Chinese Medical Sciences Journal ›› 2014, Vol. 29 ›› Issue (3): 167-173.doi: 10.1016/S1001-9294(14)60063-8

• Orginal Article • Previous Articles     Next Articles

Phosphatase and Tension Homolog Overexpression in Insulin Resistant Diabetic Adipose Tissue

Jing-bo Zeng1, 2, Yun Zhang1, Qi Sun1, Yu-xiu Li1, *   

  1. 1Department of Endocrinology, Key Laboratory of Endocrinology of the Ministry of Health, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China; 2Department of Endocrinology, Fuxing Hospital Affiliated to Capital University of Medical Sciences, Beijing 100038, China
  • Published:2014-09-29 Online:2014-09-29
  • Contact: *Corresponding author Tel: 86-10-69155088, Fax: 86-10-69155088, E-mail:

Objective To investigate the expression of phosphatase and tension homolog (PTEN) in adipose tissue of KKAy diabetic mice, a mouse model of type 2 diabetes. Methods KKAy diabetic mice were fed with high fat diet for 4 weeks. After blood glucose met the criteria of diabetes (over 16.7 mmol/L), mice were randomly divided into 3 groups: a control group (without any treatment), a rosiglitazone group (treated with rosiglitazone 12.5 mg/kg?d once per day), and a metformin group (treated with metformin 3 g/kg?d twice daily). After 4 weeks, we then determined the expression of PTEN and phosphoserine 473-Akt (pS473-Akt) in the epididymal adipose tissue with Western blots. The mice in each group were further divided into the insulin (-) subgroup and insulin (+) subgroup, which were intraperitoneally injected with saline and insulin (5 mU/g body weight), respectively. Results The expression of PTEN was elevated in the epididymal adipose tissue obtained from KKAy diabetic mice compared with that from the C57BL/6J mice (P<0.001). In accordance with the enhanced expression of PTEN, the level of pS473-Akt stimulated by insulin was decreased in the adipose tissue of KKAy mice compared to the C57BL/6J mice (P<0.001). Treatment with the insulin-sensitizing agents, rosiglitazone and metformin did not inhibit the elevated expression of PTEN in adipose tissue of KKAy diabetic mice. Conclusion PTEN may play an important role in the development of insulin resistance in adipose tissue of type 2 diabetes mice model.

Key words: insulin resistance, phosphatase and tension homolog, adipose tissue

Funding: △Supported by National Natural Science Foundation of China (81270878) and National Key Program of Clinical Science of China (WBYZ2011-873)

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