PTEN and PDCD4 are Bona Fide Targets of microRNA-21 in Human Cholangiocarcinoma△

Chinese Medical Sciences Journal ›› 2012, Vol. 27 ›› Issue (2): 65-72.

• Original Article • 下一篇

PTEN and PDCD4 are Bona Fide Targets of microRNA-21 in Human Cholangiocarcinoma^△

Chang-zheng Liu^1†*, Wei Liu^2†*, Yi Zheng², Jin-mei Su³, Jing-jing Li², Lan Yu², Xiao-dong He², and Song-sen Chen¹*

¹Department of Biochemistry, National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China
²Department of General Surgery, ³Department of Rheumatology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, China

收稿日期:2012-07-05 修回日期:2012-07-05 出版日期:2012-07-05 发布日期:2012-07-05
作者简介:†These authors equally contribute to this work.
*Corresponding author Chang-zheng Liu, Tel: 86-10-69156424, Fax: 86-10-65253005, E-mail: cz-liu@ibms.pumc.edu.cn; Song-sen Chen, Tel: 86-10-69156424, Fax: 86-10-65253005, E-mail: chen_ss@263.net

PTEN and PDCD4 are Bona Fide Targets of microRNA-21 in Human Cholangiocarcinoma

Chang-zheng Liu^1†*, Wei Liu^2†*, Yi Zheng², Jin-mei Su³, Jing-jing Li², Lan Yu², Xiao-dong He², and Song-sen Chen¹*

¹Department of Biochemistry, National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China
²Department of General Surgery, ³Department of Rheumatology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, China

Received:2012-07-05 Revised:2012-07-05 Published:2012-07-05 Online:2012-07-05

摘要/Abstract

摘要： Objective To investigate the expression profile of microRNA-21 in human cholangiocarcinoma tissues and to validate its bona fide targets in human cholangiocarcinoma cells.
Methods The expression profile of microRNA-21 in human cholangiocarcinoma tissues and cholangiocarcinoma cell line, QBC939, was evaluated by using real-time PCR analysis. The bona fide targets of microRNA-21 were analyzed and confirmed by dual luciferase reporter gene assay and western blot, respectively. The expressional correlation of microRNA-21 and its targets was probed in human cholangiocarcinoma tissues by using real-time PCR, locked nucleic acid in situ hybridization (LNA-ISH), and immunohistochemistry analysis.
Results Real-time PCR analysis revealed that microRNA-21 expression depicted a significant up-regulation in human cholangiocarcinoma tissues about 5.6-fold as compared to the matched normal bile
Conclusion microRNA-21 expression is up-regulated in human cholangiocarcinoma and PTEN, PDCD4 are direct effectors of microRNA-21.

关键词: cholangiocarcinoma, microRNA-21, phosphatase and tensin homolog, programmed cell death 4

Abstract: Objective To investigate the expression profile of microRNA-21 in human cholangiocarcinoma tissues and to validate its bona fide targets in human cholangiocarcinoma cells.
Methods The expression profile of microRNA-21 in human cholangiocarcinoma tissues and cholangiocarcinoma cell line, QBC939, was evaluated by using real-time PCR analysis. The bona fide targets of microRNA-21 were analyzed and confirmed by dual luciferase reporter gene assay and western blot, respectively. The expressional correlation of microRNA-21 and its targets was probed in human cholangiocarcinoma tissues by using real-time PCR, locked nucleic acid in situ hybridization (LNA-ISH), and immunohistochemistry analysis.
Results Real-time PCR analysis revealed that microRNA-21 expression depicted a significant up-regulation in human cholangiocarcinoma tissues about 5.6-fold as compared to the matched normal bile
Conclusion microRNA-21 expression is up-regulated in human cholangiocarcinoma and PTEN, PDCD4 are direct effectors of microRNA-21.

Key words: cholangiocarcinoma, microRNA-21, phosphatase and tensin homolog, programmed cell death 4

基金资助:

^△Supported by Institute of Basic Medical Sciences (2009PY13 and 2010PYZ18) and the National Natural Science Foundation of China (81100608 and 30901342).

Chang-zheng Liu, Wei Liu, Yi Zheng, Jin-mei Su, Jing-jing Li, Lan Yu, Xiao-dong He, and Song-sen Chen. PTEN and PDCD4 are Bona Fide Targets of microRNA-21 in Human Cholangiocarcinoma[J].Chinese Medical Sciences Journal, 2012, 27(2): 65-72.

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PTEN and PDCD4 are Bona Fide Targets of microRNA-21 in Human Cholangiocarcinoma^△

PTEN and PDCD4 are Bona Fide Targets of microRNA-21 in Human Cholangiocarcinoma

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