Chinese Medical Sciences Journal ›› 2017, Vol. 32 ›› Issue (2): 92-99.doi: 10.24920/J1001-9294.2017.020
• Original Article • Previous Articles Next Articles
Neuroprotective Effects of Grape Seed Procyanidin Extract on Ischemia-Reperfusion Brain Injury△
Kong Xiangyi1, 2, Guan Jian1, Gong Shun3, 4, Wang Renzhi1, *(
)
- 1Department of Neurosurgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China
2Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Harvard University, Massachusetts 02114, USA
3Department of Neurosurgery, Shanghai Institute of Neurosurgery, PLA Institute of Neurosurgery, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
4Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Massachusetts 02215, USA
-
Received:2017-02-17Published:2017-06-30Online:2017-06-10 -
Contact:Wang Renzhi E-mail:wrzpumch@163.com
Cite this article
Kong Xiangyi, Guan Jian, Gong Shun, Wang Renzhi. Neuroprotective Effects of Grape Seed Procyanidin Extract on Ischemia-Reperfusion Brain Injury△[J].Chinese Medical Sciences Journal, 2017, 32(2): 92-99.
share this article
Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks
Figure 1.
The neurological severity scores (NSS) for MCAONS mice and MCAOG mice at 1 h, 24 h, 3 d and 7 d after MCAO. Significant differences of NSS were observed at 24h, 3d, and 7d between group MCAONS and MCAOG. *P<0.05."
Figure 1.
Figure 2.
Stroke area after ischemia-reperfusion brain injury was measured by brain MRI (T2WI) 24 h after MCAO. A. High signal intensity was detected on T2WI in brain tissue of MCAOG mice, indicating cerebral infarction (arrow). B. Bar graph showing the mean stroke volume in MCAOG was significantly smaller than that of MCAONS mice. *P<0.001."
Figure 2.
Figure 3.
Comparison of NeuN and CD34 expression in the brain tissue of MCAONS and MCAOG group 24 hours after reperfusion by immunohistochemistry. A. Immunohistochemical staining of brain NeuN expression in MCAONS group (20×), and B. in MCAOG group (20×); C. The counting of mature NeuN immunoreactive neurons in MCAONS group was significantly less than that in the MCAOG group,*P<0.05; D. Immunohistochemical staining of brain CD 34 expression in the MCAONS group (20×), and E. in MCAOG group (20×); F. The counting of CD34-immunoreactive cells in the MCAONS group was significantly less than that in MCAOG group, *P<0.05. Black arrows: positive staining."
Figure 3.
Figure 4.
Comparison of Bax and Bcl-2 expression in the brain tissue of mice between MCAONA and MCAOG group. A. Immunostaining of the Bax-positive cells in MCAONS group (20×) and B. in MCAOG group (20×); C. The number of Bax-positive cells was significantly lower in MCAOG compared to MCAONS, *P<0.05; D. Immunostaining of Bcl-2 positive cells in MCAONS group (20×) and E. in MCAOG group (20×); F. The number of Bcl-2 positive cells was significantly higher in MCAOG group compared to MCAONS group, *P<0.05."
Figure 4.
Figure 5.
Comparison of MDA in brain tissues between the groups with pretreatment of GSPE and Normal saline. The MDA levels in brain tissues of MCAOG group 1 hour and 24 hours after reperfusion were significantly lower than those of MCAONS group (*P<0.05 for both). There was no difference between SONS group and SOG group (P>0.05)."
Figure 5.
Figure 6.
Comparison of GSH-Px in brain tissues between the groups pretreated with GSPE and normal saline. The activity of GSH-Px in the brains of MCAONS group was lower than that of MCAOG group.*P< 0.05."
Figure 6.
| 1. | Feigin VL.Stroke epidemiology in the developing world. Lancet 2005; 365: 2160-1. doi:10.1016/S0140-6736 (05)66755-4. |
| 2. | Allen CL, Bayraktutan U.Oxidative stress and its role in the pathogenesis of ischaemic stroke. Int J Stroke 2009; 4: 461-70. doi:10.1111/j.1747-4949.2009.00387.x. |
| 3. | Moskowitz MA, Lo EH, Iadecola C.The science of stroke: mechanisms in search of treatments. Neuron 2010; 67: 181-98. doi:10.1016/j.neuron.2010.07.002. |
| 4. | Chen T, Liu W, Chao X, et al.Neuroprotective effect of osthole against oxygen and glucose deprivation in rat cortical neurons: involvement of mitogen-activated protein kinase pathway. Neuroscience 2011; 183:203-11. doi:10.1016/j.neuroscience.2011.03.038. |
| 5. | Mehlomakulu NN, Prior KJ, Setati ME, et al.Candida pyralidae killer toxin disrupts the cell wall of Brettanomyces bruxellensis in red grape juice. J Appl Microbiol 2017; 122:747-58. doi:10.1111/jam.13383. |
| 6. | Chen Q, Zhang R, Li WM, et al.The protective effect of grape seed procyanidin extract against cadmium-induced renal oxidative damage in mice. Environ Toxicol Pharmacol 2013; 36: 759-68. doi:10.1016/j.etap.2013.07. 006. |
| 7. | Chen J, Li Y, Wang L, et al.Therapeutic Benefit of Intravenous Administration of Bone Marrow Stromal Cells After Cerebral Ischemia in Rats. Stroke 2001; 32: 1005-11. doi:10.1161/01.STR.32.4.1005. |
| 8. | Gauvrit JY, Leclerc X, Pernodet M, et al.Value of MRI in the etiologic diagnosis of cerebral infarction. J Radiol 2005; 86:1080-9. |
| 9. | Pillai DR, Dittmar MS, Baldaranov D, et al.Cerebral ischemia-reperfusion injury in rats-a 3 T MRI study on biphasic blood-brain barrier opening and the dynamics of edema formation. J Cereb Blood Flow Metab 2009; 29:1846-55. doi:10.1038/jcbfm.2009.106. |
| 10. | Liu F, Schafer DP, Mccullough LD.TTC, fluoro-Jade B and NeuN staining confirm evolving phases of infarction induced by middle cerebral artery occlusion. J Neurosci Methods 2009; 179:1-8. doi:10.1016/j.jneumeth.2008. 12.028. |
| 11. | Kong X, Guan J, Ma W, et al.CD34 Over-expression is associated with Gliomas' higher WHO grade. Medicine (Baltimore) 2016; 95:e2830. doi:10.1097/MD.0000000 000002830. |
| 12. | Bizzi I, Ghezzi P, Paudyal P.Health information quality of websites on periodontology. J Clin Periodontol 2017; 44:308-14. doi:10.1111/jcpe.12668. |
| 13. | Hattori K, Lee H, Hurn PD, et al.Cognitive deficits after focal cerebral ischemia in mice. Stroke 2000; 31:1939-44. |
| 14. | Zanolin ME, Girardi P, Degan P, et al.Measurement of a urinary marker (8-hydroxydeoxy-guanosine, 8-OHdG) of DNA oxidative stress in epidemiological surveys: a pilot study. Int J Biol Markers 2015; 30: e341-5. doi:10.5301/ jbm.5000129. |
| 15. | Liu G, Wang T, Wang T, et al.Effects of apoptosis-related proteins caspase-3, Bax and Bcl-2 on cerebral ischemia rats. Biomed Rep 2013; 1:861-7. doi:10.3892/br.2013. 153. |
| 16. | Zhang JQ, Gao BW, Wang J, et al.Critical role of FoxO1 in granulosa cell apoptosis caused by oxidative stress and protective effects of grape seed procyanidin B2. Oxid Med Cell Longev 2016; 2016:6147345. doi:10.1155/2016/ 6147345. |
| 17. | Terra X, Montagut G, Bustos M, et al.Grape-seed procyanidins prevent low-grade inflammation by modulating cytokine expression in rats fed a high-fat diet. J Nutr Biochem 2009; 20:210-8. doi:10.1016/j.jnutbio.2008.02. 005. |
| 18. | Jin H, Liu M, Zhang X, et al.Grape seed procyanidin extract attenuates hypoxic pulmonary hypertension by inhibiting oxidative stress and pulmonary arterial smooth muscle cells proliferation. J Nutr Biochem 2016; 36:81-8. doi:10.1016/j.jnutbio.2016.07.006. |
| 19. | Yamakoshi J, Saito M, Kataoka S, et al.Safety evaluation of proanthocyanidin-rich extract from grape seeds. Food Chem Toxicol 2002; 40:599-607. |
| 20. | Shahjouei S, Cai PY, Ansari S, et al.Middle cerebral artery occlusion model of stroke in rodents: A step-by-step approach. J Vasc Interv Neurol 2016; 8:1-8. |
| 21. | Aleu A, Mellado P, Lichy C, et al.Hemorrhagic complications after off-label thrombolysis for ischemic stroke. Stroke 2007; 38:417-22. doi:10.1161/01.STR.0000254 504.71955.05. |
| 22. | Kowalczuk K, Stryjecka-Zimmer M.The influence of oxidative stress on the level of malondialdehyde (MDA) in different areas of the rabbit brain. Ann Univ Mariae Curie Sklodowska Med 2002; 57:160-4. |
| 23. | Olsvik PA, Kristensen T, Waagbo R, et al.mRNA expression of antioxidant enzymes (SOD, CAT and GSH-Px) and lipid peroxidative stress in liver of Atlantic salmon (Salmo salar) exposed to hyperoxic water during smoltification. Comp Biochem Physiol C Toxicol Pharmacol 2005; 141:314-23. doi:10.1016/j.cbpc.2005.07.009. |
| 24. | Pallares V, Fernandez-Iglesias A, Cedo L, et al.Grape seed procyanidin extract reduces the endotoxic effects induced by lipopolysaccharide in rats. Free Radic Biol Med 2013; 60:107-14. doi:10.1016/j.freeradbiomed.2013.02.007. |
| [1] | Ping Fen, Cao Qin, Lin Hua, Han Shuzhi. Antagonistic Effects of N-acetylcysteine on Mitogen-activated Protein Kinase Pathway Activation, Oxidative Stress and Inflammatory Responses in Rats with PM2.5 Induced Lung Injuries [J]. Chinese Medical Sciences Journal, 2019, 34(4): 270-276. |
| [2] | Pan Yanfang, Jia Xiaotao, Song Erfei, Peng Xiaozhong. Astragaloside IV Protects Against Aβ1-42-induced Oxidative Stress, Neuroinflammation and Cognitive Impairment in Rats [J]. Chinese Medical Sciences Journal, 2018, 33(1): 29-37. |
| [3] | He Ying,Zhang Ying,Wang Mengying,Zhang Meng,Zhang Dan,Zhang Ying,Jiang Zhuocheng,Wu Feng,Chen Jinghong. Gene Expression Profile of Hypertrophic Chondrocytes Treated with H2O2: A Preliminary Investigation [J]. Chinese Medical Sciences Journal, 2018, 33(1): 45-52. |
| [4] | Zhu Huiyuan, Bie Yulong, Wang Jiang, Gao Jing, Yang Bingyue, Wan Haitong. Experimental Study on the Protection of Agrimony Extracts from Different Extracting Methods against Cerebral Ischemia-Reperfusion Injury△ [J]. Chinese Medical Sciences Journal, 2017, 32(4): 239-247. |
| [5] | Fen Ping, Zhen-sheng Li, Feng-rui Zhang, De-xin Li, Shu-zhi Han. Effects of Lianhua Qingwen on Pulmonary Oxidative Lesions Induced by Fine Particulates (PM2.5) in Rats [J]. Chinese Medical Sciences Journal, 2016, 31(4): 233-238. |
| [6] | Chong-jian Li, Run-lin Gao, Yue-jin Yang, Feng-huan Hu, Wei-xian Yang, Shi-jie You, Lai-feng Song, Ying-mao Ruan, Shu-bin Qiao, Ji-lin Chen and Jian-jun Li. Effect of Intracoronary Infusion of Bone Marrow Mononuclear Cells or Peripheral Endothelial Progenitor Cells on Myocardial Ischemia-reperfusion Injury in Mini-swine [J]. Chinese Medical Sciences Journal, 2010, 25(3): 176-181. |
| Viewed | ||||||
|
Full text |
|
|||||
|
Abstract |
|
|||||
|
