Chinese Medical Sciences Journal ›› 2010, Vol. 25 ›› Issue (1): 20-26.doi: 10.1016/S1001-9294(10)60015-6

• Original Article • Previous Articles     Next Articles

Expression of FLICE-inhibitory Protein in Synovial Tissue and Its Association with Synovial Inflammation in Juvenile Idiopathic Arthritis

Feng-xia Wu1; Li-jun Wu2; Xiong-yan Luo1; Ming-hui Yang1; Zhong Tang1; Chuan-mei Xie1; Jing-guo Zhou1; Jian-long Guan3; and Guo-hua Yuan1   

  1. 1Institute of Rheumatology and Immunology; Affiliated Hospital of North Sichuan Medical College; Nanchong; Sichuan 637000; China 
    2Department of Rheumatology; People’s Hosptial of Xinjiang Uygur Autonomus Region; Urumchi 830001; China 
    3Department of Rheumatology and Immunology; Changhai Hospital; Second Military Medical University; Shanghai 200433; China
  • Online:2010-03-10 Published:2010-03-10

Abstract: Objective To examine the expression of FLICE-inhibitory protein (FLIP) in juvenile idiopathic arthritis (JIA) and analyze its correlation with synovial inflammation. Methods The expression of FLIP was assessed in 11 JIA and 3 normal synovial tissue samples by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. The cell types expressing FLIP were further characterized,and the correlation of FLIP expression with the degree of synovial inflammation,as well as the activity of caspase 8 was then analyzed. Results RT-PCR revealed the expression of FLIP mRNA in all 11 JIA samples,but not in 3 normal synovial tissues. In JIA,FLIP expression could be found in both the lining and sublining layers,mainly in the macrophage-like cells. Moreover,the expression of FLIP in JIA synovial tissues was positively correlated with the degree of synovial inflammation (r=0.563,P<0.05). Conclusion The expression of antiapoptotic FLIP in JIA synovial tissue and its correlation to accumulation of inflammatory cells in synovial tissue suggests that FLIP potentially extends the lifespan of synovial cells and thus contributes to the progression of joint destruction.

Key words: juvenile idiopathic arthritis, FLICE-inhibitory protein, caspase 8

Funding: Supported by grants from the Chinese Ministry of Health (2008BAI59B01);; the Department of Health of Sichuan province (020035)

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