Chinese Medical Sciences Journal ›› 2013, Vol. 28 ›› Issue (1): 50-54.doi: 10.1016/S1001-9294(13)60019-X

• REVIEW • Previous Articles     Next Articles

Generation of Transgene-free Induced Pluripotent Stem Cells with Non-viral Methods

Tao Wang1, 2, 3, Hua-shan Zhao4, 5, Qiu-ling Zhang1, Chang-lin Xu2, 3, and Chang-bai Liu1*   

  1. 1Institute of Molecular Biology, 2Third Clinical Medical School, 3First Clinical Medical School, 4Department of Neurosurgery, Gezhouba Central Hospital, 5Yichang City Central Hospital, China Three Georges University, Yichang 443002, China
  • Received:2012-06-18 Published:2013-03-26 Online:2013-03-26
  • About author:Corresponding author Tel: 86-717-6397179, E-mail: cbliu@ctgu.

Induced pluripotent stem (iPS) cells were originally generated from mouse fibroblasts by enforced expression of Yamanaka factors (Oct3/4, Sox2, Klf4, and c-Myc). The technique was quickly reproduced with human fibroblasts or mesenchymal stem cells. Although having been showed therapeutic potential in animal models of sickle cell anemia and Parkinson’s disease, iPS cells generated by viral methods do not suit all the clinical applications. Various non-viral methods have appeared in recent years for application of iPS cells in cell transplantation therapy. These methods mainly include DNA vector-based approaches, transfection of mRNA, and transduction of reprogramming proteins. This review summarized these non-viral methods and compare the ad-vantages, disadvantages, efficiency, and safety of these methods.

Key words: induced pluripotent stem cells, non-viral methods, transcription factor, regenerative medicine

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