Chinese Medical Sciences Journal ›› 2010, Vol. 25 ›› Issue (2): 90-94.

• Original Article • 上一篇    下一篇

D-Tyr-tRNATyr Deacylase, a New Role in Alzheimer’s-associated Disease in SAMP8 Mice

Wei Liu1,2, Chang Liu1, Jing-xi Zhu1, Ai-hua Li1, Zhi-qiang Zhao1,Bin Yin1, and Xiao-zhong Peng1*   

  1. 1National Laboratory of Medical Molecular Biology, 2Department of Anatomy and Histology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China
  • 收稿日期:2010-05-14 出版日期:2010-06-10 发布日期:2010-06-10
  • 作者简介:Tel: 86-10-65296411, Fax: 86-10-65240529

D-Tyr-tRNATyr Deacylase, a New Role in Alzheimer’s-associated Disease in SAMP8 Mice

Wei Liu1,2, Chang Liu1, Jing-xi Zhu1, Ai-hua Li1, Zhi-qiang Zhao1,Bin Yin1, and Xiao-zhong Peng1*   

  1. 1National Laboratory of Medical Molecular Biology, 2Department of Anatomy and Histology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China
  • Received:2010-05-14 Online:2010-06-10 Published:2010-06-10
  • About author:Tel: 86-10-65296411, Fax: 86-10-65240529

摘要: Objective To assess the expression level of D-Tyr-tRNATyr deacylase (DTD) in SAMP8 mice and speculate the function of DTD in disorders associated with Alzheimer's disease (AD). Methods Altogether 12 SAMP8 mice and 12 SAMR1 mice were used in this study. Semi-quantita- tive reverse transcription-polymerase chain reaction (RT-PCR) and Western blot were performed to detect the mRNA and protein levels of DTD in the mice. Purified DTD protein was injected into lateral ventricle to investigate the function of DTD in SAMP mice. The behavior of the mice was tested by using a Step-through Test System. Results Both mRNA and protein levels of DTD were found to be significantly lower in SAMP8 mice compared with those in SAMR1 mice (P<0.05). In vivo injection of DTD protein did not lead to an obvious change in behavior of SAM mice. Conclusions DTD might function in the process of AD-associated pathology and could possibly participate in physiology process in a long-term manner to orchestrate with other regulators in order to maintain the balance of organism.

关键词: D-Tyr-tRNATyr deacylase, D-amino acid, SAMP8 mice, neurodegenerative disease

Abstract: Objective To assess the expression level of D-Tyr-tRNATyr deacylase (DTD) in SAMP8 mice and speculate the function of DTD in disorders associated with Alzheimer's disease (AD). Methods Altogether 12 SAMP8 mice and 12 SAMR1 mice were used in this study. Semi-quantita- tive reverse transcription-polymerase chain reaction (RT-PCR) and Western blot were performed to detect the mRNA and protein levels of DTD in the mice. Purified DTD protein was injected into lateral ventricle to investigate the function of DTD in SAMP mice. The behavior of the mice was tested by using a Step-through Test System. Results Both mRNA and protein levels of DTD were found to be significantly lower in SAMP8 mice compared with those in SAMR1 mice (P<0.05). In vivo injection of DTD protein did not lead to an obvious change in behavior of SAM mice. Conclusions DTD might function in the process of AD-associated pathology and could possibly participate in physiology process in a long-term manner to orchestrate with other regulators in order to maintain the balance of organism.

Key words: D-Tyr-tRNATyr deacylase, D-amino acid, SAMP8 mice, neurodegenerative disease

基金资助:

Supported by National Natural Science Foundation of China (30721063)

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