Chinese Medical Sciences Journal ›› 2018, Vol. 33 ›› Issue (4): 240-251.doi: 10.24920/003483

• 综述 • 上一篇    下一篇

合成生物传感器在疾病检测领域的应用技术进展: 设计、分类和展望


  1. 精准外科与再生医学国家地方联合工程研究中心,陕西省再生医学与外科工程研究中心,先进外科技术与工程研究所,西安交通大学第一附属医院,西安,710061中国
  • 收稿日期:2018-06-07 接受日期:2018-08-17 出版日期:2018-12-30 发布日期:2018-10-08

Current Technologies of Synthetic Biosensors for Disease Detection: Design, Classification and Future Perspectives

Chen Xue,Lv Yi,Wu Rongqian   

  1. National-Local Joint Engineering Research Center for Precision Surgery & Regenerative Medicine, Shaanxi Provincial Center for Regenerative Medicine and Surgical Engineering, Institute of Advanced Surgical Technology and Engineering, the First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, 710061 China
  • Received:2018-06-07 Accepted:2018-08-17 Published:2018-12-30 Online:2018-10-08



关键词: 合成生物传感器, 基因线路, 疾病检测


Synthetic biology aims to endow living cells with new functions by incorporating functional gene networks into them. By overexpressing, blocking and rewiring native gene pathways, synthetic biologists have harnessed this promising technology to reprogram cells to perform diverse tasks such as drug discovery, biopharmaceutical manufacturing, gene therapy and tissue engineering, etc. In this review, we focus on current technologies of synthetic biosensors for disease detection. We start with the design principle of synthetic biosensors. Then we move towards the characteristics of simple synthetic biosensors, which can respond to a single input signal, and complex synthetic biosensors including Boolean gate biosensors, cascade biosensors, time-delay biosensors, oscillator biosensors and hysteretic biosensors, which can respond to more than two input signals and perform complex tasks. Synthetic biosensor has showed great potential in disease detection, but it is still in its infancy stage. More efforts should be made in identifying and constructing clinically relevant regulation systems. Computational tools are also needed in the design process in order to guarantee the precision of the synthetic biosensor. The ultimate goal of a synthetic biosensor is to act as a therapeutic sensor-effector device that connects diagnostic input with therapeutic output and therefore provides all-in-one diagnostic and therapeutic solutions for future gene- and cell-based therapies.

Key words: synthetic biosensor, gene circuit, disease detection

补充说明: 本文系统综述了不同类型合成生物传感器的设计原理以及在疾病诊疗方面的最新应用,包括免疫调控、肝损伤、胰岛素抵抗、Grave's病、以及尿酸内稳态等方面。

基金资助: 国家自然科学基金(81770491)

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