Regulation of acyl-coenzyme A: cholesterol acyltransferase 2 expression by saturated fatty acids

doi:10.1016/S1001-9294(11)60006-0

Chinese Medical Sciences Journal ›› 2010, Vol. 25 ›› Issue (4): 222-227.doi: 10.1016/S1001-9294(11)60006-0

• Original Article • 上一篇下一篇

Regulation of acyl-coenzyme A: cholesterol acyltransferase 2 expression by saturated fatty acids

Zhu-qin Zhang, Hou-zao Chen, Rui-feng Yang, Ran Zhang, Yu-yan Jia, Yang Xi, De-pei Liu*, and Chih-chuan Liang#

National Laboratory of Medical Molecular Biology, Institute of Basic Medical Science, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China

收稿日期:2010-10-29 出版日期:2010-12-20 发布日期:2010-12-20
通讯作者: *Correspondingauthor Tel: 86-10-65296415, Fax: 86-10-65105093, E-mail: liudp@pumc.edu.cn E-mail:liudp@pumc.edu.cn

Regulation of acyl-coenzyme A: cholesterol acyltransferase 2 expression by saturated fatty acids

Zhu-qin Zhang, Hou-zao Chen,Rui-feng Yang, Ran Zhang,Yu-yan Jia, Yang Xi, De-pei Liu*, and Chih-chuan Liang^#

National Laboratory of Medical Molecular Biology, Institute of Basic Medical Science, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China

Received:2010-10-29 Online:2010-12-20 Published:2010-12-20
Contact: National Laboratory of Medical Molecular Biology, Instituteof BasicMedical Science, Chinese Academyof Medical Sciences &Peking Union Medical College,Beijing 100005, China E-mail:liudp@pumc.edu.cn

摘要/Abstract

摘要： Objective To verify the regulation of acyl-coenzyme A:cholesterol acyltransferase 2 (ACAT 2), which is associated with cholesterol metabolism, by saturated fatty acids (SFAs). Methods Palmitic acid (PA), the most abundant saturated fatty acid in plasma, and oleic acid (OA), a widely distributed unsaturated fatty acid, were used to treat hepatic cells HepG2, HuH7, and mouse primary hepatocytes. In addition, PA at different concentrations and PA treatment at different durations were applied in HepG2 cells. In in vivo experiment, three-month male C57/BL6 mice were fed with control diet and SFA diet containing hydrogenated coconut oil rich of SFAs. The mRNA level of ACAT2 in those hepatic cells and the mouse livers was detected with real-time polymerase chain reaction (PCR). Results In the three types of hepatic cells treated with PA, that SFA induced significant increase of ACAT2 expression (P＜0.01), whereas treatment with OA showed no significant effect. That effect of PA was noticed gradually rising along with the increase of PA concentration and the extension of PA treatment duration (both P＜0.05). SFA diet feeding in mice resulted in a short-term and transient increase of ACAT2 expression in vivo, with a peak level appearing in the mice fed with SFA diet for two days (P＜0.05). Conclusion SFA may regulate ACAT2 expression in human and mouse hepatic cells and in mouse livers.

关键词: acyl-coenzyme A:cholesterol acyltransferase 2, gene expression, saturatedfatty acid

Abstract: Objective To verify the regulation of acyl-coenzyme A:cholesterol acyltransferase 2 (ACAT 2), which is associated with cholesterol metabolism, by saturated fatty acids (SFAs). Methods Palmitic acid (PA), the most abundant saturated fatty acid in plasma, and oleic acid (OA), a widely distributed unsaturated fatty acid, were used to treat hepatic cells HepG2, HuH7, and mouse primary hepatocytes. In addition, PA at different concentrations and PA treatment at different durations were applied in HepG2 cells. In in vivo experiment, three-month male C57/BL6 mice were fed with control diet and SFA diet containing hydrogenated coconut oil rich of SFAs. The mRNA level of ACAT2 in those hepatic cells and the mouse livers was detected with real-time polymerase chain reaction (PCR). Results In the three types of hepatic cells treated with PA, that SFA induced significant increase of ACAT2 expression (P＜0.01), whereas treatment with OA showed no significant effect. That effect of PA was noticed gradually rising along with the increase of PA concentration and the extension of PA treatment duration (both P＜0.05). SFA diet feeding in mice resulted in a short-term and transient increase of ACAT2 expression in vivo, with a peak level appearing in the mice fed with SFA diet for two days (P＜0.05). Conclusion SFA may regulate ACAT2 expression in human and mouse hepatic cells and in mouse livers.

Key words: acyl-coenzyme A:cholesterol acyltransferase 2, gene expression, saturatedfatty acid

基金资助:

National Natural Science Foundation of China(30721063), National High Technology Research and Development Program of China(863 Program) (2006AA02A406), NationalBasic Research Program of China (973 Program) (2006CB503801), and Special Fund ofthe National Laboratory of China(2060204).

Zhu-qin Zhang, Hou-zao Chen, Rui-feng Yang, Ran Zhang, Yu-yan Jia, Yang Xi, De-pei Liu* and Chih-chuan Liang. Regulation of acyl-coenzyme A: cholesterol acyltransferase 2 expression by saturated fatty acids[J].Chinese Medical Sciences Journal, 2010, 25(4): 222-227.

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Regulation of acyl-coenzyme A: cholesterol acyltransferase 2 expression by saturated fatty acids

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