Chinese Medical Sciences Journal ›› 2018, Vol. 33 ›› Issue (4): 252-259.doi: 10.24920/003477

• Original Article • Previous Articles     Next Articles

Longitudinal Measurement of Hemodynamic Changes within the Posterior Optic Nerve Head in Rodent Nonarteritic Anterior Ischemic Optic Neuropathy

Ma Jin1, Chen Ting1, 2, Wang Yiwei1, Zhao Chan1, Li Donghui1, Wang Meng1, Gan Linyang1, Zhong Yong1, *   

  1. 1 Department of Ophthalmology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China;
    2 Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan, 430060 China
  • Received:2018-05-07 Accepted:2018-09-03 Published:2018-12-30 Online:2018-12-10
  • Contact: Zhong Yong

Objective To assess the in vivo dynamic blood flow features of posterior optic nerve head (ONH) in rat model of nonarteritic anterior ischemic optic neuropathy (rNAION). Methods rNAION was established with Rose Bengal and argon green laser in Sprague-Dawley rats. Fundus photography and fundus fluorescein angiography (FFA) were performed to assess the dynamic changes of optic disc in morphology in 90 days and in blood perfusion in 3 hours after the induction of disease. Histological examinations were performed to evaluate the success of modeling. The dynamic blood flow kinetics of posterior ONH in rNAION were measured by Laser Doppler Flowmetry (LDF) on the day 3, 7, 14, 21, and 40 after the disease induction. One-way ANOVA, Student’s t-test and Bonferroni adjustment were used for multiple comparisons of kinetic measurements of blood flow. Results Optic disc edema and subsequent resolution associated with the development of optic disc pallor were observed in rNAION. FFA showed that the optic disc was hypofluorescence in the early phase and hyperfluorescence in the late phase. Histological studies suggested edema and loosened tissues of ONH, loss of retinal ganglion cells (RGCs), optic nerve substance and gliosis. Compared to the naive rats, the blood flow kinetics of posterior ONH in rNAION significant reduced at each time point after modeling (F=175.06, P<0.0001). The reductions were specifically remarkable in 14 days after the disease induction (All P<0.01). Conclusions Continuous blood perfusion reduction was found in rNAION, with significant alteration in 14 days after disease induction. Our results provided important information for understanding the hemodynamic changes in rNAION.

Key words: nonarteritic anterior ischemic optic neuropathy, rat model, posterior optic nerve head, blood flow, in vivo

Funding: Supported by the National Natural Science Foundation(81670854)

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