Dual Isoflurane-induced Preconditioning Improves Neuroprotection in Rat Brain In Vitro and the Role of Extracellular Signal–regulated Protein Kinase

doi:10.1016/S1001-9294(11)60017-5

Abstract

Abstract: Objective To test the ability of isoflurane-induced preconditioning against oxygen and glucose deprivation (OGD) injury in vitro. Methods Rat hippocampal slices were exposed to 1 volume percentage (vol%), 2vol% or 3vol% isoflurane respectively for 20 minutes under normoxic conditions (95% O2/5% CO2) once or twice (12 slices in each group) before OGD, with 15-minute washout after each exposure. During OGD experiments, hippocampus slices were bathed with artificial cerebrospinal fluid (ACSF) lacking glucose and perfused with 95% N2 and 5% CO2 for 14 minutes, followed by a 30-minute reperfusion in normal ACSF. The CA1 population spike (PS) was measured and used to quantify the degree of neuronal function recovery after OGD. To assess the role of mitogen-activated protein kinases (MAPKs) in isoflurane preconditioning, U0126, an inhibitor of extracellular signal-regulated protein kinase (ERK1/2), and SB203580, an inhibitor of p38 MAPK, were used before two periods of 3vol% isoflurane exposure. Results The degree of neuronal function recovery of hippocampal slices exposed to 1vol%, 2vol%, or 3vol% isoflurane once was 41.88%±9.23%, 55.05%±11.02%, or 63.18%±10.82% respectively. Moreover, neuronal function recovery of hippocampal slices exposed to 1vol%, 2vol%, or 3vol% isoflurane twice was 53.75%±12.04%, 63.50%±11.06%, or 76.25%±12.25%, respectively. Isoflurane preconditioning increased the neuronal function recovery in a dose-dependent manner. U0126 blocked the preconditioning induced by dual exposure to 3vol% isoflurane (6.13%±1.56%, P<0.01) and ERK1/2 activities. Conclusions Isoflurane is capable of inducing preconditioning in hippocampal slices in vitro in a dose-dependent manner, and dual exposure to isoflurane with a lower concentration is more effective in triggering preconditioning than a single exposure. Isoflurane-induced neuroprotection might be involved with ERK1/2 activities.

Key words: electrophysiology, hippocampal slice, isoflurane, preconditioning, oxygen and glucose deprivation, mitogen-activatedprotein kinase

Funding:

Foundation of Shihezi University of Xinjiang Province (RCZX200688)

Sheng Wang, Su-xiang Guo, Zhi-gang Dai, Xi-wei Dong, Yang Liu, Shan Jiang, and Zhi-ping Wang. Dual Isoflurane-induced Preconditioning Improves Neuroprotection in Rat Brain In Vitro and the Role of Extracellular Signal–regulated Protein Kinase[J].Chinese Medical Sciences Journal, 2011, 26(1): 36-42.

References 28

1.	Xiong L, Zheng Y, Wu M, et al. Neuroprotection via activation of adenosine triphosphate-regulated potassium channels after focal cerebral ischemia in rats. Anesth Analg 2003; 96:233-7.
2.	Engelhard K, Werner C, Reeker W, et al. Desflurane and isoflurane improve neurological outcome after incomplete cerebral ischemia in rats. Br J Anaesth 1999; 83:415-21.
3.	Wise FL, Raizada MK, Sumners C. Oxygen and glucose deprivation-induced neuronal apoptosis is attenuated by halothane and isoflurane. Anesth Analg 2001; 93:1281-7.
4.	Amour J, Brzezinska AK, Weihrauch D. Role of heat shock protein 90 and endothelial nitric oxide synthase during early anesthetic and ischemic preconditioning. Anesthesiology 2009; 110:317-25.
5.	Zhang J, Yang ZJ, Klaus JA, et al. Delayed tolerance with repetitive transient focal ischemic preconditioning in the mouse. Stroke 2008; 39:967-74.
6.	Sugino T, Nozaki K, Takagi Y, et al. Activation of mito-gen-activated protein kinases after transient forebrain ischemia in gerbil hippocampus. J Neurosci 2000; 20: 4506-14.
7.	Kehl F, Payne RS, Roewer N, et al. Sevoflurane-induced preconditioning of rat brain in vitro and the role of KATP channels. Brain Res 2004; 1021:76-81.
8.	Martin DC, Dennison RL, Introna RP, et al. Influence of halothane on the inter-actions of serotonin1A and adenosine A1 receptors with G proteins in rat brain mem- branes. Biochem Pharmacol 1991; 42:1313-6.
9.	Tas PW, Eisemann C, Roewer N. The volatile anesthetic isoflurane suppresses spontaneous calcium oscillations in vitro in rat hippocampal neurons by activation of adenosine A1 receptors. Neurosci Lett 2003; 338:229-32.
10	Zheng S, Zuo Z. Isoflurane preconditioning decreases glutamate receptor overactivation-induced Purkinje neu- ronal injury in rat cerebellar slices. Brain Res 2005; 1054: 143-51.
11	Weber NC, Toma O, Awan S, et al. Effects of nitrous oxide on the rat heart in vivo: another inhalational anesthetic that preconditions the heart? Anesthesiology 2005; 103: 1174-82.
12	Johnson GL, Lapadat R. Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science 2002; 298:1911-2.
13	Li QF, Zhu YS, Jiang H. Isoflurane preconditioning activates HIF-1alpha, iNOS and Erk1/2 and protects against oxygen-glucose deprivation neuronal injury. Brain Res 2008;1245:26-35.
14	Jiang W, Van CJ, Sheerin AH, et al. Involvement of extracellular regulated kinase and p38 kinase in hippocampal seizure tolerance. J Neurosci Res 2005; 81: 581-8.
15	Hirota K, Roth SH. Sevoflurane modulates both GABAA and GABAB receptors in area CA1 of rat hippocampus. Br J Anaesth 1997; 78:60-5.
16	Bairey O, Vanichkin A, Shpilberg O. Arsenic-trioxide- induced apoptosis of chronic lymphocytic leukemia cells. Int J Lab Hematol 2010; 32:77-85.
17	Mottet D, Michel G, Renard P, et al. Role of ERK and calcium in the hypoxia-induced activation of HIF-1. J Cell Physiol 2003; 194:30-44.
18	Bickler PE, Fahlman CS. Expression of signal transduction genes differs after hypoxic or isoflurane preconditioning of rat hippocampal slice cultures. Anesthesiology 2009; 111: 258-66.
19	McMurtrey RJ, Zuo Z. Isoflurane preconditioning and postconditioning in rat hippocampal neurons. Brain Res 2010; 1358:184-90.
20	Tian GF, Baker AJ. Glycolysis prevents anoxia-induced synaptic transmission damage in rat hippocampal slices. J Neurophysiol 2000; 83:1830-9.
21	Zhang HP, Yuan LB, Zhao RN, et al. Isoflurane precondi-tioning induces neuroprotection by attenuating ubiqui-tin-conjugated protein aggregation in a mouse model of transient global cerebral ischemia. Anesth Analg 2010; 111:506-14.
22	Chi OZ, Hunter C, Liu X, et al. The effects of isoflurane pretreatment on cerebral blood flow, capillary permeability, and oxygen consumption in focal cerebral ischemia in rats. Anesth Analg 2010;110:1412-8.
23	Gray JJ, Bickler PE, Fahlman CS, et al. Isoflurane neuro-protection in hypoxic hippocampal slice cultures involves increases in intracellular Ca2+ and mitogen-activated protein kinases. Anesthesiology 2005; 102:606-15.
24	Nicole O, Ali C, Docagne F, et al. Neuroprotection mediated by glial cell line-derived neurotrophic factor: involvement of a reduction of NMDA-induced calcium influx by the mitogenactivated protein kinase pathway. J Neuroscience 2001; 21:3024-33.
25	Zheng S, Zuo Z. Isoflurane preconditioning induces neuroprotection against ischemia via activation of P38 mitogen-activated protein kinases. Mol Pharmacol 2004; 65:1172-80.
26	Bickler PE, Buck L, Feiner JR. Volatile and intravenous anesthetics decrease glutamate release from cortical brain slices during anoxia. Anesthesiology 1995; 83: 1233-40.
27	Patel PM, Drummond JC, Cole DJ, et al. Isoflurane reduces ischemia-induced glutamate release in rats subjected to forebrain ischemia. Anesthesiology 1995; 82:996-1003.
28	Bickler PE, Warner DS, Stratmann G, et al. GABA receptors contribute to isoflurane neuroprotection in organotypic hippocampal cultures. Anesth Analg 2003; 97:564- 71.

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