Chinese Medical Sciences Journal ›› 2017, Vol. 32 ›› Issue (1): 34-43.doi: 10.24920/J1001-9242.2007.005

• Orginal Article • Previous Articles     Next Articles

microRNA-18a Promotes Cell Migration and Invasion Through Inhibiting Dicer l Expression in Hepatocellular Carcinoma In Vitro

Zhang Xiufen1#, Yu Bo2#, Zhang Fuzheng3, Guo Zijian4, *(), Li Lihua1, *()   

  1. 1Institute of Oncology, The Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu 214062, China
    2Department of Oncology, People’s Hospital of Taixing, Taixing, Jiangsu 225400, China
    3Department of Oncology, The Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu 214062, China
    4Department of Surgerical Oncology, The Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu 214062, China
  • Online:2017-04-11 Published:2017-04-10
  • Contact: Guo Zijian,Li Lihua E-mail:gzjwxsy@sina.com;LLHWXSY@aliyun.com
  • Supported by:
    △Supported by the National Natural Science Foundation of China (81472485), the Project of Six Talent Peaks of Jiangsu Province (WSW-014), and Project of Hospital Management Center of Wuxi (YGZXQ1311,YGZ1106, and YGZ1007).

Abstract:

Objective To investigate the effects of microRNA-18a (miR-18a) on migration and invasion of hepatocellular carcinoma (HCC) cells, and its possible mechanism associated with Dicer l.Methods HepG2 and HepG2.2.15 cells were transfected with miR-18a inhibitor using Lipofectamine. Cell invasion was evaluated by transwell invasion assay, and cell migration was detected by transwell migration and wound-healing assays. Moreover, luciferase reporter assay was used to identify whether Dicer expression was regulated by miR-18a. Real-time RT-PCR and western blot were performed to analyze Dicer 1 expression. In addition, a functional restoration assay was performed to investigate whether miR-18a promotes HCC cell migration and invasion by directly targeting Dicer 1.Results miR-18a inhibitor can suppress the migration and invasion of HCC cells. Furthermore, suppression of Dicer l expression by small interfering RNA essentially abolished the inhibition of cell migration and invasion induced by miR-18a inhibitor, restorating these activities to levels similar to the parental HCC cells. Interestingly, suppression of miR-18a in HCC cells resulted in enhanced expression of Dicer l. In addition, the results of a luciferase assay demonstrated targeted regulation of Dicer l by miR-18a.Conclusion Our findings suggest that miR-18a promotes migration and invasion of HCC cells by inhibiting Dicer l expression.

Key words: hepatocellular carcinoma, microRNA-18a, Dicer l, migration, invasion

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