Chinese Medical Sciences Journal ›› 2023, Vol. 38 ›› Issue (3): 206-217.doi: 10.24920/004198
收稿日期:
2022-12-06
接受日期:
2023-06-05
出版日期:
2023-09-30
发布日期:
2023-07-04
通讯作者:
* 沈维增, E-mail:Chao Huang,Hao-Sheng Liu,Bing-Jun Liang,Sheng-Rong Liao,Wei-Zeng Shen*()
Received:
2022-12-06
Accepted:
2023-06-05
Published:
2023-09-30
Online:
2023-07-04
Contact:
* E-mail:摘要:
背景与目的 探讨中药复方防感汤对SARS-CoV-2刺突蛋白诱导的肺、肠损伤的体外和体内保护作用及其机制。
方法 用重组SARS-CoV-2刺突蛋白刺激BALB/c雌性小鼠和3株经防感汤预处理的细胞系。检测各组组织苏木精-伊红(HE)染色、病理评分、细胞通透性和活力以及肺、结肠ACE2表达。酶联免疫吸附测定法(ELISA)检测小鼠血清和细胞上清中炎症因子水平。蛋白质印迹法(Western blotting)检测NF-κB p65、p-NF-κB p65、p- IκB α、p-Smad2/3、TGF-β1、Caspase3、Bcl-2的表达。
结果 从病理评分以及细胞通透性和活力来看,中草药在体内和体外均对刺突蛋白诱发的肺和结肠损伤均有保护作用(P<0.05)。中草药上调肺和结肠中被刺突蛋白降低的ACE2表达,显著改善刺突蛋白引起的炎症标志物的分泌紊乱,同时调节TGF-β/Smads和NF-κB通路的活性。
结论 中药复方防感汤对SARS-CoV-2刺突蛋白刺激的肺和肠组织损伤具有一定的保护作用,其机制基于对可能具有组织类型特异的NF-κB和TGF-β1/Smad通路的调控作用。
Chao Huang, Hao-Sheng Liu, Bing-Jun Liang, Sheng-Rong Liao, Wei-Zeng Shen. A Chinese Herb Prescription “Fang-gan Decoction” Protects Against Damage to Lung and Colon Epithelial Cells Caused by the SARS-CoV-2 Spike Protein by Regulating the TGF-β/Smad2/3 and NF-κB Pathways[J].Chinese Medical Sciences Journal, 2023, 38(3): 206-217.
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Markers | Time | Normal group | Model Gorup | NS group | TCM group | P value |
---|---|---|---|---|---|---|
IL-6 | 3d | 644.61 | 717.36* | 696.89* | 702.85* | 0.009 |
5d | 268.02 | 342.09* | 344.88* | 298.89#? | <0.001 | |
IL-10 | 3d | 25.14 | 82.12* | 74.86* | 44.7# | 0.009 |
5d | 87.61 | 145.79* | 123.84* | 121.52 | 0.019 | |
IL-13 | 3d | 352.69 | 344.69 | 362.46 | 351.09 | 0.998 |
5d | 91.9 | 102.87 | 92.42 | 100.23 | 0.931 | |
TNF-α | 3d | 566.19 | 853.87 | 840.04 | 633.38 | 0.141 |
5d | 575.77 | 1042.36* | 1236.73*# | 864.43*#? | <0.001 | |
CRP | 3d | 18175.8 | 21129.27* | 21550.72* | 19786.15 | 0.042 |
5d | 5933.09 | 7413.08* | 6567.13# | 6093.33# | 0.001 |
"
Markers | Control group | S model group | S model+10% TCM group | S model+25% TCM group | S model+50% TCM group | P value |
---|---|---|---|---|---|---|
IL-6 (ng/L) | 20.24 | 33.01* | 29.81*# | 27.95*# | 20.01# | <0.001 |
IL-10 (ng/L) | 428.32 | 435.40 | 435.54 | 435.38 | 418.36 | 0.762 |
IL-13 (ng/L) | 41.33 | 27.17* | 30.75*# | 33.67*# | 35.04*# | <0.001 |
TNF-α (ng/L) | 212.14 | 309.29* | 277.51* | 263.84 | 222.83 | <0.001 |
NF-κB (ng/L) | 215.69 | 187.85 | 183.57 | 206.98 | 213.29 | 0.154 |
TGF-β1 (ng/L) | 285.49 | 156.97* | 185.03*# | 196.94*# | 272.26# | <0.001 |
"
Cells | Markers | Control group | S model group | S model+10% TCM group | S model+25% TCM group | S model+50% TCM group | P value |
---|---|---|---|---|---|---|---|
HsIMECs | IL-6 (ng/L) | 22.40 | 30.49* | 27.62* | 25.39# | 22.11# | <0.001 |
IL-10 (ng/L) | 348.14 | 307.22* | 318.12* | 327.84*# | 339.72# | 0.001 | |
IL-13 (ng/L) | 30.47 | 30.59 | 28.49 | 31.09 | 29.16 | 0.757 | |
TNF-α (ng/L) | 121.90 | 273.29* | 271.30* | 234.02* | 202.70*# | <0.001 | |
NF-κB (ng/L) | 178.52 | 177.08 | 175.88 | 178.74 | 180.86 | 0.859 | |
TGF-β1 (ng/L) | 149.71 | 146.78 | 143.00 | 141.65 | 145.37 | 0.986 | |
HCoEpiCs | IL-6 (ng/L) | 24.13 | 28.28* | 25.26# | 23.78# | 21.95# | 0.001 |
IL-10 (ng/L) | 372.10 | 384.02 | 378.86 | 388.56 | 388.92 | 0.910 | |
IL-13 (ng/L) | 23.69 | 15.99* | 20.01 | 22.77# | 21.53# | 0.021 | |
TNF-α (ng/L) | 168.63 | 286.16* | 281.62* | 230.50*# | 221.83*# | <0.001 | |
NF-κB (ng/L) | 133.02 | 131.88 | 134.09 | 126.03 | 134.28 | 0.201 | |
TGF-β1 (ng/L) | 177.31 | 173.80 | 176.35 | 177.77 | 171.37 | 0.452 |
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