and right ventricular epicardial myocytes from KM mice were significantly decreased compared with C57 mice, respectively (all P<0.01). Conclusion Our data showed that KM mice with the prolonged QT interval and APD are vulnerabilities to ventricular arrhythmia, which are attributed to lower Ito densities in ventricular myocytes obtained from KM mice than that from C57 mice.Keywords：cardiac electrophysiology;QT interval;action potential duration;transient outward potassium current;Kunming mouse;C57BL6/J mouse;arrhythmia4|3500|0Updated：2024-04-10
- Abstract：Objective To explore the correlations between serum uric acid (UA) levels and the clinical and cerebrospinal fluid (CSF) parameters of multiple sclerosis (MS). Methods The medical reports of 47 MS patients admitted to Peking Union Medical College Hospital during 2008 and 2010 were reviewed. And 49 age- and gender-matched cerebral infarction patients were enrolled as control. The mean serum UA level of the MS patients was compared with that of the control group. The correlations between the UA levels and the clinical parameters including gender, disease duration, relapse rate, and disease disabilities as assessed by the Expanded Disability Status Scale score, were explored. Forty-one patients had CSF examinations. The correlations between the UA levels and the CSF parameters reflecting inflammation and tissue damage, including CSF protein, white blood cell count, oligoclonal band, 24-hour IgG index, and myelin basic protein, were also investigated. Results The mean serum UA level in the MS patients was lower than that in the control group (247.75 ± 52.59 µmol/L vs. 277.94 ± 74.33 µmol/L, P=0.025) and inversely correlated with the relapse rate (P=0.049). MS patients with lower serum UA levels tended to have higher white blood cell counts and myelin basic protein level. But there was no correlation between CSF protein levels (r=0.165, P=0.273), white blood cell counts (r=-0.051, P=0.732), IgG index (r =0. 045, P=0.802), or myelin basic protein level (r =-0.248, P=0.145) and the serum UA level, respectively. Conclusion In MS patients, UA levels might partly reflect the extent of disability and inflammation.Keywords：uric acid;multiple sclerosis;relapse rate;cerebrospinal fluid4|3589|1Updated：2024-04-10
- Abstract：Objective To determine the safety and efficacy of fresh frozen plasma (FFP) infusion for the treatment of hereditary angioedema (HAE). Methods The medical records of patients with HAE admitted to Peking Union Medical College Hospital who had received FFP infusion during 2004 and 2010 were reviewed and PubMed database from 1966 to the present were searched using the following hereditary angioedema and fresh frozen plasma. The patient’s age, sex, body location of HAE attacks, the dose of FFP infusion, time of beginning to improvement, time to complete remission, complication, C1 inhibitor activity, and outcome were analyzed.Results A total of 13 enrolled patients (7 male and 6 female) received 16 times of FFP infusion, including 2 patients undergoing FFP infusion in Peking Union Medical College Hospital and 11 patients reported in the literature. The mean dosage of FFP infusion was 586±337 mL. Two cases suffered from worsening abdominal pain and one case experienced skin rash. Only 1 patient had no improvement in symptom owing to transfusion related reaction. There was a definite improvement in symptom 49±19 minutes after beginning FFP infusion. The remission time decreased from 61.7±27.0 hours to 3.3 (2.0, 12.0) hours after FFP infusion. FFP infusion was effective for both type Ⅰand type Ⅱ HAE.Conclusion FFP seems to be safe and effective for acute attacks of HAE.Keywords：hereditary angioedema;acute attacks;fresh frozen plasma;treatment4|4897|0Updated：2024-04-10
- Abstract：Objective To evaluate the safety and efficiency of hemi-semi laminectomy approach for the microsurgical treatment of spinal schwannomas. Methods A total of 22 patients underwent hemi-semi laminectomy for the microsurgical removal of spinal schwannomas during a period of 2009 and 2011 in Affiliated Hospital of Nantong University. We retrospectively analyzed the clinical outcomes of these patients. Results Of them, 5 cases were diagnosed with cervical schwannomas, 9 with thoracic schwannomas, and 8 with lumbar schwannomas. All the tumors including two dumbbell schwannomas were totally removed without major complications. Postoperatively, all patients were followed up from 6 to 36 months. The symptoms and signs were obviously improved, and no tumor recurrence or spinal deformity occurred. Conclusion Hemi-semi laminectomy is a safe and effective method for resection of spinal schwannomas.Keywords：spinal tumor;schwannomas;microsurgery;laminectomy4|5484|0Updated：2024-04-10
- Abstract：Objective To retrospectively investigate the clinical characteristics of sternal insufficiency fractures (SIFs) of post-menopausal women. Methods Findings on the clinical presentation, associated diseases, and imaging of SIFs in 17 postmenopausal women admitted to our hospital between February 1999 and January 2009 were reported. Results Twelve patients complained of severe pain in their anterior chest. Other symptoms included cough (5 cases), dyspnoea (3 cases), breathlessness (3 cases), and wheeze (2 cases). Four patients had no discomfort. The sternums of 11 cases were tender to palpation. Seventeen patients had osteoporosis. Other associated diseases were chronic obstructive pulmonary disease (7 cases), rheumatoid arthritis (3 cases), systemic lupus erythematosus (1 case), asthma (1 case), and thoracic vertebral fracture (13 cases). Nine patients had received glucocorticoid treatment. The fractures were located in the body of the sternum in 15 patients, in the manubrium in 1 patient, and in the manubriosternal junction in 1 patient. Displaced fracture was present in 13 cases. Lateral radiography of the sternum showed a fracture line in 14 patients. In the remaining 3 cases, other imaging examinations such as bone scan, computed tomography or magnetic resonance imaging demonstrated the presence of a fracture. Conclusions Osteoporosis, glucocorticoid therapy, chronic obstructive pulmonary disease, and rheumatoid arthritis might be risk factors for SIFs. SIFs should be considered in the differential diagnosis of chest pain.Keywords：sternum;insufficiency fracture;post-menopause;osteoporosis4|3511|0Updated：2024-04-10
- Abstract：Objective To investigate whether multiple coatings can improve the bond durability of one-step self-etching adhesive to primary dentin.Methods Twelve caries-free human primary molars were randomly divided into 2 groups. In group 1, each tooth was hemisected into 2 halves. One half was assigned to the control subgroup 1, which was bonded with a commercially available one-step self-etching adhesive according to the manufacturer’s instructions; the other half was assigned to experimental subgroup 1, in which the adhesive was applied three times before light curing. In group 2, one split half tooth was bonded with a commercially available one-step self-etching adhesive according to the manufacturer’s instructions; for the other half, three layers of adhesive were applied with each successive layer of light curing. Specimens were stored in 0.9% NaCl containing 0.02% sodium azide at 37℃ for 18 months and then were subjected to microtensile bond strength test and the fracture mode analysis. Results When the adhesive was applied three times before light curing, the bond strength of the experimental subgroup 1 was significantly higher than that of the control subgroup 1 (47.46±13.91 vs. 38.12±11.21 MPa, P0.05). Conclusion Multiple coatings can improve the bond durability of one-step self-etching adhesive to primary dentin when using the technique of light-curing after applying 3 layers of adhesive.Keywords：self-etching adhesive;microtensile bond strength test;primary dentin;multiple coatings4|3265|0Updated：2024-04-10
- Abstract：Objective To retrospectively investigate the distribution in kidney transplantation for fifteen years in Peking Union Medical College Hospital.Methods We conducted a descriptive research counting up the number of patients who received kidney transplantation each year in our hospital during 1995 and 2010.Results The first kidney transplantation in our hospital occurred in the 1960s. The number of kidney transplantation increased until reaching a maximum of 47 grafts in 2001; since then the number fell.Conclusions With the decreased number of kidney transplantation, we have realized the shortage of transplantable organs is very serious. The continuing transplant shortage requires major efforts to expand the donor pool. Donation after cardiac death offers the potential to enlarge the donor pool, but we need to strictly control the criteria for potential donors.Keywords：organ transplantation;kidney transplantation;donor crisis4|3534|0Updated：2024-04-10
- Abstract：LIMAS and Tio1 proposed the term meningeal melanocytoma first time in 1972 to describe a primary melanotic tumor of the leptomeninges with prolonged clinical course and benign histology. Meningeal melanocytoma of the central nervous system is rare and benign primary meningeal melanocytoma (PMM) is more exceptional, and also less usual than the malignant types.2 This rare tumor falls under the subclassification of primary melanocytic lesions in the World Health Organization’s classification of central nervous system tumors.3 PMM located in the foramen magnum region is an unusual cause of bulbus medullae and fourth ventricle compression. Here we report a 48-year-old man with a PMM which is located in the foramen magnum inducing supratentorial obstructive hydrocephalus.Keywords：foramen magnum;meningeal melanocytoma;histopathology;neuroimaging;obstructive hydrocephalus4|3694|0Updated：2024-04-10
- Abstract：METASTATIC gastric tumors are clinically uncommon, and metastatic gastric cancer from breast cancer was rarely reported. Here we report a case of gastric metastasis of atypical medullary carcinoma from breast.Keywords：gastric metastasis;breast cancer;atypical medullary carcinoma4|3389|0Updated：2024-04-10
• Original Article •
- Abstract：To investigate the expression profile of microRNA-21 in human cholangiocarcinoma tissues and to validate its bona fide targets in human cholangiocarcinoma cells. The expression profile of microRNA-21 in human cholangiocarcinoma tissues and cholangiocarcinoma cell line, QBC939, was evaluated by using real-time PCR analysis. The bona fide targets of microRNA-21 were analyzed and confirmed by dual luciferase reporter gene assay and western blot, respectively. The expressional correlation of microRNA-21 and its targets was probed in human cholangiocarcinoma tissues by using real-time PCR, locked nucleic acid hybridization (LNA-ISH), and immunohistochemistry analysis. Real-time PCR analysis revealed that microRNA-21 expression depicted a significant up-regulation in human cholangiocarcinoma tissues about 5.6-fold as compared to the matched normal bile Conclusion microRNA-21 expression is up-regulated in human cholangiocarcinoma and PTEN, PDCD4 are direct effectors of microRNA-21. △Supported by Institute of Basic Medical Sciences (2009PY13 and 2010PYZ18) and the National Natural Science Foundation of China (81100608 and 30901342).Keywords：cholangiocarcinoma;microRNA-21;phosphatase and tensin homolog;programmed cell death 44|4031|4Updated：2024-04-10
- Abstract：PRIMARY benign ureteral tumors are rare and they arise from the mesodermal tissue of the ureteral wall. Less than 200 cases have been docu- mented in published reports.1 Bilateral ureteral fibroepithelial polyps are extremely rare and commonly occur in the proximal ?ureter, causing complete ureteral obstruction. Pyeloplasty is an effective treatment for improving renal function. Here we report a case with sequential bilateral ureteral fibroepithelial polyps causing obstructive hydronephrosis.Keywords：ureteral polyps;fibroepithelial polyps4|3059|0Updated：2024-04-10
• Case Report •
- Abstract：MICRORNAs (miRNAs) are short (20-22 nucleotides), non-coding RNA molecules involved in the regulation of mRNAs by pairing with their 3'-untranslated region (3'-UTR) and affecting their stability or directing their silencing or degradation.1 miRNAs are likely to play roles in most cellular processes, including proliferation, development, differentiation, and apoptosis.2 People have made progress in the research of miRNA? field, but the study about microRNA-346 (miR-346) is very little. This study used biological software to analyze the biological characters of miR-346, and further reviewed the function of miR-346 and its roles in human diseases.4|3151|0Updated：2024-04-10
• Short Communication •
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