Chinese Medical Sciences Journal ›› 2010, Vol. 25 ›› Issue (2): 95-99.doi: 10.1016/S1001-9294(10)60029-6

• Original Article • Previous Articles     Next Articles

Gaussia Luciferase Reporter Assay for Assessment of Gene Delivery Systems in Vivo

Feng Chen, Zhen Xu, Jing Lu, Xiang Lv, Wen-li Mu, Ya-jun Wang, De-pei Liu*, and Chih-chuan Liang   

  1. National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China
  • Received:2010-05-07 Online:2010-06-10 Published:2010-06-10
  • About author:Tel: 86-10-65296415, Fax: 86-10-65105093
  • Supported by:

    Supported by National High Technology Research and De- velopment Program of China (863 Program) (2007AA021206, 2007AA021106)

Abstract: Objective To develop an alternative method for assessment of gene delivery systems in vivo. Methods Mouse primary spleen lymphocytes were genetically modified in vitro by a retroviral vector harboring a Gaussia luciferase (Gluc) expression cassette. After implantation of these cells into recipient mice, the expression of Gluc was detected in whole blood or plasma collected. Results As little as 10 uL whole blood drawn from the recipient mice could guarantee prompt reading of Gluc activity with a luminometer. And the reading was found in good correlation with the number of genetically modified spleen lymphocytes implanted to the mice. Conclusions Gluc may be useful as an in vivo reporter for gene therapy researches, and Gluc blood assay could provide an alternative method for assessment of gene delivery systems in vivo.

Key words: gene delivery systems, reporter, Gaussia luciferase

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