Chinese Medical Sciences Journal ›› 2011, Vol. 26 ›› Issue (1): 43-48.doi: 10.1016/S1001-9294(11)60018-7

• Original Article • Previous Articles     Next Articles

Influence of Shenqing Recipe on Morphology and Quantity of Colonic Interstitial Cells of Cajal in Trinitrobenzene Sulfonic Acid Induced Rat Colitis

Yan-cheng Dai, Zhi-peng Tang, Zhen-nan Wang, Ya-li Zhang, and Xin-ying He   

  1. Department of Gastroenterology, Longhua Hospital ; Institute of Digestive Disease , Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2010-07-12 Revised:2011-04-18 Online:2011-04-18 Published:2011-04-18
  • Contact: Zhi-peng Tang
  • About author:Tel: 86-21-64385700-3923, Fax: 86-21-64392310,

Abstract: Objective To observe the influence of Shenqing Recipe (SQR), a kind of Traditional Chinese Medicine, on the morphology and quantity of colonic interstitial cells of Cajal (ICC) in trinitrobenzene sulfonic acid (TNBS)-induced rat colitis, and to investigate the possible mechanism of SQR in regulating intestinal dynamics. Methods Sixty rats were randomly divided into normal control, model Ⅰ, model Ⅱ, mesalazine, and high-dose, and low-dose SQR groups with 10 rats in each group. TNBS (10 mg) dissolved in 50% ethanol was instilled into the lumen of the rat colon of the latter five groups to induce colitis. On the 4th day after administration of TNBS, each treatment group was administered one of the following formulations by enteroclysis gavage once a day for 7 days: 600 mg•kg-1•d-1 mesalazine, 2.4 g•kg-1•d-1 SQR, and 1.2 g•kg-1•d-1 SQR. Model Ⅱ rats received normal saline solution. After 7 days colonic samples were collected. While the colonic samples of model Ⅰ group were collected on the 3rd day after TNBS administered. Ultrastructure of ICC in the damaged colonic tissues was observed with transmission electron microscope. Expression of c-kit protein in colonic tissue was determined by immunohistochemical staining and Western blot. Results The ultrastructure of colonic ICC in the rat model of TNBS-induced colitis showed a severe injury, and administration of SQR or mesalazine reduced the severity of injury. Similarly, the expression of c-kit protein of TNBS-induced colitis rat model was significantly decreased compared with the normal control group (P<0.05). Treatment with SQR or mesalazine significantly increased the expression of c-kit protein compared with the administration of control formulations (P<0.05), especially the high-dose SQR group. Conclusion SQR could alleviate and repair the injured ICC, and improve its quantity, which might be involved in regulating intestinal motility.

Key words: Shenqing Recipe, trinitrobenzene sulfonic acid, colitis, c-kit, interstitial


The Shanghai Committee of Science and Technology Foundation for Research Discipline Project (06411941) and the Shanghai Leading Academic Discipline Project (J50305).

Copyright © 2021 Chinese Academy of Medical Sciences.  京公安备110402430088  京ICP备06002729号-1  Powered by Magtech.

Supervised by National Health Commission of the People's Republic of China

9 Dongdan Santiao, Dongcheng district, Beijing, 100730 China

Tel: 86-10-65105897  Fax:86-10-65133074 


Copyright © 2018 Chinese Academy of Medical Sciences

All right reserved.

京公安备110402430088  京ICP备06002729号-1