Chinese Medical Sciences Journal ›› 2011, Vol. 26 ›› Issue (4): 216-220.doi: 10.1016/S1001-9294(12)60003-0

• Original Article • Previous Articles     Next Articles

Contrary Regulation of TIMP-1 and MMP-9 by Hepatocyte Growth Factor Antibody after Lung Injury

Wei-wei Yu, and Qin Xia*   

  1. Institute of General Department, Tongji Hospital, Tongji Medical College, Huazhong;University of Science and Technology, Wuhan 430030, China
  • Online:2011-12-30 Published:2011-12-30
  • Contact: *Corresponding author Tel: 86-27-83663016, E-mail: yuweiwei75@ gmail.com E-mail:yuweiwei75@ gmail.com

Abstract: Objective To study the influence of hepatocyte growth factor (HGF) antibody on the lung expression level of matrix metalloproteinases-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1).
Methods Thirty male Wistar rats were randomly divided into 3 groups: control group, model group, and intervention group. Endotoxin was intratracheally infused in the model and intervention groups. HGF antibody was injected in the rats of the intervention group from day 1 to day 14, while the same volume of saline was injected in the control group. The rats were sacrificed on day 28 after endotoxin treatment. The amounts of MMP-9 mRNA and TIMP-1 mRNA were measured by reverse transcription-polymerase chain reaction, and protein expression levels of MMP-9 and TIMP-1 were measured by immunohistochem-istry.
Results In the model group, both mRNA and protein expression levels of TIMP-1 were significantly increased, the same as MMP-9. In the intervention group, the increase of TIMP-1 was remarkably reduced compared with the model group, while the mRNA and protein expression levels of MMP-9 were still increased.
Conclusion HGF activity may accelerate the repair of lung injury through contrary regulating the expression levels of TIMP-1 and MMP-9.

Key words: hepatocyte growth factor, matrix metalloproteinases-9, tissue inhibitor of metalloproteinases-1

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