Chinese Medical Sciences Journal ›› 2020, Vol. 35 ›› Issue (1): 85-91.doi: 10.24920/003532

• Original Article • Previous Articles     Next Articles

Association Between Homocysteine Level and Methylenetetrahydrofolate Reductase Gene Polymorphisms in Type 2 Diabetes Accompanied by Dyslipidemia

Yin Ying1, Li Rui2, Li Xiaoli2, Wu Kunrong2, Li Ling2, Xu Yuedong4, Liao Lin4, Yang Rui3, Li Yan3, *()   

  1. 1School of Pharmaceutical Sciences, Shandong First Medical University & Shandong Academy of Medical Sciences, Tai’an, Shandong 271000, China
    2School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China
    3Department of Pharmacy, Shandong Provincial Qianfoshan Hospital, Jinan 250014, China
    4Department of Endocrinology, Shandong Provincial Qianfoshan Hospital, Jinan 250014, China
  • Received:2019-05-12 Online:2020-03-31 Published:2020-04-16
  • Contact: Li Yan E-mail:li_xyan@126.com
  • Supported by:
    This study was supported by the National Key Development Plan for Precision Medicine Research(2017YFC0910004);Jinan Science Project(201602171);Jinan Science and Technology Plan Project(201503009)

Abstract:

Objective To investigate the association between total homocysteine (tHcy) level in plasma and methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C genetic polymorphisms in a Chinese Han nationality population with type 2 diabetes mellitus (T2DM) accompanied by dyslipidemia.
Methods This case-control study enrolled T2DM patients with dyslipidemia and without dyslipidemia respectively. Sanger dideoxy-mediated chain-termination method was used to detect the gene polymorphisms of MTHFR C677T and A1298C. Plasma tHcy and lipid levels were measured as well. The genotype frequency and allele frequency between the dyslipidemia and non-dyslipidemia groups were compared by using Chi-square test. Plasma tHcy level of T2DM patients who carried the different genotypes was compared by Student’s t test.
Results Finally, 82 T2DM patients with dyslipidemia and 94 ones without dyslipidemia were included in this study. There was a significant correlation between tHcy level and MTHFR C677T gene polymorphism in T2DM patients (t=2.27, P=0.02). Moreover, the plasma tHcy level in the dyslipidemia patients who carried MTHFR 677 TT genotype was significantly higher than that in those with CT+CC genotype (13.62±6.97 vs. 10.95±3.62 μmol/L, t=2.20, P=0.03); while for patients without dyslipidemia, comparison of the tHcy level between those who carried the above two alleles showed no significantly difference (13.34±6.03 vs. 12.04±5.09 μmol/L, t=1.08, P=0.29).
Conclusion MTHFR 677TT genotype might associate with higher tHcy level in T2DM patients with dyslipidemia.

Key words: type 2 diabetes mellitus, methylenetetrahydrofolate reductase, polymorphism, homocysteine, hyperlipidemia

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