Oncogenic Role of Skp2 and p27Kip1 in Intraductal Proliferative Lesions of the Breast△

doi:10.1016/S1001-9294(14)60049-3

Chinese Medical Sciences Journal ›› 2012, Vol. 27 ›› Issue (3): 161-166.doi: 10.1016/S1001-9294(14)60049-3

• Original Article • 上一篇下一篇

Oncogenic Role of Skp2 and p27Kip1 in Intraductal Proliferative Lesions of the Breast△

Yan Lv¹?*, Yun Niu², Xiu-min Ding², Xu-qi Xiao²

1Department of Acupuncture and Moxibustion, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
2Breast Cancer Research and Pathology Laboratory, Cancer Institute & Hospital of Tianjin Medical University, Tianjin 300060, China

收稿日期:2012-04-19 修回日期:2012-10-08 出版日期:2012-10-08 发布日期:2012-10-08
作者简介:The author is now working in the Department of Pathology, First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China. *Corresponding author Tel: 86-22-27432212, E-mail: lvyanella@ 163.com

Oncogenic Role of Skp2 and p27Kip1 in Intraductal Proliferative Lesions of the Breast△

Yan Lv¹?*, Yun Niu², Xiu-min Ding², Xu-qi Xiao²

1Department of Acupuncture and Moxibustion, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
2Breast Cancer Research and Pathology Laboratory, Cancer Institute & Hospital of Tianjin Medical University, Tianjin 300060, China

Received:2012-04-19 Revised:2012-10-08 Published:2012-10-08 Online:2012-10-08
About author:The author is now working in the Department of Pathology, First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China. *Corresponding author Tel: 86-22-27432212, E-mail: lvyanella@ 163.com

摘要/Abstract

摘要： To investigate whether the connection of p27^Kip1 to S-phase kinase-associated protein 2 (Skp2) plays an oncogenic role in intraductal proliferative lesions of the breast.
Methods Here we investigated the mechanism involved in association of Skp2’s degradation of p27^Kip1 with the breast carcinogenesis by immunohistochemical method through detection of Skp2 and p27^Kip1 protein levels in 120 paraffin-embedded tissues of intraductal proliferative lesions including usual ductal hyperplasia (UDH, n=30), atypical ductal hyperplasia (n=30), flat epithelial atypia (FEA, n=30), and ductal carcinoma in situ (DCIS, n=30).Moreover, the expression status of Skp2 and p27^Kip1 in 30 cases of the normal breast paraffin-embedded tissues were explored.
Results The DCIS group was with the highest Skp2 level and the lowest p27^Kip1 level, and the UDH group was with the lowest Skp2 level and the highest p27^Kip1 level.Both Skp2 and p27^Kip1 levels in the DCIS group were significantly different from those in the UDH group (all P<0.01).The levels of Skp2 and p27^Kip1 in the FEA group were significantly different from both the DCIS and UDH groups (all P<0.05).p27^Kip1 was negatively correlated with Skp2 in both the UDH group (r=-0.629, P=0.026) and DCIS group (r=-0.893, P=0.000).
Conclusion Overexpression of Skp2 might be the mechanism underlying p27^Kip1 over degradation.

关键词: breast cancer, intraductal proliferative lesions, p27Kip1, S-phase kinase-associated protein 2

Abstract: To investigate whether the connection of p27^Kip1 to S-phase kinase-associated protein 2 (Skp2) plays an oncogenic role in intraductal proliferative lesions of the breast.
Methods Here we investigated the mechanism involved in association of Skp2’s degradation of p27^Kip1 with the breast carcinogenesis by immunohistochemical method through detection of Skp2 and p27^Kip1 protein levels in 120 paraffin-embedded tissues of intraductal proliferative lesions including usual ductal hyperplasia (UDH, n=30), atypical ductal hyperplasia (n=30), flat epithelial atypia (FEA, n=30), and ductal carcinoma in situ (DCIS, n=30).Moreover, the expression status of Skp2 and p27^Kip1 in 30 cases of the normal breast paraffin-embedded tissues were explored.
Results The DCIS group was with the highest Skp2 level and the lowest p27^Kip1 level, and the UDH group was with the lowest Skp2 level and the highest p27^Kip1 level.Both Skp2 and p27^Kip1 levels in the DCIS group were significantly different from those in the UDH group (all P<0.01).The levels of Skp2 and p27^Kip1 in the FEA group were significantly different from both the DCIS and UDH groups (all P<0.05).p27^Kip1 was negatively correlated with Skp2 in both the UDH group (r=-0.629, P=0.026) and DCIS group (r=-0.893, P=0.000).
Conclusion Overexpression of Skp2 might be the mechanism underlying p27^Kip1 over degradation.

Key words: breast cancer, intraductal proliferative lesions, p27Kip1, S-phase kinase-associated protein 2

基金资助:

△Supported by the National Natural Science Foundation of China (30471967) and research funding of Tianjin Cancer Institute & Hospital of Tianjin Medical University.

Yan Lv?*, Yun Niu, Xiu-min Ding, Xu-qi Xiao. Oncogenic Role of Skp2 and p27Kip1 in Intraductal Proliferative Lesions of the Breast△[J].Chinese Medical Sciences Journal, 2012, 27(3): 161-166.

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Oncogenic Role of Skp2 and p27Kip1 in Intraductal Proliferative Lesions of the Breast△

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