Chinese Medical Sciences Journal ›› 2018, Vol. 33 ›› Issue (3): 167-173.doi: 10.24920/03476

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  1. 1 中国医学科学院 基础医学研究所 北京协和医学院 基础学院 医学分子生物学国家重点实验室,北京 100005
    2 北京工业大学附属中学, 北京 100022
  • 出版日期:2018-09-30 发布日期:2018-09-28
  • 通讯作者: 许琪

Comparison of β-Amyloid Plaque Labeling Methods: Antibody Staining, Gallyas Silver Staining, and Thioflavin-S Staining

Shi Xinze1,Wei Xuan2,Sha Longze1,Xu Qi1,*()   

  1. 1 State Key Laboratory of Medical Molecular Biology and Neuroscience Center, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences & School of Basic Medicine Peking Union Medical College, Beijing 100005, China;
    2 High School Attached to Beijing University of Technology,Beijing 100022, China
  • Published:2018-09-30 Online:2018-09-28
  • Contact: Xu Qi


目的 探究阿尔茨海默病小鼠模型的淀粉样沉淀的形成过程。比较抗体染色,Gallyas银染和硫磺素S染色3种染色技术检测淀粉样沉淀的效果。方法 检测不同月龄的APPswe/PS1dE9转基因小鼠(APP/PS1)大脑淀粉样沉积的时空变化。对小鼠同一脑区的临近切片行抗体染色,Gallyas银染和硫磺素S染色,并对比其染色效果。结果 随着模型鼠年龄的增大,淀粉样沉积首先出现在的大脑皮层,随后扩散至整个大脑。与Gallyas银染和硫磺素S染色比较,6E10抗体染色能检测到更多的淀粉样斑块沉积(P<0.05)。Gallyas银染和硫磺素S染色的检测结果之间没有显著的统计学意义(P = 0.0033)。结论 APP/PS1转基因小鼠模型能够模拟阿尔茨海默病患者脑内的淀粉样沉积过程。抗体染色得到的结果相较于其它两种检测方法更为可靠。

关键词: 淀粉样沉淀, 阿尔茨海默病, 抗体染色, Gallyas银染, 硫磺素S染色


Objective To evaluate senile plaque formation and compare the sensitivity of three different β-amyloid (Aβ) labeling methods (antibody staining, Gallyas silver staining, and thioflavin-S staining) to detect Aβ deposition.Methods APPswe/PSEN1dE9 transgenic mice (APP/PS1) of different ages were used to examine spatiotemporal changes in Aβ plaque deposition. Antibody staining, Gallyas silver staining, and thioflavin-S staining were used to detect Aβ plaque deposition in the same brain region of adjacent slices from model mice, and the results were compared.Results With aging, Aβ plaques first appeared in the cortex and then the deposition increased throughout the whole brain. Significantly greater plaque deposition was detected by 6E10 antibody than that analyzed with Gallyas silver staining or thioflavin-S staining (P<0.05). Plaque deposition did not show significant difference between the APP/PS1 mice brains assayed with Gallyas silver staining and ones with thioflavin-S staining (P=0.0033).Conclusions The APP/PS1 mouse model of Alzheimer’s disease could mimick the progress of Aβ plaques occurred in patients with Alzheimer’s disease. Antibody detection of Aβ deposition may be more sensitive than chemical staining methods.

Key words: β-amyloid plaques, Alzheimer’s disease, antibody staining, Gallyas silver, thioflavin-S

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