Chinese Medical Sciences Journal ›› 2023, Vol. 38 ›› Issue (3): 206-217.doi: 10.24920/004198
• Original Article • Previous Articles Next Articles
Chao Huang, Hao-Sheng Liu, Bing-Jun Liang, Sheng-Rong Liao, Wei-Zeng Shen*()
Received:
2022-12-06
Accepted:
2023-06-05
Published:
2023-09-30
Online:
2023-07-04
Contact:
* E-mail:Chao Huang, Hao-Sheng Liu, Bing-Jun Liang, Sheng-Rong Liao, Wei-Zeng Shen. A Chinese Herb Prescription “Fang-gan Decoction” Protects Against Damage to Lung and Colon Epithelial Cells Caused by the SARS-CoV-2 Spike Protein by Regulating the TGF-β/Smad2/3 and NF-κB Pathways[J].Chinese Medical Sciences Journal, 2023, 38(3): 206-217.
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Figure 1.
Pathological observation of lung and colon tissues using HE staining. (A) In the lung tissue, there was thickening and narrowing of the alveolar wall, infiltration of inflammatory cells on the alveolar wall, expansion of the alveolar wall, breakage of the alveolar wall, fusion with each other, and haemorrhage or stasis in the blood vessels (black arrows). In the colon tissue, necrosis and detachment of mucosal epithelial cells and pyknosis of necrotic cells were observed in the mucosal layer. Inflammatory cell infiltration in the lamina propria, loose arrangement of muscle fibres in the muscularis, erosion of mucosal tissue, disappearance of necrotic intestinal glands, and replacement by proliferative connective tissue were observed (yellow arrows). (B) Pathological injury scores of lungs. (C) Pathological injury scores of colon tissues."
Table 1.
Inflammatory cytokine expressions in the serum of mice (by ELISA)"
Markers | Time | Normal group | Model Gorup | NS group | TCM group | P value |
---|---|---|---|---|---|---|
IL-6 | 3d | 644.61 | 717.36* | 696.89* | 702.85* | 0.009 |
5d | 268.02 | 342.09* | 344.88* | 298.89#? | <0.001 | |
IL-10 | 3d | 25.14 | 82.12* | 74.86* | 44.7# | 0.009 |
5d | 87.61 | 145.79* | 123.84* | 121.52 | 0.019 | |
IL-13 | 3d | 352.69 | 344.69 | 362.46 | 351.09 | 0.998 |
5d | 91.9 | 102.87 | 92.42 | 100.23 | 0.931 | |
TNF-α | 3d | 566.19 | 853.87 | 840.04 | 633.38 | 0.141 |
5d | 575.77 | 1042.36* | 1236.73*# | 864.43*#? | <0.001 | |
CRP | 3d | 18175.8 | 21129.27* | 21550.72* | 19786.15 | 0.042 |
5d | 5933.09 | 7413.08* | 6567.13# | 6093.33# | 0.001 |
Figure 4.
Detection of relative proteins, cell viability and permeability. (A) The influence of serum on the viability (Left) and cell permeability (Right) of spike protein-treated hTIIAEC cells. (B) The influence of serum on the S protein-induced expression of caspase 3 and Bcl-2 (protein bands). (C) Comparison of the grey value of caspase 3/GAPDH and Bcl-2/GAPDH. (D) The influence of the serum at different concentrations on the viability of HsIMECs and HCoEpiCs. (E) The effect of serum on the cell permeability of HsIMECs and HCoEpiCs. (F) The influence of serum on the expression of caspase 3 and Bcl-2 in HsIMECs and HCoEpiCs. * P<0.05 vs. the normal control. # P<0.05 vs. the model group."
Table 2.
The effect of serum on spike protein-induced inflammatory cytokines in hTⅡAECs"
Markers | Control group | S model group | S model+10% TCM group | S model+25% TCM group | S model+50% TCM group | P value |
---|---|---|---|---|---|---|
IL-6 (ng/L) | 20.24 | 33.01* | 29.81*# | 27.95*# | 20.01# | <0.001 |
IL-10 (ng/L) | 428.32 | 435.40 | 435.54 | 435.38 | 418.36 | 0.762 |
IL-13 (ng/L) | 41.33 | 27.17* | 30.75*# | 33.67*# | 35.04*# | <0.001 |
TNF-α (ng/L) | 212.14 | 309.29* | 277.51* | 263.84 | 222.83 | <0.001 |
NF-κB (ng/L) | 215.69 | 187.85 | 183.57 | 206.98 | 213.29 | 0.154 |
TGF-β1 (ng/L) | 285.49 | 156.97* | 185.03*# | 196.94*# | 272.26# | <0.001 |
Table 3.
The relative levels of inflammatory cytokines in the supernatants of HsIMECs and HCoEpiCs by ELISA"
Cells | Markers | Control group | S model group | S model+10% TCM group | S model+25% TCM group | S model+50% TCM group | P value |
---|---|---|---|---|---|---|---|
HsIMECs | IL-6 (ng/L) | 22.40 | 30.49* | 27.62* | 25.39# | 22.11# | <0.001 |
IL-10 (ng/L) | 348.14 | 307.22* | 318.12* | 327.84*# | 339.72# | 0.001 | |
IL-13 (ng/L) | 30.47 | 30.59 | 28.49 | 31.09 | 29.16 | 0.757 | |
TNF-α (ng/L) | 121.90 | 273.29* | 271.30* | 234.02* | 202.70*# | <0.001 | |
NF-κB (ng/L) | 178.52 | 177.08 | 175.88 | 178.74 | 180.86 | 0.859 | |
TGF-β1 (ng/L) | 149.71 | 146.78 | 143.00 | 141.65 | 145.37 | 0.986 | |
HCoEpiCs | IL-6 (ng/L) | 24.13 | 28.28* | 25.26# | 23.78# | 21.95# | 0.001 |
IL-10 (ng/L) | 372.10 | 384.02 | 378.86 | 388.56 | 388.92 | 0.910 | |
IL-13 (ng/L) | 23.69 | 15.99* | 20.01 | 22.77# | 21.53# | 0.021 | |
TNF-α (ng/L) | 168.63 | 286.16* | 281.62* | 230.50*# | 221.83*# | <0.001 | |
NF-κB (ng/L) | 133.02 | 131.88 | 134.09 | 126.03 | 134.28 | 0.201 | |
TGF-β1 (ng/L) | 177.31 | 173.80 | 176.35 | 177.77 | 171.37 | 0.452 |
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