Chinese Medical Sciences Journal ›› 2013, Vol. 28 ›› Issue (2): 88-94.doi: 10.1016/S1001-9294(13)60028-0

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Physiological Testosterone Retards Cardiomyocyte Aging in Tfm Mice via Androgen Receptor-independent Pathway025B3;

Li Zhang1, Da Lei1, Gui-ping Zhu1, Lei Hong2, Sai-zhu Wu2, *   

  1. 1Department of Cardiology, First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510080, China 
    2Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
  • Received:2012-10-15 Online:2013-06-26 Published:2013-06-26
  • Contact: 86-20-61642151,E-mail:wusaizu@126.com025B3;SupportedbyNationalBasicResearchProgramofChina(973Program,2007CB507404).
  • Supported by:

    Supported by National Basic Research Program of China (973 Program, 2007CB507404).

Abstract:

Male littermates and testicular feminized (Tfm) mice were randomly separated into 4 experimental groups littermate controls (n=8), Tfm mice (n=7), testosterone-treated Tfm mice (n=8), and Tfm mice treated with testosterone in combination with the aromatase inhibitor anastrazole (n=7). Cardiomyocytes were isolated from mouse left ventricles, the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and the amount of malondialdehyde (MDA) were measured using colorimetry method, and expression of p16INK4α and retinoblastoma (Rb) proteins were detected by Western blotting.

The SOD and GSH-Px enzyme activities of cardiomyocytes were decreased, and the MDA levels and the expression of p16INK4α and Rb proteins were increased in Tfm mice compared with control mice. An increase was observed in the activities of SOD and GSH-Px enzyme as well as a decrease in MDA levels and the expression of p16INK4α and Rb proteins in the testosterone-treated Tfm mice. After co-treatment with anastrazole in Tfm mice, these improvement were partly inhibited.

Physiological testosterone replacement can delay cardiomyocyte aging in Tfm mice, an effect that is independent of the AR pathway and in part conversion to estradiol.

Key words: aging, cardiomyocyte, testosterone, androgen receptor

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