Chinese Medical Sciences Journal ›› 2020, Vol. 35 ›› Issue (2): 142-150.doi: 10.24920/003643
• Original Article • Previous Articles Next Articles
Targeted MR Imaging Adopting T1-Weighted Ultra-Small Iron Oxide Nanoparticles for Early Hepatocellular Carcinoma: An in vitro and in vivo Study
Xu Yanhong, Yang Jia, Meng Jie, Wang Han(
)
- Department of Radiology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200080, China
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Received:2019-07-26Accepted:2019-12-10Published:2020-06-30Online:2020-06-09 -
Contact:Wang Han E-mail:13564112852@163.com
Cite this article
Xu Yanhong,Yang Jia,Meng Jie,Wang Han. Targeted MR Imaging Adopting T1-Weighted Ultra-Small Iron Oxide Nanoparticles for Early Hepatocellular Carcinoma: An in vitro and in vivo Study[J].Chinese Medical Sciences Journal, 2020, 35(2): 142-150.
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Figure 1.
Schematic illustration of synthesis of Fe3O4-PEG-RGD as a platform for targeted magnetic resonance imaging of HepG2 cells."
Figure 1.
Figure 2.
The in vitro T1-weighted MR images of the Fe3O4-mPEG (non-targeted) and Fe3O4-PEG-RGD (targeted) NPs at Fe concentration of 0.05, 0.1, 0.2, 0.4, 0.8 mM, respectively."
Figure 2.
Figure 3.
MTT assay of cell viability 24 hours after the treatment of Fe3O4-PEG-RGD NPs. PBS were used as control (n=3). A. HepG2 cells; B. 293T cells."
Figure 3.
Figure 4.
In vivo T1-weighted MR image of human hepatoma cell HepG2 acquired 2 hours after treated with the Fe3O4-mPEG (non-targeted) and Fe3O4-PEG-RGD (targeted) NPs at Fe concentrations of 10, 25, 50, 75, and 100 μg/mL, respectively."
Figure 4.
Figure 5.
In vivo T1-weighted MR imaging of HepG2 tumor model mice before and after injection of the Fe3O4-mPEG (non-targeted) and Fe3O4-PEG-RGD (targeted) NPs solution through tail vein. The arrows indicate the tumorous area."
Figure 5.
Figure 6.
The in vivo biodistribution of Fe in tumor and different organs of nude mice received injection of Fe3O4-mPEG (non-targeted) or Fe3O4-PEG-RGD (targeted) NPs (450 μg Fe, in 0.3 mL PBS)."
Figure 6.
Figure 7.
Transmission electron microscopy images of hepatocellular carcinoma in Fe3O4-PEG-RGD (targeted) NPs treated mice. A. Image of a hepatocellular carcinoma cell (10000×); B. a higher-magnified image of the highlight area on image (A) showing the distribution of Fe3O4-PEG-RGD NPs in the lysosomes (arrow) (30000×)."
Figure 7.
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