Chinese Medical Sciences Journal ›› 2017, Vol. 32 ›› Issue (1): 44-47.doi: 10.24920/J1001-9242.2007.006

• Orginal Article • Previous Articles     Next Articles

Up-regulation of Let-7a Expression Induces Gastric Carcinoma Cell Apoptosis In Vitro

Zhu Yimin, Xu Fuying*()   

  1. Department of Surgery, College of Medicine and Health, Lishui University/Lishui People’s Hospital, Lishui, Zhejiang 323000, China
  • Online:2017-04-11 Published:2017-04-10
  • Contact: Xu Fuying E-mail:zym330422@163.com

Abstract:

Objective To investigate effect of let-7a gene over-expression on apoptosis of gastric cancer cell lines SGC-7901.Methods The stable let-7a gene over-expressing SGC-7901 cells, SGC-7901/let-7a cells, were established using shRNA lentiviral vector methods. Real-time RT-PCR analysis was used to evaluate the expression level of let-7a mRNA. Cells apoptosis was assessed by flow cytometry.Results RT-PCR analysis revealed let-7a expression in SGC-7901/let-7a cells was significantly increased. Cellular apoptosis assay showed that over-expression of let-7a could increase apoptosis of SGC-7901 cells (P=0.002).Conclusion Up-regulating let-7a expression promoted apoptosis in SGC-7901 cells.

Key words: gastric carcinoma, microRNA, apoptosis

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