Chinese Medical Sciences Journal ›› 2013, Vol. 28 ›› Issue (1): 16-19.doi: 10.1016/S1001-9294(13)60013-9

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Expression of Plasminogen Activator Inhibitor-2 is Negatively Associated with Invasive Potential in Hepatocellular Carcinoma Cells△

Ye Jin1, Li Zhou2, Ke-min Jin3, and Bao-cai Xing3*   

  1. 1 Clinical Research Laboratory, 2Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China; 3Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Hepatobiliary Surgery I, Peking University Cancer Hospital, Beijing 100142, China
  • Received:2012-08-28 Online:2013-03-26 Published:2013-03-26
  • About author:?These authors contributed equally to this work.*Corresponding author Tel: 86-10-88196558, Fax: 86-10-88196567, E-mail: bcxingsubmission@163.com
  • Supported by:

    △Supported by the National High Technology Research and Development Program of China (863 Program, 2006AA02A308), the National Key Technologies Research and Development Program of China (2008ZX10002-016), and the Beijing Municipal Key Project (H030230280410).

Abstract:

To investigate the association between plasminogen activator inhibitor (PAI)-2 expression and invasive potential in hepatocellular carcinoma (HCC) cells.
Methods The HCC cell lines with high, low, and non-metastatic potentials, namely MHCC97-H, MHCC97-L, and SMMC-7721 respectively, were cultured in vitro. Matrigel invasion assay and Western blot of PAI-2 protein expression were conducted.
Results The number of invaded cells in MHCC97-L was significantly higher than that in SMMC-7721 (P=0.005), whereas that in MHCC97-H was higher than in MHCC97-L (P=0.017) and SMMC-7721 (P=0.001). Contrarily, PAI-2 protein expression was gradually reducing from SMMC-7721, MHCC97-L, to MHCC97-H (MHCC97-H vs. MHCC97-L, P<0.001; MHCC97-H vs. SMMC-7721, P=0.001; MHCC97-L vs. SMMC-7721, P=0.001). The Pearson’s correlation analysis revealed a significant negative association between invaded cell number and PAI-2 expression (r=-0.892, P=0.001).
Conclusion PAI-2 expression may be negatively associated with the invasive potential of HCC.

Key words: hepatocellular carcinoma, plasminogen activator inhibitor-2, invasive potential

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