Chinese Medical Sciences Journaldoi: 10.24920/004340
崔悦1#, 王宏志1#, 宋烨1, 杨爽1, 赛凤英2, 于德军2,*
收稿日期:
2024-01-16
接受日期:
2024-04-18
发布日期:
2024-05-20
通讯作者:
*于德军,yudejun100@126.com.
作者简介:
#共同第一作者
Yue Cui1#, Hong-Zhi Wang1#, Ye Song1, Shuang Yang1, Feng-Ying Sai2, De-Jun Yu2,*
Received:
2024-01-16
Accepted:
2024-04-18
Online:
2024-05-20
Contact:
*De-Jun Yu, E-mail: About author:
#Co-first authors.
摘要: 目的 利用基因表达综合数据库(Gene Expression Omnibus,GEO)筛选靶基因UBE2C,并基于癌症基因组图谱(Cancer Genome Atlas, TCGA)的多个公共生物信息学研究平台探讨UBE2C在乳腺癌中的预后价值及免疫相关性。
方法 从GEO下载乳腺癌基因芯片表达数据集,分析获得差异表达基因(differentially expressed genes,DEGs)。通过构建和可视化DEG-蛋白相互作用网络获得枢纽基因。然后利用R语言、STRING和Cytoscape工具确定关键基因UBE2C,并利用外部数据集、TCGA和定量实时聚合酶链式反应(quantitative real-time polymerase chain reaction,qRT-PCR)验证UBE2C差异表达。使用R语言、TIMER和GSEA工具研究UBE2C在乳腺癌中的预后价值和免疫学相关性。
结果 GEO独立数据集、TCGA和qRT-PCR均证实UBE2C在乳腺癌组织中差异上调。预后分析显示UBE2C是一个独立的预后因素。UBE2C高表达降低乳腺癌组织中B细胞、CD4+T细胞、CD8+T细胞、巨噬细胞和髓系树突状细胞免疫浸润水平。UBE2C在乳腺癌中的表达与PDCD1、CD274和CTLA4的表达显著相关。同时,UBE2C的表达与肿瘤突变负荷水平和微卫星不稳定性水平呈显著正相关。基因集富集分析显示,在786个免疫相关基因集中UBE2C表达显著富集。
结论 UBE2C在乳腺癌组织中的表达可预测乳腺癌患者的生存和预后。此外,UBE2C与乳腺癌免疫微环境密切相关,其在乳腺癌中的表达可用于预测乳腺癌患者免疫治疗的效果。因此,UBE2C在乳腺癌的发生和进展中起着至关重要的作用,是一种潜在的免疫相关的预后生物标志物。
Yue Cui, Hong-Zhi Wang, Ye Song, Shuang Yang, Feng-Ying Sai, De-Jun Yu. UBE2C as an Immune-Related Biomarker for Breast Cancer: A Study Based on Multiple Databases[J/OL].Chinese Medical Sciences Journal, .DOI:10.24920/004340.[Epub ahead of print].
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