Chinese Medical Sciences Journal ›› 2011, Vol. 26 ›› Issue (2): 91-97.doi: 10.1016/S1001-9294(11)60026-6

• Original Article • 上一篇    下一篇

Alpha-GalCer Administration after Allogeneic Bone Marrow Transplantation Improves Immune Reconstitution in Mice

Jing-hua Liu1, 2, Li-ping Dou1, Li-xin Wang1, Li-li Wang1,Fan Zhou2, and Li Yu1*   

  1. 1Department of Hematology, General Hospital of the Chinese People’s Liberation Army, Beijing 100853, China
    2Department of Hematology, Shenyang Military General Hospital, Shenyang 110016, China
  • 收稿日期:2011-06-24 修回日期:2011-06-24 出版日期:2011-06-24 发布日期:2011-06-24

Alpha-GalCer Administration after Allogeneic Bone Marrow Transplantation Improves Immune Reconstitution in Mice

Jing-hua Liu1, 2, Li-ping Dou1, Li-xin Wang1, Li-li Wang1,Fan Zhou2, and Li Yu1*   

  1. 1Department of Hematology, General Hospital of the Chinese People’s Liberation Army, Beijing 100853, China
    2Department of Hematology, Shenyang Military General Hospital, Shenyang 110016, China
  • Received:2011-06-24 Revised:2011-06-24 Online:2011-06-24 Published:2011-06-24

摘要: Objective To explore the effect of α- galactosyleramide( α-GalCer ) on immune reconstitution under acute graft-versus-host disease (aGVHD). Methods BALB/c mice were transplanted wit hallogeneic C57BL/6 bone marrow cells and splenocytes (both 1×107)after receiving lethal total-body irradiation. α-GalCer (100 ug/kg) or vehicle (dimethylsulfoxide) was administered intraperitoneally immediately after transplantation. The effects of α-GalCer on immune reconstitution,proliferation of T cells and B cells, hematopoiesis,and thymic microenvironment were assessed. Results The α-GalCer group exhibited higher percentages of CD3+,CD4+, CD8+, B220+, CD40+, and CD86+cells compared with the vehicle group . The number of colony forming unit per 1000 CD34+ cells in the α-GalCer group was higher than in the vehicle group ( P=0.0012).In vitro proliferation assays showed that the α-GalCer group had higher percentages of CD3+, CD4+, CD8+,and B220+ cells compared with the vehicle group. As for the results of in vivo proliferation assays, the numbers of CD3+, CD4+, CD8+, and B220+cells were higher in the α-GalCer group than in the normal group ,especially the number of B220+ cells ( P=0.007).Significant difference was not found in thymocyte count between the α-GalCer group and the vehicle group, nor in the percentages of CD3+, CD4+, and CD8+ cells.Conclusion Administration of α-GalCer after allogeneic bone marrow transplantation may promote immune reconstitution in the presence of aGVHD.

关键词: immune reconstitution, α-galactosyleramide, bone marrow transplantation

Abstract: Objective To explore the effect of α- galactosyleramide( α-GalCer ) on immune reconstitution under acute graft-versus-host disease (aGVHD). Methods BALB/c mice were transplanted wit hallogeneic C57BL/6 bone marrow cells and splenocytes (both 1×107)after receiving lethal total-body irradiation. α-GalCer (100 ug/kg) or vehicle (dimethylsulfoxide) was administered intraperitoneally immediately after transplantation. The effects of α-GalCer on immune reconstitution,proliferation of T cells and B cells, hematopoiesis,and thymic microenvironment were assessed. Results The α-GalCer group exhibited higher percentages of CD3+,CD4+, CD8+, B220+, CD40+, and CD86+cells compared with the vehicle group . The number of colony forming unit per 1000 CD34+ cells in the α-GalCer group was higher than in the vehicle group ( P=0.0012).In vitro proliferation assays showed that the α-GalCer group had higher percentages of CD3+, CD4+, CD8+,and B220+ cells compared with the vehicle group. As for the results of in vivo proliferation assays, the numbers of CD3+, CD4+, CD8+, and B220+cells were higher in the α-GalCer group than in the normal group ,especially the number of B220+ cells ( P=0.007).Significant difference was not found in thymocyte count between the α-GalCer group and the vehicle group, nor in the percentages of CD3+, CD4+, and CD8+ cells.Conclusion Administration of α-GalCer after allogeneic bone marrow transplantation may promote immune reconstitution in the presence of aGVHD.

Key words: immune reconstitution, α-galactosyleramide, bone marrow transplantation

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