Chinese Medical Sciences Journal ›› 2022, Vol. 37 ›› Issue (2): 142-150.doi: 10.24920/003991

• 论著 • 上一篇    下一篇

硒蛋白脱碘酶的生物学功能及其在骨性关节炎患者中的表达

任晓梅1,张立2,辛宝1,刘启玲3,钱文文1,张荣强3,*()   

  1. 1陕西中医药大学公共卫生学院营养与食品卫生学教研室,陕西咸阳 712046
    2青岛大学医学院附属烟台毓璜顶医院临床营养科,山东烟台 264000
    3陕西中医药大学公共卫生学院流行病与卫生统计学教研室,陕西咸阳 712046
  • 收稿日期:2021-09-06 接受日期:2022-04-08 出版日期:2022-06-30 发布日期:2022-07-07
  • 通讯作者: 张荣强 E-mail:zhangrqxianyang@163.com

Biological Functions of Selenoprotein Iodothyronine Deiodinase and its Expression in Osteoarthritis

Xiaomei Ren1,Li Zhang2,Bao Xin1,Qiling Liu3,Wenwen Qian1,Rongqiang Zhang3,*()   

  1. 1Department of Nutrition and Food Hygiene, School of Public Health, Shaanxi University of Chinese Medicine, Xianyang, Shaanxi 712046, China
    2Department of Clinical Nutrition, The Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Yantai, Shandong 264000, China
    3Department of Epidemiology and Medical Statistics, School of Public Health, Shaanxi University of Chinese Medicine, Xianyang, Shaanxi 712046, China
  • Received:2021-09-06 Accepted:2022-04-08 Published:2022-06-30 Online:2022-07-07
  • Contact: Rongqiang Zhang E-mail:zhangrqxianyang@163.com

摘要:

目的 骨关节炎(osteoarthritis,OA)是一种常见的关节疾病,老年人尤其高发。碘甲状腺原氨酸脱碘酶(iodothyronine deiodinases,DIOs)作为一种重要的硒蛋白,在骨和关节疾病中发挥着关键作用。本研究旨在利用生物信息学方法探讨DIOs在OA发病机制中的作用。
方法 通过对NCBI的GEO数据库中与OA相关的芯片数据进行数据挖掘,利用GenCLip 3.0,DAVID,STRING,Cytoscape和Network Analyst等工具分析DIOs的生物学功能,并用Review Manager5.3对数据进行Meta分析,研究DIOs在正常人和骨性关节炎患者中的表达。
结果 聚类分析显示:DIOs的功能与甲状腺激素受体和碘甲状腺原氨酸有关。GO分析显示:DIOs主要参与乙醇代谢和含酚化合物代谢等生物过程,还与外源生物的细胞色素P450代谢和甲状腺激素信号传导有关。SULT1A1是PPI网络的核心节点。miRNAs和甲状腺激素与DIO1和DIO2存在交互作用。Meta分析显示:DIO3的表达在OA患者中显著上调(P <0.05)。
结论 DIOs的主要生物学功能与甲状腺激素的调节密切相关,而DIO3的上调表达可能在OA的发生中发挥关键作用。

关键词: 硒蛋白, 碘甲状腺原氨酸脱碘酶, 骨关节炎

Abstract:

Objective Iodothyronine deiodinases (DIOs) are important selenoproteins that play a key role in the bone and joint diseases. Osteoarthritis (OA) is the most prevalent joint disease especially in elders. This bioinformatic analysis was performed to explore the role of DIOs in OA pathogenesis.
Methods The biological functions of selenoprotein DIOs were analyzed by bioinformatic techniques, including GenCLip 3.0, Database for Annotation, Visualization and Integrated Discovery (DAVID), STRING, Cytoscape, and Network Analyst. The expression of DIOs in the healthy individuals and OA patients was determined by mining OA-related microarray data in the gene expression omnibus (GEO) database of National Center for Biotechnology Information and performing a Meta-analysis of the data with Review Manager 5.3.
Results Cluster analysis revealed that the function of the DIOs was associated with thyroid hormone receptor and iodothyronine; GO analysis showed that DIOs were mainly involved in biological processes, such as ethanol metabolism and phenol-containing compound metabolism and primarily involved in the cytochrome P450 metabolism of exogenous organisms and thyroid hormone signaling; SULT1A1 was the core node of the PPI network; miRNAs and thyroid hormones had some iterations with DIO1and DIO2; Meta-analysis showed that DIO3 expression was significantly up-regulated in OA patients (SMD = 0.31, 95%CI: 0.03, 0.59, P = 0.03).
Conclusions The main biological functions of DIOs were closely associated with the regulation of thyroid hormone. And the up-regulated expression of DIO3 may have crucial impact on the occurrence of OA.

Key words: selenoprotein, iodothyronine deiodinase, osteoarthritis

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