Lipopolysaccharide Challenge Induces Long Pentraxin 3 Expression in Mice Independently from Acute Lung Injury△

doi:10.1016/S1001-9294(15)30002-X

摘要/Abstract

摘要： Objective To determine whether the onset of acute lung injury (ALI) induces the up-regulation of pentraxin 3 (PTX3) expression in mice and whether PTX3 concentration in the biofluid can help recognizing sepsis-induced ALI.
Methods Wild-type C57BL/6 mice (12-14 weeks old) were randomly divided into 3 groups. Mice in the group 1 (n=12) and group 2 (n=12) were instilled with lipopolysaccharide via intratracheal or intraperitoneal routes, respectively. Mice in the group 3 (n=8) were taken as blank controls. Pulmonary morphological and functional alterations were measured to determine the presence of experimental ALI. PTX3 expression in the lung was quantified at both protein and mRNA levels. PTX3 protein concentration in blood and bronchoalveolar lavage fluid was measured to evaluate its ability to diagnose sepsis-induced ALI by computing area under receiver operator characteristic curve (AUROCC).
Results ALI was commonly confirmed in the group 1 but never in the other groups. PTX3 expression was up-regulated indiscriminately among lipopolysaccharide-challenged mice. PTX3 protein concentration in the biofluid was unable to diagnose sepsis-induced ALI evidenced by its small AUROCC. PTX3 concentration in bronchoalveolar lavage fluid did not correlate with that in serum.
Conclusions Lipopolysaccharide challenges induced PTX3 expression in mice regardless of the presence of ALI. PTX3 may act as an indicator of inflammatory response instead of organ injury per se.

关键词: long pentraxin 3, acute lung injury, biomarker, sepsis, lipopolysaccharide

Abstract: Objective To determine whether the onset of acute lung injury (ALI) induces the up-regulation of pentraxin 3 (PTX3) expression in mice and whether PTX3 concentration in the biofluid can help recognizing sepsis-induced ALI.
Methods Wild-type C57BL/6 mice (12-14 weeks old) were randomly divided into 3 groups. Mice in the group 1 (n=12) and group 2 (n=12) were instilled with lipopolysaccharide via intratracheal or intraperitoneal routes, respectively. Mice in the group 3 (n=8) were taken as blank controls. Pulmonary morphological and functional alterations were measured to determine the presence of experimental ALI. PTX3 expression in the lung was quantified at both protein and mRNA levels. PTX3 protein concentration in blood and bronchoalveolar lavage fluid was measured to evaluate its ability to diagnose sepsis-induced ALI by computing area under receiver operator characteristic curve (AUROCC).
Results ALI was commonly confirmed in the group 1 but never in the other groups. PTX3 expression was up-regulated indiscriminately among lipopolysaccharide-challenged mice. PTX3 protein concentration in the biofluid was unable to diagnose sepsis-induced ALI evidenced by its small AUROCC. PTX3 concentration in bronchoalveolar lavage fluid did not correlate with that in serum.
Conclusions Lipopolysaccharide challenges induced PTX3 expression in mice regardless of the presence of ALI. PTX3 may act as an indicator of inflammatory response instead of organ injury per se.

Key words: long pentraxin 3, acute lung injury, biomarker, sepsis, lipopolysaccharide

基金资助: △Partly supported by a grant from Jie-shou Li Academician Gut Barrier Research Fund

Gao Zeng, Jie Liu, Ning Wu, Cong-wei Jia, Shu-bin Guo. Lipopolysaccharide Challenge Induces Long Pentraxin 3 Expression in Mice Independently from Acute Lung Injury[J].Chinese Medical Sciences Journal, 2015, 30(1): 7-17.

参考文献 39

1.	Ware LB, Matthay MA. The acute respiratory distress syndrome. N Engl J Med 2000; 342:1334-49.
2.	Luh SP, Chiang CH. Acute lung injury/acute respiratory distress syndrome (ALI/ARDS): The mechanism, present strategies and future perspectives of therapies. J Zhejiang Univ Sci B 2007; 8:60-9.
3.	Janz DR, Ware LB. Biomarkers of ALI/ARDS: Patho- genesis, discovery, and relevance to clinical trials. Semin Respir Crit Care Med 2013; 34:537-48.
4.	Bhargava M, Wendt CH. Biomarkers in acute lung injury. Transl Res 2012; 159:205-17.
5.	Agrawal A, Singh PP, Bottazzi B, et al. Pattern recognition by pentraxins. Adv Exp Med Biol 2009; 653:98-116.
6.	He X, Han B, Liu M. Long pentraxin 3 in pulmonary infection and acute lung injury. Am J Physiol Lung Cell Mol Physiol 2007; 292:L1039-49.
7.	Han B, Mura M, Andrade CF, et al. TNFalpha-induced long pentraxin PTX3 expression in human lung epithelial cells via JNK. J Immunol 2005; 175:8303-11.
8.	dos Santos CC, Han B, Andrade CF, et al. DNA microarray analysis of gene expression in alveolar epithelial cells in response to TNFalpha, LPS, and cyclic stretch. Physiol Genomics 2004; 19:331-42.
9.	He X, Han B, Bai X, et al. PTX3 as a potential biomarker of acute lung injury: Supporting evidence from animal experimentation. Intensive Care Med 2010; 36:356-64.
10	Okutani D, Han B, Mura M, et al. High-volume ventilation induces pentraxin 3 expression in multiple acute lung injury models in rats. Am J Physiol Lung Cell Mol Physiol 2007; 292:L144-53.
11	Dias AA, Goodman AR, Dos Santos JL, et al. TSG-14 transgenic mice have improved survival to endotoxemia and to CLP-induced sepsis. J Leukoc Biol 2001; 69:928-36.
12	Han B, Haitsma JJ, Zhang Y, et al. Long pentraxin PTX3 deficiency worsens LPS-induced acute lung injury. Intensive Care Med 2011; 37:334-42.
13	Muller B, Peri G, Doni A, et al. Circulating levels of the long pentraxin PTX3 correlate with severity of infection in critically ill patients. Crit Care Med 2001; 29:1404-7.
14	Mauri T, Coppadoro A, Bellani G, et al. Pentraxin 3 in acute respiratory distress syndrome: An early marker of severity. Crit Care Med 2008; 36:2302-8.
15	Matute-Bello G, Downey G, Moore BB, et al. An official American Thoracic Society workshop report: Features and measurements of experimental acute lung injury in animals. Am J Respir Cell Mol Biol 2011; 44:725-38.
16	16.Remick DG, Bolgos GR, Siddiqui J, et al. Six at six: Interleukin-6 measured 6 hours after the initiation of sepsis predicts mortality over 3 days. Shock 2002; 17:463-7.
17	17.Osuchowski MF, Connett J, Welch K, et al. Stratification is the key: Inflammatory biomarkers accurately direct immunomodulatory therapy in experimental sepsis. Crit Care Med 2009; 37:1567-73.
18	18.Sevransky JE, Levy MM, Marini JJ. Mechanical ventilation in sepsis-induced acute lung injury/acute respiratory distress syndrome: An evidence-based review. Crit Care Med 2004; 32:S548-53.
19	19.Chen H, Bai C, Wang X. The value of the lipopolysaccharide- induced acute lung injury model in respiratory medicine. Expert Rev Resp Med 2010; 4:773-83.
20	20.Reiss LK, Uhlig U, Uhlig S. Models and mechanisms of acute lung injury caused by direct insults. Eur J Cell Biol 2012; 91:590-601.
21	21.Matute-Bello G, Frevert CW, Martin TR. Animal models of acute lung injury. Am J Physiol Lung Cell Mol Physiol 2008; 295:L379-99.
22	22.Sadowitz B, Roy S, Gatto LA, et al. Lung injury induced by sepsis: Lessons learned from large animal models and future directions for treatment. Expert Rev Anti Infect Ther 2011; 9:1169-78.
23	23.Szarka RJ, Wang N, Gordon L, et al. A murine model of pulmonary damage induced by lipopolysaccharide via intranasal instillation. J Immunol Methods 1997; 202:49-57.
24	24.van Helden HP, Kuijpers WC, Steenvoorden D, et al. Intratracheal aerosolization of endotoxin (LPS) in the rat: A comprehensive animal model to study adult (acute) respiratory distress syndrome. Exp Lung Res 1997; 23:297-316.
25	25.Li QF, Zhu YS, Jiang H, et al. Isoflurane preconditioning ameliorates endotoxin-induced acute lung injury and mortality in rats. Anesth Analg 2009; 109:1591-7.
26	26.Bashenko Y, Ilan N, Krausz MM, et al. Heparanase pretreatment attenuates endotoxin-induced acute lung injury in rats. Shock 2007; 28:207-12.
27	27.Nakajima T, Suarez CJ, Lin KW, et al. T cell pathways involving CTLA4 contribute to a model of acute lung injury. J Immunol 2010; 184:5835-41.
28	28.Perl M, Lomas-Neira J, Venet F, et al. Pathogenesis of indirect (secondary) acute lung injury. Expert Rev Respir Med 2011; 5:115-26.
29	29.Baruah P, Propato A, Dumitriu IE, et al. The pattern recognition receptor PTX3 is recruited at the synapse between dying and dendritic cells, and edits the cross-presentation of self, viral, and tumor antigens. Blood 2006; 107:151-8.
30	30.Souza DG, Soares AC, Pinho V, et al. Increased mortality and inflammation in tumor necrosis factor-stimulated gene-14 transgenic mice after ischemia and reperfusion injury. Am J Pathol 2002; 160:1755-65.
31	31.Biomarkers Definitions Working G. Biomarkers and surrogate endpoints: Preferred definitions and conceptual framework. Clin Pharmacol Ther 2001; 69:89-95.
32	32.Paragas N, Qiu A, Zhang Q, et al. The Ngal reporter mouse detects the response of the kidney to injury in real time. Nat Med 2011; 17:216-22.
33	33.Garlanda C, Bottazzi B, Bastone A, et al. Pentraxins at the crossroads between innate immunity, inflammation, matrix deposition, and female fertility. Annu Rev Immunol 2005; 23:337-66.
34	34.Breviario F, d'Aniello EM, Golay J, et al. Interleukin- 1-inducible genes in endothelial cells. Cloning of a new gene related to C-reactive protein and serum amyloid P component. J Biol Chem 1992; 267:22190-7.
35	35.Azzurri A, Sow OY, Amedei A, et al. IFN-gamma-inducible protein 10 and pentraxin 3 plasma levels are tools for monitoring inflammation and disease activity in Mycobacterium tuberculosis infection. Microbes Infect 2005; 7:1-8.
36	36.Latini R, Maggioni AP, Peri G, et al. Prognostic significance of the long pentraxin PTX3 in acute myocardial infarction. Circulation 2004; 110:2349-54.
37	37.Mairuhu AT, Peri G, Setiati TE, et al. Elevated plasma levels of the long pentraxin, pentraxin 3, in severe dengue virus infections. J Med Virol 2005; 76:547-52.
38	38.Bevelacqua V, Libra M, Mazzarino MC, et al. Long pentraxin 3: A marker of inflammation in untreated psoriatic patients. Int J Mol Med 2006; 18:415-23.
39	39.Inoue K, Sugiyama A, Reid PC, et al. Establishment of a high sensitivity plasma assay for human pentraxin3 as a marker for unstable angina pectoris. Arterioscler Thromb Vasc Biol 2007; 27:161-7.

[1]	Yan-feng Li, Fang-fang Ge, Yong Zhang, Hui You, Zhen-xin Zhang. Cerebrospinal Fluid Biomarkers in Dementia Patients with Cerebral Amyloid Angiopathy[J]. Chinese Medical Sciences Journal, 2015, 30(3): 170-173.
[2]	Gao Zeng, Cong-wei Jia, Jie Liu, Shu-bin Guo. Lipocalin-2 Test in Distinguishing Acute Lung Injury Cases from Septic Mice Without Acute Lung Injury^△[J]. Chinese Medical Sciences Journal, 2014, 29(2): 65-77.

优先出版

当期目次

过刊浏览

虚拟专题

作者中心

评阅者中心

Lipopolysaccharide Challenge Induces Long Pentraxin 3 Expression in Mice Independently from Acute Lung Injury^△

Lipopolysaccharide Challenge Induces Long Pentraxin 3 Expression in Mice Independently from Acute Lung Injury

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献 39

相关文章 2

Metrics

推荐阅读 6