Chinese Medical Sciences Journal ›› 2017, Vol. 32 ›› Issue (2): 107-112.doi: 10.24920/J1001-9294.2017.018
• Original Article • Previous Articles Next Articles
Prevalence of Carbapenem-Resistant Klebsiella Pneumoniae (CRKP) and the Distribution of Class 1 Integron in Their Strains Isolated from a Hospital in Central China
Ou Qin1, Li Wenfang2, Li Bei1, *(
), Yu Chunfang1
- 1Department of Medical Microbiology, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, China
2Laboratory of Infectious Diseases, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, China
-
Received:2014-04-17Published:2017-06-30Online:2017-06-10 -
Contact:Li Bei E-mail:151449176@qq.com
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Ou Qin, Li Wenfang, Li Bei, Yu Chunfang. Prevalence of Carbapenem-Resistant Klebsiella Pneumoniae (CRKP) and the Distribution of Class 1 Integron in Their Strains Isolated from a Hospital in Central China[J].Chinese Medical Sciences Journal, 2017, 32(2): 107-112.
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Table 1
Distribution of isolated CRKP strains in departments of a hospital in Shiyan, central China（n=117）"
| Department | ICU | Burn | Geriatrics | Emergency | Oncology | General medicine | General surgery | Brain Surgery |
|---|---|---|---|---|---|---|---|---|
| Strains（n） | 52 | 13 | 11 | 9 | 7 | 5 | 4 | 5 |
| Percentage(％) | 44.4 | 11.1 | 9.4 | 7.7 | 6.0 | 4.3 | 3.4 | 4.3 |
| Department | Orthopaedics | Neurology | Gastroenteroloy | Respiratory | Cardiology | Hematology | Rheumatology | Thoracic |
| Strains（n） | 2 | 1 | 1 | 1 | 1 | 1 | 2 | 2 |
| Percentage(％) | 1.7 | 0.9 | 0.9 | 0.9 | 0.9 | 0.9 | 1.7 | 1.7 |
Table 1
Table 2
Distribution of antibiotics resistance in 117 strains of CRKP isolated from a hospital in Shiyan, central China [n, (%)]"
| Suscepti- bility | Antibiotics | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ampicillin /Sulbactam | Amikacin | Aztreonam | Tigecycline | Colistin | Imipenem | Meropenem | ||||||
| Sensitive | 0 | 9 (7.7) | 3(2.6） | 69(59.0) | 63(53.8) | 1(0.9) | 2(1.7) | |||||
| Medium sensitive | 0 | 3(2.6) | 2(1.7) | 33(28.2) | 12(10.3) | 2(1.7) | 3(2.6) | |||||
| Resistant | 117(100) | 105(89.7) | 112(95.7) | 15(12.8) | 42(35.9) | 114(97.4) | 112(95.7) | |||||
| Suscepti- bility | Antibiotics | |||||||||||
| Tobramycin | Ceftazidme | Gentamicin | Cefotaxime | Cefepime | Piperacillin | |||||||
| Sensitive | 7(6.0) | 0 | 8(6.8) | 0 | 1(0.9) | 0 | ||||||
| Medium sensitive | 6(5.1) | 3(2.6) | 3(2.6) | 0 | 3(2.6) | 2(1.7) | ||||||
| Resistant | 104(88.9) | 114(97.4) | 106(90.6) | 117(100) | 113(96.5) | 115(98.3) | ||||||
Table 2
Table 3
Association between class 1 integron and antimicrobial resistant phenotypes in 117 strains of CRKP [n, (%)]"
| Class 1 integron | Strains resistant to antibiotics | |||||||
|---|---|---|---|---|---|---|---|---|
| Ampicillin/ Sulbactam (n=117) | Amikacin (n=105) | Aztreonam (n=112) | Tigecycline (n=15) | Colistin (n=42) | Imipenem (n=114) | Meropenem (n=112) | ||
| Negative (n=26) | 26 | 18 | 24 | 5 | 11 | 25 | 25 | |
| (100) | (69.2) | (92.3) | (19.2) | (42.3) | (96.2) | (96.2) | ||
| Positive (n=91) | 91 | 87 | 88 | 10 | 31 | 89 | 87 | |
| (100) | (95.6) | (96.7) | (10.9) | (34.1) | (97.8) | (95.6) | ||
| P-value | 1 | 0.001 | 0.328 | 0.268* | 0.440† | 0.220 | 0.903 | |
| Class 1 integron | Strains resistant to antibiotics | |||||||
| Piperacillin (n=115) | Cefotaxime (n=117) | Cefepime (n=113) | Tobramycin (n=103) | Ceftazidime (n=114) | Gentamicin (n=106) | |||
| Negative (n=26) | 25 | 26 | 25 | 17 | 25 | 18 | ||
| (100) | (69.2) | (96.2) | (65.3) | (96.2) | (69.2) | |||
| positive (n=91) | 90 | 91 | 88 | 86 | 89 | 88 | ||
| (98.9) | (95.6) | (96.7) | (94.5) | (97.8) | (96.7) | |||
| P-value | 1 | 1 | 1 | 0.000 | 1 | 0.000 | ||
Table 3
| 1. | Jia Li, Fei Liu, Qi Wang, et al.Genomic and transcriptomic analysis of NDM-1 Klebsiella pneumoniae in spaceflight reveal mechanisms underlying environmental adaptability. Sci Rep 2014; 4: 6216. doi:10.1038/srep06216. |
| 2. | Camargo JF, Simkins J, Beduschi T, et al.Successful treatment of Carbapenemase-producing pandrug-resistant Klebsiella pneumoniae bacteremia. Agents Chemother 2015; 59: 5903-8. doi:10.1128/AAC.00655-15. |
| 3. | Messina JA, Cober E, Richter SS, et al.Hospital readmissions in patients with carbapenem-resistant Klebsiella pneumoniae. Infect Control HospEpidemiol 2016; 37: 281-8. doi:10.1017/ice.2015.298. |
| 4. | Saidel-Odes L, Borer A.Limiting and controlling carbapenem-resistant Klebsiella pneumoniae. Infect Drug Resist 2013; 7: 9-14. doi:10.2147/IDR.S44358. |
| 5. | Liu LT, Wan LH, Song XH, et al.Relevance of class 1 integrons and extended-spectrum β-lactamases in drug- resistant Escherichia coli. Mol Med Rep 2013; 8:1251-5. doi:10.3892/mmr.2013.1626. |
| 6. | Pan YJ, Lin TL, Lin YT, et al.Identification of capsular types in carbapenem-resistant Klebsiellapneumoniae strains by wzc sequencing and implications for capsule depolymerase treatment. Antimicrob Agents Chemother 2015; 59:1038-47. doi:10.1128/AAC.03560-14. |
| 7. | Diago-Navarro E, Chen L, Passet V, et al.Carbapenem- resistant Klebsiella pneumoniae exhibit variability in capsular polysaccharide and capsule associated virulence traits. J Infect Dis 2014; 210: 803-13. doi:10.1093/infdis/jiu157. |
| 8. | Odumosu BT, Adeniyi BA, Chandra R.Analysis of integrons and associated gene cassettes in clinical isolates of multidrug resistant Pseudomonas aeruginosa from Southwest Nigeria. Ann Clin Microbiol Antimicrob 2013; 12: 29. doi:10.1186/1476-0711-12-29. |
| 9. | Rocha LK, dos Santos Neto RL, da Costa Guimarães AC, et al. Plasmid-mediated qnrA1 in Klebsiella pneumoniae ST147 in Recife, Brazil. Int J Infect Dis 2014; 26:49-50. doi:10.1016/j.ijid.2014.04.026. |
| 10. | Candan ED, Aksöz N.Klebsiella pneumoniae: characteristics of carbapenem resistance and virulence factors. ActaBiochim Pol 2015; 62:867-74. doi:10.18388/abp.2015_ 1148. |
| 11. | Shields RK, Clancy CJ, Hao B, et al.Effects of Klebsiella pneumoniae carbapenemase subtypes, extended-spectrum β-lactamases, and porin mutations on the in vitro activity of ceftazidime-avibactam against carbapenem-resistant K. pneumoniae. Antimicrob Agents Chemother 2015; 59: 5793-7. doi:10.1128/AAC.00548-15. |
| 12. | Guo W, Shan K, Xu B, et al.Determining the resistance of carbapenem-resistant Klebsiella pneumoniae to common disinfectants and elucidating the underlying resistance mechanisms. Pathog Glob Health 2015; 109: 184-92. doi:10.1179/2047773215Y.0000000022. |
| 13. | Agodi A, Barchitta M, Quattrocchi A, et al.Antibiotic trends of Klebsiella pneumoniae and Acinetobacter baumannii resistance indicators in an intensive care unit of Southern Italy, 2008-2013. Antimicrob Resist Infect Control 2015; 4:43. doi:10.1186/s13756-015-0087-y. |
| 14. | Hosan Kim, Inder Makin, Jeff Skiba, et al.Antibacterial efficacy testing of a bioelectric wound dressing against clinical wound pathogens. Open Microbiol J 2014; 8: 15-21. doi:10.2174/1874285801408010015. |
| 15. | Mouloudi E, Massa E, Piperidou M, et al.Tigecycline for treatment of carbapenem-resistant Klebsiella pneumonia infections after liver transplantation in the intensive care unit: a 3-year study. Transplant Proc 2014; 46:3219-21. doi:10.1016/j.transproceed.2014.09.160. |
| 16. | Lee JY, Choi MJ, Choi HJ, et al.Preservation of acquired colistinresistance in Gram-negative bacteria. Antimicrob Agents Chemother 2015; 60: 609-12. doi:10.1128/AAC.01574-15. |
| 17. | Chaari A, Mnif B, Chtara K, et al.Efficacy of tigecycline- colistin combination in the treatment of carbapenem- resistant Klebsiella pneumoniae endocarditis. J Glob Antimicrob Resist 2015;3:214-16. doi:10.1016/j.jgar.2015.06.003. |
| 18. | Yang HS, Fang WL, Lin YT.A case of liver abscess caused by tigecycline-nonsusceptible Klebsiella pneumoniae. J Microbiol Immunol Infect 2016;49:621-2. doi:10.1016/j.jmii.2016.04.010. |
| 19. | Zeighami H, Haghi F, Hajiahmadi F.Molecular characterization of integronsin clinical isolates of betalactamase producing Escherichia coli and Klebsiella pneumoniaein Iran. J Chemother 2015; 27:145-51. doi:10.1179/1973947814Y.0000000180. |
| 20. | Huang YW, Hu RM, Lin YT, et al.The contribution of class 1 integron to antimicrobial resistance in Stenotrophomonasmaltophilia. Microb Drug Resist 2015; 21:90-6. doi:10.1089/mdr.2014.0072. |
| 21. | Yuejin Z, Qingli C, Qian W, et al. Structure and function of class 1 integronin clinical isolates of Klebsiella pneumoniae. Yi Chuan 2014; 36: 603-10. Chinese. doi:10.3724/SP.J.1005.2014.0603. |
| 22. | Li B, Hu Y, Wang Q, et al.Structural diversity of class 1 integrons and their associated gene cassettes in Klebsiell- a pneumonia isolates from a hospital in China. PLoS One 2013; 8:e75805. doi:10.1371/journal.pone.0075805. |
| 23. | Liu H, Wang H, Huang M, et al.Analysis of antimicrobial resistance and class 1 integrons among strains from upper respiratory tract of healthy adults. J Thorac Dis 2013; 5:149-55. doi:10.3978/j.issn.2072-1439.2013.03.09. |
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